PMID- 2475254
OWN - NLM
STAT- MEDLINE
DCOM- 19890929
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 58
IP  - 4
DP  - 1989 Aug 25
TI  - Secreted form of amyloid beta protein precursor is involved in the growth 
      regulation of fibroblasts.
PG  - 615-22
AB  - Fibroblasts that harbor an antisense construct of amyloid beta protein precursor 
      (ABPP) cDNA, A-1, produced less ABPP mRNA and ABPP and grew poorly. Normal growth 
      was restored when either parent cell conditioned medium (CM) or purified ABPP was 
      provided. The capacity of the CM to restore cell growth was abolished by passage 
      through an anti-ABPP immunoaffinity column; the activity was in the bound 
      fraction. A Mr 90,000 protein recognized by the anti-ABPP antibody was diminished 
      in the CM of A-1. CM from ABPP cDNA-transfected cells expressing high levels of 
      ABPP was more potent than that from non-transfected parent cells in restoring A-1 
      growth. These results indicate that ABPP is released from cells into the medium 
      and has an autocrine function in growth regulation.
FAU - Saitoh, T
AU  - Saitoh T
AD  - Department of Neurosciences, School of Medicine, University of California at San 
      Diego, La Jolla 92093.
FAU - Sundsmo, M
AU  - Sundsmo M
FAU - Roch, J M
AU  - Roch JM
FAU - Kimura, N
AU  - Kimura N
FAU - Cole, G
AU  - Cole G
FAU - Schubert, D
AU  - Schubert D
FAU - Oltersdorf, T
AU  - Oltersdorf T
FAU - Schenk, D B
AU  - Schenk DB
LA  - eng
GR  - AG05131/AG/NIA NIH HHS/United States
GR  - AG08205/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Amyloid)
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Growth Substances)
RN  - 0 (Protein Precursors)
RN  - 0 (RNA, Antisense)
RN  - 0 (RNA, Messenger)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Amyloid/genetics/metabolism/*physiology
MH  - Amyloid beta-Protein Precursor
MH  - Brain/physiology
MH  - Cell Compartmentation
MH  - *Cell Division
MH  - Cell Line
MH  - Fibroblasts/*cytology
MH  - Growth Substances/*physiology
MH  - Humans
MH  - In Vitro Techniques
MH  - Protein Precursors/genetics/metabolism/*physiology
MH  - RNA
MH  - RNA, Antisense
MH  - RNA, Messenger/genetics
MH  - Transfection
EDAT- 1989/08/25 00:00
MHDA- 1989/08/25 00:01
CRDT- 1989/08/25 00:00
PHST- 1989/08/25 00:00 [pubmed]
PHST- 1989/08/25 00:01 [medline]
PHST- 1989/08/25 00:00 [entrez]
AID - 0092-8674(89)90096-2 [pii]
AID - 10.1016/0092-8674(89)90096-2 [doi]
PST - ppublish
SO  - Cell. 1989 Aug 25;58(4):615-22. doi: 10.1016/0092-8674(89)90096-2.