PMID- 24749493
OWN - NLM
STAT- MEDLINE
DCOM- 20150710
LR  - 20191210
IS  - 1460-2202 (Electronic)
IS  - 0271-3683 (Linking)
VI  - 39
IP  - 12
DP  - 2014 Dec
TI  - Functional hierarchy of herpes simplex virus type-1 membrane proteins in corneal 
      infection and virus transmission to ganglionic neurons.
PG  - 1169-77
LID - 10.3109/02713683.2014.906626 [doi]
AB  - PURPOSE: To determine the relative importance of viral glycoproteins gK, gM, gE 
      and the membrane protein UL11 in infection of mouse corneas and ganglionic 
      neurons. METHODS: Mouse eyes were scarified and infected with herpes simplex 
      virus (HSV)-1(F), gE-null, gM-null, gK-null, or UL11-null viruses. Clinical signs 
      of ocular disease were monitored daily. Virus shedding was determined at 24, 48 
      and 72 h post infection. Viral DNA within trigeminal ganglia (TG) was quantified 
      by quantitative PCR at 30 d post infection. RESULTS: The gE-null virus replicated 
      as efficiently as the parental virus and formed viral plaques approximately 
      half-the-size in comparison with the HSV-1(F) wild-type virus. The UL11-null and 
      gM-null viruses replicated approximately one log less efficiently than the 
      wild-type virus, and formed plaques that were on average one-third the size and 
      one-half the size of the wild-type virus, respectively. The gK-null virus 
      replicated more than 3-logs less efficiently than the wild-type virus and formed 
      very small plaques (5-10 cells). Mice infected with the wild-type virus exhibited 
      mild clinical ocular symptoms, while mice infected with the mutant viruses did 
      not show any significant ocular changes. The wild-type virus produced the highest 
      virus shedding post infection followed by the gM-null, gE-null and UL11-null 
      viruses, while no gK-null virus was detected at any time point. All TG collected 
      from mice infected with the wild-type virus and 6-of-10 of TG retrieved from mice 
      infected with the UL11-null virus contained high numbers of viral genomes. The 
      gE-null and gM-null-infected ganglia contained moderate-to-low number of viral 
      genomes in 4-of-10 and 2-of-10 mice, respectively. No viral genomes were detected 
      in ganglionic tissues obtained from gK-null eye infections. CONCLUSIONS: The 
      results show that gK plays the most important role among gM, gE and UL11 in 
      corneal and ganglionic infection in the mouse eye model.
FAU - Kim, In-Joong
AU  - Kim IJ
AD  - Division of Biotechnology & Molecular Medicine, Department of Pathobiological 
      Sciences, School of Veterinary Medicine, Louisiana State University , Baton 
      Rouge, LA , USA.
FAU - Saied, Ahmad A
AU  - Saied AA
FAU - Chouljenko, Vladimir N
AU  - Chouljenko VN
FAU - Subramanian, Ramesh
AU  - Subramanian R
FAU - Kousoulas, Konstantin G
AU  - Kousoulas KG
LA  - eng
GR  - P20GM103458/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140421
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (DNA, Viral)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (UL10 protein, Human herpesvirus 1)
RN  - 0 (UL11 protein, herpesviridae)
RN  - 0 (UL53 protein, Human herpesvirus 1)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (Viral Structural Proteins)
RN  - 0 (glycoprotein E, herpes simplex virus type 1)
SB  - IM
MH  - Animals
MH  - Chlorocebus aethiops
MH  - Chromosomes, Artificial, Bacterial
MH  - Cornea/*innervation/virology
MH  - DNA, Viral/analysis
MH  - Disease Models, Animal
MH  - Herpesvirus 1, Human/*physiology
MH  - Keratitis, Herpetic/*virology
MH  - Membrane Glycoproteins/physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Real-Time Polymerase Chain Reaction
MH  - Trigeminal Ganglion/*virology
MH  - Vero Cells
MH  - Viral Envelope Proteins/physiology
MH  - Viral Matrix Proteins/*physiology
MH  - Viral Proteins/physiology
MH  - Viral Structural Proteins/physiology
MH  - *Virus Replication
MH  - Virus Shedding/physiology
OTO - NOTNLM
OT  - UL11
OT  - gE
OT  - gK
OT  - gM
OT  - herpes simplex virus
OT  - neuroinvasion
OT  - ocular infection
EDAT- 2014/04/23 06:00
MHDA- 2015/07/15 06:00
CRDT- 2014/04/23 06:00
PHST- 2014/04/23 06:00 [entrez]
PHST- 2014/04/23 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - 10.3109/02713683.2014.906626 [doi]
PST - ppublish
SO  - Curr Eye Res. 2014 Dec;39(12):1169-77. doi: 10.3109/02713683.2014.906626. Epub 
      2014 Apr 21.