PMID- 24746495
OWN - NLM
STAT- MEDLINE
DCOM- 20141215
LR  - 20140516
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1566
DP  - 2014 May 30
TI  - Brain stem slice conditioned medium contains endogenous BDNF and GDNF that affect 
      neural crest boundary cap cells in co-culture.
PG  - 12-23
LID - S0006-8993(14)00483-1 [pii]
LID - 10.1016/j.brainres.2014.04.006 [doi]
AB  - Conditioned medium (CM), made by collecting medium after a few days in cell 
      culture and then re-using it to further stimulate other cells, is a known 
      experimental concept since the 1950s. Our group has explored this technique to 
      stimulate the performance of cells in culture in general, and to evaluate stem- 
      and progenitor cell aptitude for auditory nerve repair enhancement in particular. 
      As compared to other mediums, all primary endpoints in our published experimental 
      settings have weighed in favor of conditioned culture medium, where we have shown 
      that conditioned culture medium has a stimulatory effect on cell survival. In 
      order to explore the reasons for this improved survival we set out to analyze the 
      conditioned culture medium. We utilized ELISA kits to investigate whether brain 
      stem (BS) slice CM contains any significant amounts of brain-derived neurotrophic 
      factor (BDNF) and glial cell derived neurotrophic factor (GDNF). We further 
      looked for a donor cell with progenitor characteristics that would be receptive 
      to BDNF and GDNF. We chose the well-documented boundary cap (BC) progenitor cells 
      to be tested in our in vitro co-culture setting together with cochlear nucleus 
      (CN) of the BS. The results show that BS CM contains BDNF and GDNF and that 
      survival of BC cells, as well as BC cell differentiation into neurons, were 
      enhanced when BS CM were used. Altogether, we conclude that BC cells transplanted 
      into a BDNF and GDNF rich environment could be suitable for treatment of a 
      traumatized or degenerated auditory nerve.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Kaiser, Andreas
AU  - Kaiser A
AD  - Department of Clinical Science, Intervention and Technology, Karolinska 
      University Hospital, 17176 Stockholm, Sweden. Electronic address: 
      andreas.kaiser@karolinska.se.
FAU - Kale, Ajay
AU  - Kale A
AD  - Department of Clinical Science, Intervention and Technology, Karolinska 
      University Hospital, 17176 Stockholm, Sweden.
FAU - Novozhilova, Ekaterina
AU  - Novozhilova E
AD  - Department of Clinical Science, Intervention and Technology, Karolinska 
      University Hospital, 17176 Stockholm, Sweden.
FAU - Siratirakun, Piyaporn
AU  - Siratirakun P
AD  - Department of Clinical Science, Intervention and Technology, Karolinska 
      University Hospital, 17176 Stockholm, Sweden.
FAU - Aquino, Jorge B
AU  - Aquino JB
AD  - Unit of Molecular Neurobiology-MBB, Karolinska Institutet, 17177 Stockholm, 
      Sweden.
FAU - Thonabulsombat, Charoensri
AU  - Thonabulsombat C
AD  - Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, 
      Thailand.
FAU - Ernfors, Patrik
AU  - Ernfors P
AD  - Unit of Molecular Neurobiology-MBB, Karolinska Institutet, 17177 Stockholm, 
      Sweden.
FAU - Olivius, Petri
AU  - Olivius P
AD  - Department of Clinical and Experimental Medicine, Faculty of Health Sciences, 
      58183 Linkoping, Sweden.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140416
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Brain Stem/embryology/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Coculture Techniques
MH  - Culture Media, Conditioned
MH  - Glial Cell Line-Derived Neurotrophic Factor/*metabolism
MH  - Mice
MH  - Neural Crest/metabolism
MH  - Neural Stem Cells/cytology/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - Auditory brainstem slice
OT  - Boundary cap cells
OT  - Hearing
OT  - Neural crest
OT  - Neuronal restoration
OT  - Organotypic culture
EDAT- 2014/04/22 06:00
MHDA- 2014/12/17 06:00
CRDT- 2014/04/22 06:00
PHST- 2013/11/17 00:00 [received]
PHST- 2014/03/17 00:00 [revised]
PHST- 2014/04/07 00:00 [accepted]
PHST- 2014/04/22 06:00 [entrez]
PHST- 2014/04/22 06:00 [pubmed]
PHST- 2014/12/17 06:00 [medline]
AID - S0006-8993(14)00483-1 [pii]
AID - 10.1016/j.brainres.2014.04.006 [doi]
PST - ppublish
SO  - Brain Res. 2014 May 30;1566:12-23. doi: 10.1016/j.brainres.2014.04.006. Epub 2014 
      Apr 16.