PMID- 24758738 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20150415 LR - 20140520 IS - 1520-5827 (Electronic) IS - 0743-7463 (Linking) VI - 30 IP - 19 DP - 2014 May 20 TI - From bipolar to quadrupolar electrode structures: an application of bond-detach lithography for dielectrophoretic particle assembly. PG - 5686-93 LID - 10.1021/la5005193 [doi] AB - We describe a new, simple process for fabricating transparent quadrupolar electrode arrays enabling large-scale particle assembly by means of dielectrophoresis. In the first step, interdigitated electrode arrays are made by chemical wet etching of indium tin oxide (ITO). Then, the transition from a bipolar to a quadrupolar electrode arrangement is obtained by covering the electrode surface with a thin poly(dimethylsiloxane) (PDMS) film acting as an electrical insulation layer in which selective openings are formed using bond-detach lithography. The PDMS insulating layer thickness was optimized and controlled by adjusting experimental parameters such as the PDMS viscosity (modulated by the addition of heptane) and the PDMS spin-coating velocity. The insulating character of the PDMS membrane was successfully demonstrated by performing a dielectrophoretic assembly of polystyrene particles using interdigitated electrodes with and without a PDMS layer. The results show that the patterned PDMS film functions properly as an electrical insulation layer and allows the reconfiguration of the electric field cartography. Electric field simulations were performed in both configurations to predict the dielectrophoretic behavior of the particles. The simulation results are in perfect agreement with experiments, in which we demonstrated the formation of concentrated clusters of polystyrene particles and living cells of regular size and shape. FAU - Menad, Samia AU - Menad S AD - Universite de Lyon , Ecole Centrale de Lyon, CNRS, UMR 5005, Laboratoire Ampere, Ecully, F-69130, France. FAU - El-Gaddar, Amal AU - El-Gaddar A FAU - Haddour, Naoufel AU - Haddour N FAU - Toru, Sylvain AU - Toru S FAU - Brun, Mathieu AU - Brun M FAU - Buret, Francois AU - Buret F FAU - Frenea-Robin, Marie AU - Frenea-Robin M LA - eng PT - Journal Article DEP - 20140505 PL - United States TA - Langmuir JT - Langmuir : the ACS journal of surfaces and colloids JID - 9882736 EDAT- 2014/04/25 06:00 MHDA- 2014/04/25 06:01 CRDT- 2014/04/25 06:00 PHST- 2014/04/25 06:00 [entrez] PHST- 2014/04/25 06:00 [pubmed] PHST- 2014/04/25 06:01 [medline] AID - 10.1021/la5005193 [doi] PST - ppublish SO - Langmuir. 2014 May 20;30(19):5686-93. doi: 10.1021/la5005193. Epub 2014 May 5.