PMID- 24747040
OWN - NLM
STAT- MEDLINE
DCOM- 20141223
LR  - 20140513
IS  - 1095-8274 (Electronic)
IS  - 1075-9964 (Linking)
VI  - 27
DP  - 2014 Jun
TI  - Relationship between gastrointestinal dysbiosis and Clostridium botulinum in 
      dairy cows.
PG  - 100-5
LID - S1075-9964(14)00033-X [pii]
LID - 10.1016/j.anaerobe.2014.03.013 [doi]
AB  - The gastrointestinal tract is a balanced ecosystem that can get out of balance 
      and predisposed to clostridial diseases or other pathological conditions. The 
      objective of the present study was to evaluate the gut microbiota in dairy cows 
      suffering from chronic botulism. Cows were investigated for Clostridium (C.) 
      botulinum in faeces and rumen fluids. In order to study the relationship between 
      botulism and gastrointestinal microbiota, faeces and rumen fluid were tested for 
      bacterial composition using conventional microbiological culture techniques and 
      fluorescence in situ hybridization (FISH). Protozoa were analyzed in rumen fluid 
      microscopically. The presence of C. botulinum was associated with specific 
      changes in the faecal microbiota, especially a significant reduction of total 
      aerobic bacteria, total anaerobic bacteria, enterococci, Clostridium perfringens 
      and yeast and fungi. Also C. botulinum positive rumen fluid had significantly 
      more Bacteroides spp., C. histolyticum group, Alfa- proteobacteria, 
      Gammaproteobacteria, and sulfate-reducing bacteria; as well as significantly 
      fewer Euryaracheota, and the protozoa Epidinium spp. Dasytricha spp., 
      Diplodiniinae spp. and Ophryoscolex spp. In conclusion, C. botulinum is common in 
      dairy cows in Germany but the incidence of botulism is associated with microbial 
      changes and composition in the gastrointestinal tract. Bacteria, yeast and 
      protozoa appear to be crucial in the colonization process; however, the 
      chronology of these events and role of each microbial group needs further 
      evaluation.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Kruger, Monika
AU  - Kruger M
AD  - Institute of Bacteriology and Mycology, Faculty of Veterinary Medicine, Leipzig 
      University, An den Tierkliniken 29, 04103 Leipzig, Germany.
FAU - Shehata, Awad A
AU  - Shehata AA
AD  - Institute of Bacteriology and Mycology, Faculty of Veterinary Medicine, Leipzig 
      University, An den Tierkliniken 29, 04103 Leipzig, Germany; Avian and Rabbit 
      Diseases Department, Faculty of Veterinary Medicine, Sadat City University, 
      Egypt. Electronic address: shehata@vetmed.uni-leipzig.de.
FAU - Grosse-Herrenthey, Anke
AU  - Grosse-Herrenthey A
AD  - Institute of Bacteriology and Mycology, Faculty of Veterinary Medicine, Leipzig 
      University, An den Tierkliniken 29, 04103 Leipzig, Germany.
FAU - Stander, Norman
AU  - Stander N
AD  - Institute of Bacteriology and Mycology, Faculty of Veterinary Medicine, Leipzig 
      University, An den Tierkliniken 29, 04103 Leipzig, Germany.
FAU - Schrodl, Wieland
AU  - Schrodl W
AD  - Institute of Bacteriology and Mycology, Faculty of Veterinary Medicine, Leipzig 
      University, An den Tierkliniken 29, 04103 Leipzig, Germany.
LA  - eng
PT  - Journal Article
DEP - 20140416
PL  - England
TA  - Anaerobe
JT  - Anaerobe
JID - 9505216
SB  - IM
MH  - Animals
MH  - *Biota
MH  - Cattle
MH  - Clostridium botulinum/*growth & development
MH  - *Dysbiosis
MH  - Feces/microbiology
MH  - Gastrointestinal Tract/*microbiology
MH  - Germany
MH  - In Situ Hybridization, Fluorescence
MH  - Microbiological Techniques
MH  - Microscopy
MH  - Rumen/microbiology
OTO - NOTNLM
OT  - BoNT
OT  - C. botulinum
OT  - Dysbiosis
OT  - Gut microbiota
OT  - Protozoa
EDAT- 2014/04/22 06:00
MHDA- 2014/12/24 06:00
CRDT- 2014/04/22 06:00
PHST- 2013/12/23 00:00 [received]
PHST- 2014/03/25 00:00 [revised]
PHST- 2014/03/27 00:00 [accepted]
PHST- 2014/04/22 06:00 [entrez]
PHST- 2014/04/22 06:00 [pubmed]
PHST- 2014/12/24 06:00 [medline]
AID - S1075-9964(14)00033-X [pii]
AID - 10.1016/j.anaerobe.2014.03.013 [doi]
PST - ppublish
SO  - Anaerobe. 2014 Jun;27:100-5. doi: 10.1016/j.anaerobe.2014.03.013. Epub 2014 Apr 
      16.