PMID- 2474437
OWN - NLM
STAT- MEDLINE
DCOM- 19890920
LR  - 20131121
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 125
IP  - 3
DP  - 1989 Sep
TI  - Estradiol regulation of sterol carrier protein-2 independent of cytochrome P450 
      side-chain cleavage expression in the rat corpus luteum.
PG  - 1337-44
AB  - A major action of estradiol in the corpus luteum of the pregnant rat is to 
      increase the supply of cholesterol substrate for progesterone production by 
      stimulating both cholesterol synthesis and uptake. To determine whether this 
      steroid also affects cholesterol metabolism and transport, estradiol's action on 
      the expression of cytochrome P450 side-chain cleavage enzyme (P450scc) and the 
      cholesterol transport protein, sterol carrier protein-2 (SCP2), was examined. 
      Mitochondria isolated from corpora lutea of estradiol-treated rats secreted 
      significantly more progestagen than mitochondria of control corpora lutea. 
      Several findings indicate that estradiol enhances cholesterol transport and 
      availability to the P450scc rather than affects the expression of this enzyme: 1) 
      the difference in mitochondrial progestagen synthesis induced by estradiol was 
      obliterated by the presence of 25-hydroxycholesterol; 2) immunoblotting of 
      P450scc indicated no stimulatory effect of estradiol on the amount of enzyme; and 
      3) levels of P450scc mRNA were not increased by estradiol. Whereas estradiol had 
      no stimulatory effect on P450scc it caused a mark (3-fold) increase in the 
      mitochondrial content of SCP2. Thus, the increase in luteal progestagen synthesis 
      stimulated by estradiol appears to be associated with an increase in 
      mitochondrial SCP2 and is independent of luteal P450 content or message.
FAU - McLean, M P
AU  - McLean MP
AD  - Department of Physiology and Biophysics, University of Illinois College of 
      Medicine, Chicago 60612.
FAU - Puryear, T K
AU  - Puryear TK
FAU - Khan, I
AU  - Khan I
FAU - Azhar, S
AU  - Azhar S
FAU - Billheimer, J T
AU  - Billheimer JT
FAU - Orly, J
AU  - Orly J
FAU - Gibori, G
AU  - Gibori G
LA  - eng
GR  - HD-11119/HD/NICHD NIH HHS/United States
GR  - HD-6982/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Carrier Proteins)
RN  - 0 (Plant Proteins)
RN  - 0 (Sterols)
RN  - 0 (sterol carrier proteins)
RN  - 3XMK78S47O (Testosterone)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 63231-63-0 (RNA)
RN  - EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Carrier Proteins/*metabolism
MH  - Cholesterol Side-Chain Cleavage Enzyme/biosynthesis/*genetics
MH  - Corpus Luteum/drug effects/*enzymology/physiology
MH  - Estradiol/*pharmacology
MH  - Female
MH  - Immunoblotting
MH  - Mitochondria/enzymology
MH  - Nucleic Acid Hybridization
MH  - Organ Size/drug effects
MH  - *Plant Proteins
MH  - Progesterone/biosynthesis
MH  - RNA/genetics/isolation & purification
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Sterols/metabolism
MH  - Testosterone/pharmacology
EDAT- 1989/09/01 00:00
MHDA- 1989/09/01 00:01
CRDT- 1989/09/01 00:00
PHST- 1989/09/01 00:00 [pubmed]
PHST- 1989/09/01 00:01 [medline]
PHST- 1989/09/01 00:00 [entrez]
AID - 10.1210/endo-125-3-1337 [doi]
PST - ppublish
SO  - Endocrinology. 1989 Sep;125(3):1337-44. doi: 10.1210/endo-125-3-1337.