PMID- 24744892
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140418
LR  - 20211021
IS  - 2051-817X (Print)
IS  - 2051-817X (Electronic)
IS  - 2051-817X (Linking)
VI  - 2
IP  - 2
DP  - 2014 Feb 1
TI  - Catalyzing role of erythropoietin on the nitric oxide central pathway during the 
      ventilatory responses to hypoxia.
PG  - e00223
LID - 10.1002/phy2.223 [doi]
LID - e00223
AB  - The N-Methyl-d-Aspartate (NMDA) receptors - neuronal nitric oxide synthase (nNOS) 
      pathway is involved in the ventilatory response to hypoxia. The objective was to 
      assess the possible effect of erythropoietin deficiency and chronic exposure to 
      hypoxia on this pathway during ventilatory response to acute hypoxia. Wild-type 
      (WT) and erythropoietin-deficient (Epo-TAg(h)) male mice were exposed (14 days) 
      either to hypobaric hypoxia (Pb = 435 mmHg) or to normoxia. The ventilation was 
      measured at 21% or 8% O2 after injection of vehicle (NaCl), nNOS inhibitor (SMTC) 
      or NMDA receptor antagonist (MK-801). Nitric oxide production and the expression 
      of NMDA receptor and nNOS were assessed by real-time RT-PCR and Western blot 
      analyses in the medulla. At rest, Epo-TAg(h) mice displayed normal ventilatory 
      parameters at 21% O2 but did not respond to acute hypoxia despite a larger 
      expression of NMDA receptors and nNOS in the medulla. Ventilatory acclimatization 
      to hypoxia was observed in WT but was absent in Epo-TAg(h) mice. nNOS inhibition 
      blunted the hypoxic ventilatory acclimatization of WT mice without any effect in 
      Epo-TAg(h) mice. Acute hypoxic ventilatory response (HVR) was increased after 
      chronic hypoxia in WT but remained unchanged in Epo-TAg(h) mice. Ventilatory 
      response to acute hypoxia was modified by MK-801 injection in WT and Epo-TAg(h) 
      mice. The results confirm that adequate erythropoietin level is necessary to 
      obtain an appropriate HVR and a significant ventilatory acclimatization to 
      hypoxia. Furthermore, erythropoietin plays a potential catalyzing role in the 
      NMDA-NO central pathway during the ventilatory response and acclimatization to 
      hypoxia.
FAU - Voituron, Nicolas
AU  - Voituron N
AD  - Laboratoire "Reponses cellulaires et fonctionnelles a l'hypoxie", Universite 
      Paris 13, Sorbonne Paris Cite, UFR SMBH, EA 2363, Bobigny, 93017, France ; 
      Laboratory of Excellence (Labex) GR-Ex, PRES Sorbonne Paris Cite.
FAU - Jeton, Florine
AU  - Jeton F
AD  - Laboratoire "Reponses cellulaires et fonctionnelles a l'hypoxie", Universite 
      Paris 13, Sorbonne Paris Cite, UFR SMBH, EA 2363, Bobigny, 93017, France ; 
      Laboratory of Excellence (Labex) GR-Ex, PRES Sorbonne Paris Cite.
FAU - Cholley, Yannick
AU  - Cholley Y
AD  - Laboratoire "Reponses cellulaires et fonctionnelles a l'hypoxie", Universite 
      Paris 13, Sorbonne Paris Cite, UFR SMBH, EA 2363, Bobigny, 93017, France.
FAU - Hasnaoui-Saadani, Raja El
AU  - Hasnaoui-Saadani RE
AD  - Laboratoire "Reponses cellulaires et fonctionnelles a l'hypoxie", Universite 
      Paris 13, Sorbonne Paris Cite, UFR SMBH, EA 2363, Bobigny, 93017, France.
FAU - Marchant, Dominique
AU  - Marchant D
AD  - Laboratoire "Reponses cellulaires et fonctionnelles a l'hypoxie", Universite 
      Paris 13, Sorbonne Paris Cite, UFR SMBH, EA 2363, Bobigny, 93017, France.
FAU - Quidu, Patricia
AU  - Quidu P
AD  - Laboratoire "Reponses cellulaires et fonctionnelles a l'hypoxie", Universite 
      Paris 13, Sorbonne Paris Cite, UFR SMBH, EA 2363, Bobigny, 93017, France.
FAU - Favret, Fabrice
AU  - Favret F
AD  - Laboratoire "Reponses cellulaires et fonctionnelles a l'hypoxie", Universite 
      Paris 13, Sorbonne Paris Cite, UFR SMBH, EA 2363, Bobigny, 93017, France.
FAU - Richalet, Jean-Paul
AU  - Richalet JP
AD  - Laboratoire "Reponses cellulaires et fonctionnelles a l'hypoxie", Universite 
      Paris 13, Sorbonne Paris Cite, UFR SMBH, EA 2363, Bobigny, 93017, France ; 
      Laboratory of Excellence (Labex) GR-Ex, PRES Sorbonne Paris Cite.
FAU - Pichon, Aurelien
AU  - Pichon A
AD  - Laboratoire "Reponses cellulaires et fonctionnelles a l'hypoxie", Universite 
      Paris 13, Sorbonne Paris Cite, UFR SMBH, EA 2363, Bobigny, 93017, France ; 
      Laboratory of Excellence (Labex) GR-Ex, PRES Sorbonne Paris Cite.
LA  - eng
PT  - Journal Article
DEP - 20140214
PL  - United States
TA  - Physiol Rep
JT  - Physiological reports
JID - 101607800
PMC - PMC3966246
OTO - NOTNLM
OT  - Erythropoietin
OT  - NMDA receptors
OT  - hypoxia
EDAT- 2014/04/20 06:00
MHDA- 2014/04/20 06:01
PMCR- 2014/02/14
CRDT- 2014/04/19 06:00
PHST- 2013/10/30 00:00 [received]
PHST- 2014/01/09 00:00 [revised]
PHST- 2014/01/11 00:00 [accepted]
PHST- 2014/04/19 06:00 [entrez]
PHST- 2014/04/20 06:00 [pubmed]
PHST- 2014/04/20 06:01 [medline]
PHST- 2014/02/14 00:00 [pmc-release]
AID - phy2223 [pii]
AID - 10.1002/phy2.223 [doi]
PST - epublish
SO  - Physiol Rep. 2014 Feb 14;2(2):e00223. doi: 10.1002/phy2.223. eCollection 2014 Feb 
      1.