PMID- 24745735
OWN - NLM
STAT- MEDLINE
DCOM- 20150819
LR  - 20140421
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 820
DP  - 2014 Apr 11
TI  - Rapid screening of multiple antibiotic residues in milk using disposable 
      amperometric magnetosensors.
PG  - 32-8
LID - S0003-2670(14)00308-0 [pii]
LID - 10.1016/j.aca.2014.03.005 [doi]
AB  - Disposable amperometric magnetosensors, involving a mixture of modified-magnetic 
      beads (MBs), for the multiplex screening of cephalosporins (CPHs), sulfonamides 
      (SAs) and tetracyclines (TCs) antibiotic residues in milk are reported for the 
      first time in this work. The multiplexed detection relies on the use of a mixture 
      of target specific modified magnetic beads (MBs) and application of direct 
      competitive assays using horseradish peroxidase (HRP)-labeled tracers. The 
      amperometric responses measured at -0.20 V vs. the Ag pseudo-reference electrode 
      of screen-printed carbon electrodes (SPCE) upon the addition of H2O2 in the 
      presence of hydroquinone (HQ) as redox mediator, were used to monitor the extent 
      of the different affinity reactions. The developed methodology, involving a 
      simple and short pretreatment, allowed discrimination between no contaminated UHT 
      and raw milk samples and samples containing antibiotic residues at the maximum 
      residue limits (MRLs). The usefulness of the multiplexed magnetosensor was 
      demonstrated by analyzing spiked milk samples in only 5 min. The results 
      demonstrated that a clear discrimination of milk samples contaminated with 
      antibiotics at their MRL level or their mixtures, allowing the identification of 
      milk not complying with current legislation. These features make the developed 
      methodology a promising alternative in the development of user-friendly devices 
      for on-site analysis to ensure quality control for dairy products.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Conzuelo, F
AU  - Conzuelo F
AD  - Departamento de Quimica Analitica, Facultad de Ciencias Quimicas, Universidad 
      Complutense de Madrid, Avenida Complutense s/n, 28040 Madrid, Spain. Electronic 
      address: felipecf@quim.ucm.es.
FAU - Ruiz-Valdepenas Montiel, V
AU  - Ruiz-Valdepenas Montiel V
AD  - Departamento de Quimica Analitica, Facultad de Ciencias Quimicas, Universidad 
      Complutense de Madrid, Avenida Complutense s/n, 28040 Madrid, Spain. Electronic 
      address: victor_lega90@hotmail.com.
FAU - Campuzano, S
AU  - Campuzano S
AD  - Departamento de Quimica Analitica, Facultad de Ciencias Quimicas, Universidad 
      Complutense de Madrid, Avenida Complutense s/n, 28040 Madrid, Spain. Electronic 
      address: susanacr@quim.ucm.es.
FAU - Gamella, M
AU  - Gamella M
AD  - Departamento de Quimica Analitica, Facultad de Ciencias Quimicas, Universidad 
      Complutense de Madrid, Avenida Complutense s/n, 28040 Madrid, Spain. Electronic 
      address: mariagam@quim.ucm.es.
FAU - Torrente-Rodriguez, R M
AU  - Torrente-Rodriguez RM
AD  - Departamento de Quimica Analitica, Facultad de Ciencias Quimicas, Universidad 
      Complutense de Madrid, Avenida Complutense s/n, 28040 Madrid, Spain. Electronic 
      address: rebeca.magnolia@gmail.com.
FAU - Reviejo, A J
AU  - Reviejo AJ
AD  - Departamento de Quimica Analitica, Facultad de Ciencias Quimicas, Universidad 
      Complutense de Madrid, Avenida Complutense s/n, 28040 Madrid, Spain. Electronic 
      address: reviejo@quim.ucm.es.
FAU - Pingarron, J M
AU  - Pingarron JM
AD  - Departamento de Quimica Analitica, Facultad de Ciencias Quimicas, Universidad 
      Complutense de Madrid, Avenida Complutense s/n, 28040 Madrid, Spain. Electronic 
      address: pingarro@quim.ucm.es.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140312
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Anti-Bacterial Agents)
RN  - EC 1.11.1.- (Horseradish Peroxidase)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis/chemistry
MH  - Biosensing Techniques/*instrumentation
MH  - *Disposable Equipment
MH  - Drug Residues/*analysis/chemistry
MH  - Electrochemistry
MH  - Electrodes
MH  - Horseradish Peroxidase/metabolism
MH  - Magnets/*chemistry
MH  - Microspheres
MH  - Milk/*chemistry
MH  - Time Factors
OTO - NOTNLM
OT  - Cephalosporins
OT  - Magnetic beads
OT  - Milk
OT  - Screen-printed electrodes
OT  - Sulfonamides
OT  - Tetracyclines
EDAT- 2014/04/22 06:00
MHDA- 2015/08/20 06:00
CRDT- 2014/04/22 06:00
PHST- 2014/01/10 00:00 [received]
PHST- 2014/03/04 00:00 [revised]
PHST- 2014/03/07 00:00 [accepted]
PHST- 2014/04/22 06:00 [entrez]
PHST- 2014/04/22 06:00 [pubmed]
PHST- 2015/08/20 06:00 [medline]
AID - S0003-2670(14)00308-0 [pii]
AID - 10.1016/j.aca.2014.03.005 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2014 Apr 11;820:32-8. doi: 10.1016/j.aca.2014.03.005. Epub 2014 
      Mar 12.