PMID- 24752084
OWN - NLM
STAT- MEDLINE
DCOM- 20141222
LR  - 20181202
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 34
IP  - 4
DP  - 2014 Apr
TI  - Human umbilical cord-derived mesenchymal stem cells inhibit proliferation but 
      maintain survival of Jurkat leukemia cells in vitro by activating Notch 
      signaling.
PG  - 441-7
AB  - OBJECTIVE: To investigate the effects of human umbilical cord-derived mesenchymal 
      stem cells (hUC-MSCs) on the proliferation and survival of Jurkat leukemia cells 
      in vitro and explore the possible mechanism. METHODS: Jurkat leukemia cells were 
      co-cultured with hUC-MSCs isolated from human umbilical cord tissues by plastic 
      adherence at a ratio of 10:1. The proliferation and survival of the co-cultured 
      Jurkat cells, separated by immunomagnetic bead cell sorting on day 4, were 
      evaluated by flow cytometry. Western blotting was performed to evaluate the 
      activation of Notch signaling in the co-cultured Jurkat cells. RESULTS: Jurkat 
      leukemia cells co-cultured with hUC-MSCs for 4 days showed a lowered 
      proliferation rate and cell cycle arrest at G0/G1 phase with a reduction in the 
      cell apoptotic rate. Notch signaling pathway was activated in the co-cultured 
      Jurkat cells as evidenced by an increased cellular expression of HES-1. 
      CONCLUSION: Co-culture with hUC-MSCs can inhibit the proliferation of Jurkat 
      leukemia cells in vitro and protect the cells from apoptosis by activating Notch 
      signaling, indicating a potential shielding effect of MSCs on leukemia cells.
FAU - Yuan, Yin
AU  - Yuan Y
AD  - School of Biosciences and Biopharmaceutics, Guangdong Province Key Laboratory for 
      Biotechnology Drug Candidates, Guangdong Pharmaceutical University, Guangzhou 
      510006, China.E-mail: yinyinyuan@126.com.
FAU - Chen, Danliang
AU  - Chen D
FAU - Chen, Xuan
AU  - Chen X
FAU - Shao, Hongwei
AU  - Shao H
FAU - Huang, Shulin
AU  - Huang S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (NOTCH1 protein, human)
RN  - 0 (Receptor, Notch1)
SB  - IM
MH  - Apoptosis
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Humans
MH  - Jurkat Cells
MH  - Mesenchymal Stem Cells/*cytology
MH  - Receptor, Notch1/*metabolism
MH  - *Signal Transduction
MH  - Umbilical Cord/*cytology
EDAT- 2014/04/23 06:00
MHDA- 2014/12/23 06:00
CRDT- 2014/04/23 06:00
PHST- 2014/04/23 06:00 [entrez]
PHST- 2014/04/23 06:00 [pubmed]
PHST- 2014/12/23 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2014 Apr;34(4):441-7.