PMID- 24744643
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140418
LR  - 20211021
IS  - 1300-7777 (Print)
IS  - 1308-5263 (Electronic)
IS  - 1300-7777 (Linking)
VI  - 29
IP  - 2
DP  - 2012 Jun
TI  - Molecular Evaluation of t(14;18)(bcl-2/IgH) Translocation in Follicular Lymphoma 
      at Diagnosis Using Paraffin-Embedded Tissue Sections.
PG  - 126-34
LID - 10.5505/tjh.2012.93898 [doi]
AB  - OBJECTIVE: Follicular lymphoma (FL) is one of the most common lymphomas, and is 
      characterized by t(14;18)(q32;q21) in more than 80% of patients. The aim of this 
      study was to determine the rate of t(14;18) positivity based onthe detection of 
      mbr or mcr in paraffin-embedded tissue samples. MATERIAL AND METHODS: The study 
      included 32 paraffin-embedded tissue samples collected from 32 consecutive FL 
      patients that were diagnosed and followed-up at our hospital between 1999 and 
      2006. The MBR breakpoint wasidentified based on real-time PCR using a LightCycler 
      v.2.0 t(14;18) Quantification Kit (MBR), multiplex PCR, and seminestedPCR. To 
      identify the mcr breakpoint, real-time PCR was performed using specific primers 
      and the FastStart DNAMaster SYBR Green I Kit. To detect t(14;18) via fluorescence 
      in situ hybridization (FISH) nuclei from paraffin-embeddedtissue sections were 
      extracted and used together with LSI IgH (immunoglobulin heavy chain) (spectrum 
      green)/bcl-2(B-cell leukemia-lymphoma 2) (spectrum orange) probes. RESULTS: The 
      DNA and nuclei isolation success rate for B5 formalin-fixed, paraffin-embedded 
      tissue sections (n = 12)was 42% and 33%, respectively, versus 95% and 60%, 
      respectively, for 20 tissue sections fixed in formalin only. In all,24 
      paraffin-embedded tissue sections were analyzed and mbr positivity was observed 
      in the DNA of 82.14% via seminested PCR, in 53.57% via multiplex PCR, and in 
      28.57% via real-time PCR. We did not detect mcr rearrangementin any of the 
      samples. In all, 15 of 16 patients (93.75%) whose nuclei were successfully 
      isolated were observed to bet(14;18) positive via the FISH method. CONCLUSION: 
      Semi-nested PCR and FISH facilitated the genetic characterization of FL tumors. 
      As such, FISH and PCR complement each other and are both essential for detecting 
      t(14;18) translocation.
FAU - Selvi, Nur
AU  - Selvi N
AD  - Ege University, School of Medicine, Medical Biology Department, Izmir, Turkey.
FAU - Kosova, Buket
AU  - Kosova B
AD  - Ege University, School of Medicine, Medical Biology Department, Izmir, Turkey.
FAU - Hekimgil, Mine
AU  - Hekimgil M
AD  - Ege University, School of Medicine, Pathology Department, Izmir, Turkey.
FAU - Gunduz, Cumhur
AU  - Gunduz C
AD  - Ege University, School of Medicine, Medical Biology Department, Izmir, Turkey.
FAU - Kaymaz, Burcin Tezcanli
AU  - Kaymaz BT
AD  - Ege University, School of Medicine, Medical Biology Department, Izmir, Turkey.
FAU - Karaca, Emin
AU  - Karaca E
AD  - Ege University, School of Medicine, Medical Genetics Department, Izmir, Turkey.
FAU - Saydam, Guray
AU  - Saydam G
AD  - Ege University, School of Medicine, Hematology Department, Izmir, Turkey.
FAU - Tombuloglu, Murat
AU  - Tombuloglu M
AD  - Ege University, School of Medicine, Hematology Department, Izmir, Turkey.
FAU - Buyukkececi, Filiz
AU  - Buyukkececi F
AD  - Ege University, School of Medicine, Hematology Department, Izmir, Turkey.
FAU - Cagirgan, Seckin
AU  - Cagirgan S
AD  - Ege University, School of Medicine, Hematology Department, Izmir, Turkey.
FAU - Ertan, Yesim
AU  - Ertan Y
AD  - Ege University, School of Medicine, Pathology Department, Izmir, Turkey.
FAU - Topcuoglu, Nejat
AU  - Topcuoglu N
AD  - Ege University, School of Medicine, Medical Biology Department, Izmir, Turkey.
LA  - eng
PT  - Journal Article
DEP - 20120615
PL  - Turkey
TA  - Turk J Haematol
JT  - Turkish journal of haematology : official journal of Turkish Society of 
      Haematology
JID - 9606065
PMC - PMC3986950
OTO - NOTNLM
OT  - FISH
OT  - Follicular lymphoma
OT  - Multiplex PCR
OT  - Semi-nested PCR
EDAT- 2012/06/01 00:00
MHDA- 2012/06/01 00:01
PMCR- 2012/06/01
CRDT- 2014/04/19 06:00
PHST- 2010/06/22 00:00 [received]
PHST- 2011/03/05 00:00 [accepted]
PHST- 2014/04/19 06:00 [entrez]
PHST- 2012/06/01 00:00 [pubmed]
PHST- 2012/06/01 00:01 [medline]
PHST- 2012/06/01 00:00 [pmc-release]
AID - 380 [pii]
AID - 10.5505/tjh.2012.93898 [doi]
PST - ppublish
SO  - Turk J Haematol. 2012 Jun;29(2):126-34. doi: 10.5505/tjh.2012.93898. Epub 2012 
      Jun 15.