PMID- 24743310
OWN - NLM
STAT- MEDLINE
DCOM- 20150605
LR  - 20220408
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 4
DP  - 2014
TI  - Expression of stanniocalcin-1 and stanniocalcin-2 in laryngeal squamous cell 
      carcinoma and correlations with clinical and pathological parameters.
PG  - e95466
LID - 10.1371/journal.pone.0095466 [doi]
LID - e95466
AB  - BACKGROUND: Stanniocalcin-1 (STC1) and stanniocalcin-2 (STC2) are secreted 
      glycoprotein hormones involved in various types of human malignancies. The roles 
      of STC1 and STC2 in laryngeal squamous cell carcinoma (LSCC) remain unknown. We 
      investigated correlations between STC1 and STC2 expression and 
      clinicopathological or prognostic factors in LSCC. METHODS: Pre-surgical 
      peripheral blood samples were collected between 2012 and 2013 from 62 patients 
      with LSCC. Quantitative RT-PCR analysis was performed to examine mRNA levels of 
      STC1 and STC2. Immunohistochemistry was performed to retrospectively analyze 90 
      paraffin-embedded LSCC tissue samples, which were obtained from patients who 
      received surgery between 2006 and 2009. These patients did not have histories of 
      treatment or malignancies. Univariate analysis of patient survival was performed 
      by the Kaplan-Meier method. Multivariate analyses were performed with the Cox 
      proportional hazards model. RESULTS: The relative mRNA levels of STC1 and STC2 in 
      peripheral blood were significantly greater in LSCC patients than those of 
      healthy volunteers (both P<0.05). STC2 protein expression in tumor tissues was 
      associated with invasion into the thyroid cartilage, T-Stage, lymphatic 
      metastasis, clinical stage, and pathological differentiation (all P<0.05). In 
      addition, STC2 protein expression was an independent prognostic factor for 
      overall survival in patients with LSCC (P = 0.025). In contrast, STC1 expression 
      only correlated with clinical stage (P = 0.026) and was not an independent or 
      significant prognostic factor. CONCLUSIONS: Circulating STC1 and STC2 mRNA are 
      potentially useful blood markers for LSCC. Our results strongly suggest that the 
      STC2 protein, but not STC1, may be a valuable biomarker for LSCC malignancies and 
      a prognostic marker for poor outcome following surgery. Future studies should 
      examine STC2 as a novel molecular target for the treatment of LSCC.
FAU - Zhou, Han
AU  - Zhou H
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital, Nanjing Medical 
      University, Nanjing, China.
FAU - Li, Ying-Ying
AU  - Li YY
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital, Nanjing Medical 
      University, Nanjing, China.
FAU - Zhang, Wei-Qiang
AU  - Zhang WQ
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital, Nanjing Medical 
      University, Nanjing, China.
FAU - Lin, Dan
AU  - Lin D
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital, Nanjing Medical 
      University, Nanjing, China.
FAU - Zhang, Wei-Ming
AU  - Zhang WM
AD  - Department of Pathology, The First Affiliated Hospital, Nanjing Medical 
      University, Nanjing, China.
FAU - Dong, Wei-Da
AU  - Dong WD
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital, Nanjing Medical 
      University, Nanjing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140417
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Glycoproteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (STC2 protein, human)
RN  - 76687-96-2 (teleocalcin)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell/genetics/*metabolism/pathology
MH  - Female
MH  - Glycoproteins/genetics/*metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Intercellular Signaling Peptides and Proteins/genetics/*metabolism
MH  - Laryngeal Neoplasms/genetics/*metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Reverse Transcriptase Polymerase Chain Reaction
PMC - PMC3990672
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/04/20 06:00
MHDA- 2015/06/06 06:00
PMCR- 2014/04/17
CRDT- 2014/04/19 06:00
PHST- 2013/12/23 00:00 [received]
PHST- 2014/03/27 00:00 [accepted]
PHST- 2014/04/19 06:00 [entrez]
PHST- 2014/04/20 06:00 [pubmed]
PHST- 2015/06/06 06:00 [medline]
PHST- 2014/04/17 00:00 [pmc-release]
AID - PONE-D-13-54314 [pii]
AID - 10.1371/journal.pone.0095466 [doi]
PST - epublish
SO  - PLoS One. 2014 Apr 17;9(4):e95466. doi: 10.1371/journal.pone.0095466. eCollection 
      2014.