PMID- 24753508
OWN - NLM
STAT- MEDLINE
DCOM- 20141007
LR  - 20140815
IS  - 1934-6638 (Electronic)
IS  - 1934-662X (Linking)
VI  - 122
IP  - 8
DP  - 2014 Aug
TI  - Targeted multiprobe fluorescence in situ hybridization analysis for elucidation 
      of inconclusive pancreatobiliary cytology.
PG  - 627-34
LID - 10.1002/cncy.21429 [doi]
AB  - BACKGROUND: Endoscopic fine-needle aspiration (FNA) and brush cytology are 
      standard methods for the diagnosis of pancreatobiliary malignancies. Although the 
      majority of cytological diagnoses are straightforward, there remains a difficult 
      category of inconclusive cytology. This study explored the utility of 
      fluorescence in situ hybridization (FISH) to improve the diagnostic 
      stratification between reactive and malignant cells in cases of inconclusive 
      cytology. METHODS: The multiprobe FISH assay UroVysion was used for copy number 
      assessment of chromosomes 3, 7, 17, and the 9p21 locus on Papanicolaou-stained 
      specimens with a diagnosis of inconclusive cytology (n = 50), adenocarcinoma 
      (n = 31) and no evidence of malignancy (n = 9). The target cells were 
      photographed and their coordinates saved on an automated stage prior to 
      hybridization. A positive test was defined as increased copy number (> 2) of at 
      least 2 chromosomes (3, 7, or 17) in at least 4 atypical cells, or loss of 9p21 
      in at least 12 cells. RESULTS: FISH confirmed all 31 cytological diagnoses of 
      pancreatobiliary adenocarcinomas, and was negative in the 9 patients with 
      negative cytology. Among the 50 cases with inconclusive cytology, FISH detected 
      19 of 31 cases with a final diagnosis of adenocarcinoma, and was negative in all 
      19 cases with no final evidence of malignancy (sensitivity of 61.3%, specificity 
      of 100%, positive predictive value of 100%, negative predictive value of 61.3%). 
      Loss of 9p21 was found in 43 (86%) of all 50 FISH-positive cases. CONCLUSIONS: 
      Multiprobe FISH combined with automated relocation of atypical cells is a 
      powerful technique to clarify inconclusive cytology of the pancreatobiliary 
      tract, allowing for a better distinction between reactive atypia and malignancy.
CI  - (c) 2014 American Cancer Society.
FAU - Vlajnic, Tatjana
AU  - Vlajnic T
AD  - Institute of Pathology, University Hospital Basel, Basel, Switzerland.
FAU - Somaini, Gina
AU  - Somaini G
FAU - Savic, Spasenija
AU  - Savic S
FAU - Barascud, Audrey
AU  - Barascud A
FAU - Grilli, Bruno
AU  - Grilli B
FAU - Herzog, Michelle
AU  - Herzog M
FAU - Obermann, Ellen C
AU  - Obermann EC
FAU - Holmes, Brittany J
AU  - Holmes BJ
FAU - Ali, Syed Z
AU  - Ali SZ
FAU - Degen, Lukas
AU  - Degen L
FAU - Bubendorf, Lukas
AU  - Bubendorf L
LA  - eng
PT  - Journal Article
DEP - 20140418
PL  - United States
TA  - Cancer Cytopathol
JT  - Cancer cytopathology
JID - 101499453
SB  - IM
MH  - Adenocarcinoma/*diagnosis
MH  - Bile Duct Neoplasms/*diagnosis
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Pancreatic Neoplasms/*diagnosis
MH  - Predictive Value of Tests
OTO - NOTNLM
OT  - FISH
OT  - atypia
OT  - biliary tract
OT  - cytology
OT  - fine-needle aspiration
OT  - fluorescence in situ hybridization
OT  - pancreatic cancer
EDAT- 2014/04/23 06:00
MHDA- 2014/10/08 06:00
CRDT- 2014/04/23 06:00
PHST- 2013/12/23 00:00 [received]
PHST- 2014/03/17 00:00 [revised]
PHST- 2014/03/17 00:00 [accepted]
PHST- 2014/04/23 06:00 [entrez]
PHST- 2014/04/23 06:00 [pubmed]
PHST- 2014/10/08 06:00 [medline]
AID - 10.1002/cncy.21429 [doi]
PST - ppublish
SO  - Cancer Cytopathol. 2014 Aug;122(8):627-34. doi: 10.1002/cncy.21429. Epub 2014 Apr 
      18.