PMID- 24752512
OWN - NLM
STAT- MEDLINE
DCOM- 20150120
LR  - 20140925
IS  - 1097-4598 (Electronic)
IS  - 0148-639X (Linking)
VI  - 50
IP  - 4
DP  - 2014 Oct
TI  - Cytoplasmic 5'-nucleotidase autoantibodies in inclusion body myositis: Isotypes 
      and diagnostic utility.
PG  - 488-92
LID - 10.1002/mus.24199 [doi]
AB  - INTRODUCTION: Recent studies have identified circulating immunoglobulin (Ig) G 
      autoantibodies against cytoplasmic 5'-nucleotidase 1A (cN1A; NT5C1A) in patients 
      with inclusion body myositis (IBM), whose detection provides for an IBM blood 
      diagnostic test. Whether or not anti-cN1A autoantibody isotypes other than IgG 
      are present in IBM has not previously been reported. METHODS: Plasma and serum 
      samples from 205 patients (50 with and155 without IBM) were studied for the 
      presence of IgM and IgA, in addition to IgG, anti-cN1A autoantibodies using 
      immunoblots and enzyme-linked immunoassays (ELISAs). RESULTS: IgM, IgA, and IgG 
      anti-cN1A autoantibodies were detected by ELISA with similar sensitivities 
      (49-53%) and specificities (94-96%), but with differing patterns of autoantibody 
      isotype presence. Combination assays of all 3 autoantibody levels improved 
      diagnostic sensitivity to 76%. CONCLUSIONS: In addition to previously recognized 
      IgG anti-cN1A autoantibodies, IBM patients have circulating IgM and IgA anti-cN1A 
      autoantibodies. Differing patterns of these isotypes may be present and useful 
      for diagnosis.
CI  - Copyright (c) 2014 Wiley Periodicals, Inc.
FAU - Greenberg, Steven A
AU  - Greenberg SA
AD  - Department of Neurology, Brigham and Women's Hospital and Children's Hospital 
      Informatics Program, Harvard Medical School and Harvard-MIT Division of Health 
      Sciences and Technology, Boston, Massachusetts, USA.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Muscle Nerve
JT  - Muscle & nerve
JID - 7803146
RN  - 0 (Autoantibodies)
RN  - 0 (Immunoglobulin Isotypes)
RN  - EC 3.1.3.5 (5'-Nucleotidase)
SB  - IM
MH  - 5'-Nucleotidase/*immunology
MH  - Autoantibodies/*blood
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Immunoglobulin Isotypes/*blood
MH  - Male
MH  - Molecular Weight
MH  - Myositis, Inclusion Body/*blood
MH  - ROC Curve
OTO - NOTNLM
OT  - anti-cN1A antibodies
OT  - inclusion body myositis
EDAT- 2014/04/23 06:00
MHDA- 2015/01/21 06:00
CRDT- 2014/04/23 06:00
PHST- 2013/11/06 00:00 [received]
PHST- 2014/01/30 00:00 [revised]
PHST- 2014/02/04 00:00 [accepted]
PHST- 2014/04/23 06:00 [entrez]
PHST- 2014/04/23 06:00 [pubmed]
PHST- 2015/01/21 06:00 [medline]
AID - 10.1002/mus.24199 [doi]
PST - ppublish
SO  - Muscle Nerve. 2014 Oct;50(4):488-92. doi: 10.1002/mus.24199.