PMID- 24745647
OWN - NLM
STAT- MEDLINE
DCOM- 20150626
LR  - 20140506
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 86
IP  - 9
DP  - 2014 May 6
TI  - Polypyrrolic bipyridine bis(phenantrolinequinone) Ru(II) complex/carbon nanotube 
      composites for NAD-dependent enzyme immobilization and wiring.
PG  - 4409-15
LID - 10.1021/ac500272v [doi]
AB  - We report the synthesis and electrochemical characterization of a novel 
      electropolymerizable Ru(II) complex containing two phenanthrolinequinone ligands, 
      Ru(II)(PhQ)2(bpy-pyrrole)(PF6)2. This complex was electropolymerized on glassy 
      carbon (GC) and multiwalled carbon nanotube (MWCNT) electrodes. Higher apparent 
      surface concentrations (80 nmol cm(-2)) were obtained on MWCNTs than on GC 
      electrodes and correspond to  approximately 1000 equivalent compact monolayers of Ru complex. 
      Moreover, the nanostructured metallopolymer exhibits efficient electrocatalytic 
      properties toward oxidation of NADH. This metallopolymer can be electrogenerated 
      in water from the adsorbed Ru(II) monomer. This property was applied to the 
      immobilization of enzymes by coadsorption of Ru complex and enzyme and then 
      electropolymerization of coatings. This two-step procedure leads to the 
      entrapment of 70%-90% of the deposited amount of enzyme in 
      poly-Ru(II)(PhQ)2(bpy-pyrrole) films. Glucose dehydrogenase (GDH) was thus 
      efficiently immobilized in the electrogenerated polymer matrix. In presence of 
      NAD(+) (10 mM), the resulting enzyme electrode exhibits high current densities 
      for glucose oxidation of 1.04 mA cm(-2) at low overpotentials (-0.1 V) with a 
      detection limit of 1 muM of glucose.
FAU - Reuillard, Bertrand
AU  - Reuillard B
AD  - Universite Grenoble Alpes , DCM UMR 5250, F-38000 Grenoble, France.
FAU - Le Goff, Alan
AU  - Le Goff A
FAU - Cosnier, Serge
AU  - Cosnier S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140418
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Nanotubes, Carbon)
RN  - 0 (Polymers)
RN  - 0 (Pyrroles)
RN  - 0 (Quinones)
RN  - 0U46U6E8UK (NAD)
RN  - 30604-81-0 (polypyrrole)
RN  - 7UI0TKC3U5 (Ruthenium)
SB  - IM
MH  - NAD/*chemistry
MH  - *Nanotubes, Carbon
MH  - Polymers/*chemistry
MH  - Pyrroles/*chemistry
MH  - Quinones/*chemistry
MH  - Ruthenium/*chemistry
EDAT- 2014/04/22 06:00
MHDA- 2015/06/27 06:00
CRDT- 2014/04/22 06:00
PHST- 2014/04/22 06:00 [entrez]
PHST- 2014/04/22 06:00 [pubmed]
PHST- 2015/06/27 06:00 [medline]
AID - 10.1021/ac500272v [doi]
PST - ppublish
SO  - Anal Chem. 2014 May 6;86(9):4409-15. doi: 10.1021/ac500272v. Epub 2014 Apr 18.