PMID- 24758088
OWN - NLM
STAT- MEDLINE
DCOM- 20150105
LR  - 20161018
IS  - 1003-5370 (Print)
IS  - 1003-5370 (Linking)
VI  - 34
IP  - 3
DP  - 2014 Mar
TI  - [Effect of Buyang Huanwu Decoction and its disassembled recipes on rats' 
      neurogenesis after focal cerebral ischemia].
PG  - 342-7
AB  - OBJECTIVE: To explore the effect of Buyang Huanwu Decoction (BYHWD) and its 
      disassembled recipes on rats' neurogenesis after focal cerebral ischemia and to 
      investigate its underlying molecular mechanisms. METHODS: Focal cerebral ischemia 
      model was induced by occlusion of the right middle cerebral artery for 90 min 
      using the intraluminal filament model. Rats were divided into the sham-operation 
      group, the model group, the BYHWD group, the qi supplementing group, and the 
      blood activating group. Medication was performed by gastrogavage 24 h after 
      ischemia for 14 successive days. 5-bromodeoxyuridine (BrdU) (at 50 mg/kg) was 
      intraperitoneally injected, once per day for 14 successive days. The neurological 
      function was assessed using modified neurological severity score (mNSS) and the 
      corner test at day 1, 7, and 14 after ischemia. BrdU/Nestin, BrdU/NeuN, and 
      BrdU/GFAP positive cells were examined by double immunofluorescence at day 14 
      after ischemia. The protein expression of brain-derived neurotrophic factor 
      (BDNF) and vascular endothelial growth factor (VEGF) were detected by Western 
      blot at day 14 after ischemia. RESULTS: Compared with the model group, the score 
      of mNSS and the frequency of turning right significantly decreased in the BYHWD 
      group and the qi supplementing group (P < 0.01), the number of BrdU/Nestin in the 
      subventricular zone of the lateral ventricle, and BrdU/ NeuN and BrdU/GFAP 
      positive cells in the peripheral ischemic cortex increased (P < 0.05, P < 0.01), 
      protein expression of BDNF and VEGF increased (P < 0.05, P < 0.01). In the qi 
      supplementing group, there was no statistical difference in BrdU/GFAP. But there 
      was no statistical difference in each index of the blood activating group (P > 
      0.05). Compared with BYHWD group, the number of BrdU/Nestin, BrdU/ NeuN, and 
      BrdU/GFAP positive cells significantly decreased (P < 0.01), and the protein 
      expression of BDNF and VEGF were significantly reduced in the qi supplementing 
      group and the blood activating group (P < 0.01). CONCLUSIONS: BYHWD could 
      significantly improve neurogenesis and neurological function recovery after focal 
      cerebral ischemia in rats. Its mechanisms might be related to up-regulating 
      protein expression of BDNF and VEGF. Drugs for qi supplementing and drugs for 
      blood activating had synergistic effects.
FAU - Qu, Tie-Binq
AU  - Qu TB
FAU - Yu, Tian-Hon
AU  - Yu TH
FAU - Liu, Zhi-Ting
AU  - Liu ZT
FAU - Li, Lin
AU  - Li L
FAU - Chu, Li-Sheng
AU  - Chu LS
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Zhong Xi Yi Jie He Za Zhi
JT  - Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese 
      journal of integrated traditional and Western medicine
JID - 9211576
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (buyang huanwu)
RN  - 0 (vascular endothelial growth factor A, rat)
SB  - IM
MH  - Animals
MH  - Brain Ischemia/drug therapy/*metabolism
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Drugs, Chinese Herbal/*pharmacology/therapeutic use
MH  - Male
MH  - Neurogenesis/*drug effects
MH  - Phytotherapy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Vascular Endothelial Growth Factor A/metabolism
EDAT- 2014/04/25 06:00
MHDA- 2015/01/06 06:00
CRDT- 2014/04/25 06:00
PHST- 2014/04/25 06:00 [entrez]
PHST- 2014/04/25 06:00 [pubmed]
PHST- 2015/01/06 06:00 [medline]
PST - ppublish
SO  - Zhongguo Zhong Xi Yi Jie He Za Zhi. 2014 Mar;34(3):342-7.