PMID- 24750267
OWN - NLM
STAT- MEDLINE
DCOM- 20150930
LR  - 20211021
IS  - 1939-1676 (Electronic)
IS  - 0891-6640 (Print)
IS  - 0891-6640 (Linking)
VI  - 28
IP  - 4
DP  - 2014 Jul-Aug
TI  - Effect of dietary nonstructural carbohydrate content on activation of 
      5'-adenosine monophosphate-activated protein kinase in liver, skeletal muscle, 
      and digital laminae of lean and obese ponies.
PG  - 1280-8
LID - 10.1111/jvim.12356 [doi]
AB  - BACKGROUND: In EMS-associated laminitis, laminar failure may occur in response to 
      energy failure related to insulin resistance (IR) or to the effect of 
      hyperinsulinemia on laminar tissue. 5'-Adenosine-monophosphate-activated protein 
      kinase (AMPK) is a marker of tissue energy deprivation, which may occur in IR. 
      HYPOTHESIS/OBJECTIVES: To characterize tissue AMPK regulation in ponies subjected 
      to a dietary carbohydrate (CHO) challenge. ANIMALS: Twenty-two mixed-breed 
      ponies. METHODS: Immunohistochemistry and immunoblotting for total AMPK and 
      phospho(P)-AMPK and RT-qPCR for AMPK-responsive genes were performed on laminar, 
      liver, and skeletal muscle samples collected after a 7-day feeding protocol in 
      which ponies stratified on body condition score (BCS; obese or lean) were fed 
      either a low-CHO diet (ESC + starch, approximately 7% DM; n = 5 obese, 5 lean) or 
      a high-CHO diet (ESC + starch, approximately 42% DM; n = 6 obese, 6 lean). 
      RESULTS: 5'-Adenosine-monophosphate-activated protein kinase was immunolocalized 
      to laminar keratinocytes, dermal constituents, and hepatocytes. A high-CHO diet 
      resulted in significantly decreased laminar [P-AMPK] in lean ponies (P = .03), 
      but no changes in skeletal muscle (lean, P = .33; obese, P = .43) or liver (lean, 
      P = .84; obese, P = .13) [P-AMPK]. An inverse correlation existed between [blood 
      glucose] and laminar [P-AMPK] in obese ponies on a high-CHO diet. CONCLUSIONS AND 
      CLINICAL IMPORTANCE: Laminar tissue exhibited a normal response to a high-CHO 
      diet (decreased [P-AMPK]), whereas this response was not observed in liver and 
      skeletal muscle in both lean (skeletal muscle, P = .33; liver, P = .84) and obese 
      (skeletal muscle, P = .43; liver, P = .13) ponies.
CI  - Copyright (c) 2014 by the American College of Veterinary Internal Medicine.
FAU - Burns, T A
AU  - Burns TA
AD  - The Ohio State University College of Veterinary Medicine, Columbus, OH.
FAU - Watts, M R
AU  - Watts MR
FAU - Weber, P S
AU  - Weber PS
FAU - McCutcheon, L J
AU  - McCutcheon LJ
FAU - Geor, R J
AU  - Geor RJ
FAU - Belknap, J K
AU  - Belknap JK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140420
PL  - United States
TA  - J Vet Intern Med
JT  - Journal of veterinary internal medicine
JID - 8708660
RN  - 0 (Blood Glucose)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Insulin)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases/*metabolism
MH  - Animals
MH  - Blood Glucose/analysis
MH  - Blotting, Western/veterinary
MH  - Dietary Carbohydrates/*pharmacology
MH  - Enzyme Activation/drug effects
MH  - Hoof and Claw/drug effects/*enzymology
MH  - Horse Diseases/*enzymology/metabolism
MH  - Horses
MH  - Insulin/blood
MH  - Liver/drug effects/*enzymology
MH  - Muscle, Skeletal/drug effects/*enzymology
MH  - Obesity/enzymology/metabolism/*veterinary
MH  - Real-Time Polymerase Chain Reaction/veterinary
MH  - Thinness/enzymology/metabolism/*veterinary
PMC - PMC4857936
OTO - NOTNLM
OT  - Energy regulation
OT  - Equine metabolic syndrome
OT  - Insulin resistance
OT  - Laminitis
EDAT- 2014/04/23 06:00
MHDA- 2015/10/01 06:00
PMCR- 2014/07/01
CRDT- 2014/04/23 06:00
PHST- 2013/08/15 00:00 [received]
PHST- 2013/12/13 00:00 [revised]
PHST- 2014/03/05 00:00 [accepted]
PHST- 2014/04/23 06:00 [entrez]
PHST- 2014/04/23 06:00 [pubmed]
PHST- 2015/10/01 06:00 [medline]
PHST- 2014/07/01 00:00 [pmc-release]
AID - JVIM12356 [pii]
AID - 10.1111/jvim.12356 [doi]
PST - ppublish
SO  - J Vet Intern Med. 2014 Jul-Aug;28(4):1280-8. doi: 10.1111/jvim.12356. Epub 2014 
      Apr 20.