PMID- 24760979
OWN - NLM
STAT- MEDLINE
DCOM- 20140818
LR  - 20220408
IS  - 1522-1563 (Electronic)
IS  - 0363-6143 (Linking)
VI  - 306
IP  - 12
DP  - 2014 Jun 15
TI  - The PTEN/PI3K/Akt signaling pathway mediates HMGB1-induced cell proliferation by 
      regulating the NF-kappaB/cyclin D1 pathway in mouse mesangial cells.
PG  - C1119-28
LID - 10.1152/ajpcell.00385.2013 [doi]
AB  - Our previous experiment confirmed that high-mobility group box chromosomal 
      protein 1 (HMGB1) was involved in the pathogenesis of Lupus nephritis (LN) by 
      upregulating the proliferation of the mouse mesangial cell line (MMC) through the 
      cyclin D1/CDK4/p16 system, but the precise mechanism is still unknown. Therefore, 
      in the present study, we demonstrated that HMGB1 induced the proliferation of MMC 
      cells in a time- and concentration-dependent manner, downregulated phosphatase 
      and tensin homolog deleted on chromosome ten (PTEN) expression, increased the 
      level of Akt serine 473 phosphorylation, and induced p65 subunit nuclear 
      translocation. The overexpression of PTEN prevented the upregulation of 
      HMGB1-induced proliferation by blocking the activation of Akt. The knockdown of 
      Akt by siRNA technology and blocking the nuclear factor-kappaB (NF-kappaB) pathway using 
      pyrrolidine dithiocarbamate (PDTC) and SN50, inhibitors of NF-kappaB, both attenuated 
      the HMGB1-induced proliferation by counteracting the activation of the cyclin D1. 
      In addition, while sh-Akt partly blocked the nuclear translocation of the p65 
      subunit, PDTC did not affect the activation of the Akt induced by HMGB1 in MMC 
      cells. These findings indicate that HMGB1 induced the proliferation of MMC cells 
      by activating the PTEN/phosphoinositide-3-kinase (PI3K)/Akt/NF-kappaB signaling 
      pathway.
CI  - Copyright (c) 2014 the American Physiological Society.
FAU - Feng, Xiao-Juan
AU  - Feng XJ
AD  - Department of Pathology, Hebei Medical University, Key Laboratory of Kidney 
      Diseases of Hebei Province, Shijiazhuang, China; and.
FAU - Liu, Shu-Xia
AU  - Liu SX
AD  - Department of Pathology, Hebei Medical University, Key Laboratory of Kidney 
      Diseases of Hebei Province, Shijiazhuang, China; and.
FAU - Wu, Chao
AU  - Wu C
AD  - Department of Pathology, Hebei Medical University, Key Laboratory of Kidney 
      Diseases of Hebei Province, Shijiazhuang, China; and.
FAU - Kang, Peng-Peng
AU  - Kang PP
AD  - Department of Pathology, Hebei Medical University, Key Laboratory of Kidney 
      Diseases of Hebei Province, Shijiazhuang, China; and.
FAU - Liu, Qing-Juan
AU  - Liu QJ
AD  - Department of Pathology, Hebei Medical University, Key Laboratory of Kidney 
      Diseases of Hebei Province, Shijiazhuang, China; and.
FAU - Hao, Jun
AU  - Hao J
AD  - Department of Pathology, Hebei Medical University, Key Laboratory of Kidney 
      Diseases of Hebei Province, Shijiazhuang, China; and.
FAU - Li, Hong-Bo
AU  - Li HB
AD  - Department of Pathology, Hebei Medical University, Key Laboratory of Kidney 
      Diseases of Hebei Province, Shijiazhuang, China; and.
FAU - Li, Fan
AU  - Li F
AD  - Department of Pathology, Hebei Medical University, Key Laboratory of Kidney 
      Diseases of Hebei Province, Shijiazhuang, China; and.
FAU - Zhang, Yu-Jun
AU  - Zhang YJ
AD  - Department of Pathology, Hebei Medical University, Key Laboratory of Kidney 
      Diseases of Hebei Province, Shijiazhuang, China; and.
FAU - Fu, Xiao-Hui
AU  - Fu XH
AD  - Department of Pathology, Hebei Medical University, Key Laboratory of Kidney 
      Diseases of Hebei Province, Shijiazhuang, China; and.
FAU - Zhang, San-Bing
AU  - Zhang SB
AD  - Department of Hand and Foot Surgery, Third Hospital of Shijiazhuang City, 
      Shijiazhuang, China.
FAU - Zuo, Lian-Fu
AU  - Zuo LF
AD  - Department of Pathology, Hebei Medical University, Key Laboratory of Kidney 
      Diseases of Hebei Province, Shijiazhuang, China; and zlf9205@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140423
PL  - United States
TA  - Am J Physiol Cell Physiol
JT  - American journal of physiology. Cell physiology
JID - 100901225
RN  - 0 (HMGB1 Protein)
RN  - 0 (HMGB1 protein, mouse)
RN  - 0 (NF-kappa B)
RN  - 136601-57-5 (Cyclin D1)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Oncogene Protein v-akt)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
RN  - EC 3.1.3.67 (Pten protein, mouse)
SB  - IM
MH  - Animals
MH  - Cell Proliferation
MH  - Cyclin D1/*genetics/metabolism
MH  - Gene Knockdown Techniques
MH  - HMGB1 Protein/*genetics/metabolism
MH  - Lupus Nephritis/*genetics/metabolism/pathology
MH  - Mesangial Cells/*metabolism
MH  - Mice
MH  - NF-kappa B/genetics/metabolism
MH  - Oncogene Protein v-akt/genetics/metabolism
MH  - PTEN Phosphohydrolase/*genetics/metabolism
MH  - Phosphatidylinositol 3-Kinases/genetics
MH  - Phosphorylation
MH  - Signal Transduction/genetics
OTO - NOTNLM
OT  - Akt
OT  - HMGB1
OT  - NF-kappaB
OT  - PTEN
OT  - cell proliferation
OT  - mouse mesangial cell
EDAT- 2014/04/25 06:00
MHDA- 2014/08/19 06:00
CRDT- 2014/04/25 06:00
PHST- 2014/04/25 06:00 [entrez]
PHST- 2014/04/25 06:00 [pubmed]
PHST- 2014/08/19 06:00 [medline]
AID - ajpcell.00385.2013 [pii]
AID - 10.1152/ajpcell.00385.2013 [doi]
PST - ppublish
SO  - Am J Physiol Cell Physiol. 2014 Jun 15;306(12):C1119-28. doi: 
      10.1152/ajpcell.00385.2013. Epub 2014 Apr 23.