PMID- 24753317 OWN - NLM STAT- MEDLINE DCOM- 20151019 LR - 20220310 IS - 1097-0029 (Electronic) IS - 1059-910X (Linking) VI - 77 IP - 6 DP - 2014 Jun TI - Penetrability of AH plus and MTA fillapex after endodontic treatment and retreatment: a confocal laser scanning microscopy study. PG - 467-71 LID - 10.1002/jemt.22371 [doi] AB - The aim of the study was to assess the penetrability of two endodontic sealers (AH Plus and MTA Fillapex) into dentinal tubules, submitted to endodontic treatment and subsequently to endodontic retreatment. Thirty ex vivo incisors were prepared using ProTaper rotary system up to F3 instrument and divided in three groups according to the endodontic sealer used for root canal filling: AH Plus (AHP), MTA Fillapex (MTAF), and control group (CG) without using EDTA previously to the root canal filling. Rhodamine B dye (red) was incorporated to the sealers in order to provide the fluorescence which will enable confocal laser scanning microscopy (CLSM) assessment. All specimens were filled with gutta-percha cones using the lateral compaction technique. The specimens were submitted to endodontic retreatment using ProTaper Retreatment system, re-prepared up to F5 instruments and filled with gutta-percha cones and the same sealer used during endodontic retreatment. Fluorescein dye (green) was incorporated to the sealer in order to distinguish from the first filling. The roots were sectioned 2 mm from the apex and assessed by CLSM. No difference was found between the two experimental groups (P > 0.05). On the other hand, in the control group the sealers were not capable to penetrate into dentinal tubules after endodontic treatment (P > 0.05). In retreatment cases, none of the sealers were able to penetrate into dentin tubules. It can be concluded that sealer penetrability is high during endodontic treatment. However, MTA Fillapex and AH Plus do not penetrate into dentinal tubules after endodontic retreatment. CI - (c) 2014 Wiley Periodicals, Inc. FAU - Kok, Daniela AU - Kok D AD - Private Practioner, Porto Alegre, RS, Brazil. FAU - Rosa, Ricardo Abreu da AU - Rosa RA FAU - Barreto, Mirela Sangoi AU - Barreto MS FAU - Busanello, Fernanda Hoffmann AU - Busanello FH FAU - Santini, Manuela Favarin AU - Santini MF FAU - Pereira, Jefferson Ricardo AU - Pereira JR FAU - So, Marcus Vinicius Reis AU - So MV LA - eng PT - Journal Article DEP - 20140421 PL - United States TA - Microsc Res Tech JT - Microscopy research and technique JID - 9203012 RN - 0 (Aluminum Compounds) RN - 0 (Calcium Compounds) RN - 0 (Drug Combinations) RN - 0 (Epoxy Resins) RN - 0 (Oxides) RN - 0 (Root Canal Filling Materials) RN - 0 (Silicates) RN - 0 (epoxy resin-based root canal sealer) RN - 0 (mineral trioxide aggregate) SB - IM MH - Aluminum Compounds/*therapeutic use MH - Calcium Compounds/*therapeutic use MH - Drug Combinations MH - Epoxy Resins/*therapeutic use MH - Humans MH - Microscopy, Confocal MH - Oxides/*therapeutic use MH - Retreatment/methods MH - Root Canal Filling Materials/*therapeutic use MH - Root Canal Therapy/*methods MH - Silicates/*therapeutic use MH - Treatment Outcome OTO - NOTNLM OT - confocal laser scanning microscopy OT - endodontics OT - retreatment OT - root canal filling EDAT- 2014/04/23 06:00 MHDA- 2015/10/20 06:00 CRDT- 2014/04/23 06:00 PHST- 2014/02/11 00:00 [received] PHST- 2014/03/31 00:00 [revised] PHST- 2014/04/04 00:00 [accepted] PHST- 2014/04/23 06:00 [entrez] PHST- 2014/04/23 06:00 [pubmed] PHST- 2015/10/20 06:00 [medline] AID - 10.1002/jemt.22371 [doi] PST - ppublish SO - Microsc Res Tech. 2014 Jun;77(6):467-71. doi: 10.1002/jemt.22371. Epub 2014 Apr 21.