PMID- 24765180
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 7
IP  - 5
DP  - 2014 May
TI  - Effect of the LPA-mediated CXCL12-CXCR4 axis in the tumor proliferation, 
      migration and invasion of ovarian cancer cell lines.
PG  - 1581-1585
AB  - Ovarian cancer is the most fatal gynecological cancer, with a 5-year survival 
      rate of only 30%. Lysophosphatidic acid (LPA), which possesses growth factor-like 
      functions, is a major regulatory factor in the peritoneal metastasis of ovarian 
      cancer. LPA stimulates the expression of numerous genes that are associated with 
      angiogenesis and metastasis. Ovarian epithelial carcinoma specifically expresses 
      chemotactic factor C-X-C motif chemokine ligand 12 (CXCL12) and its receptor, CXC 
      receptor 4 (CXCR4). The CXCL12-CXCR4 axis directly contributes to ovarian cancer 
      cell proliferation, migration and invasion. The present study investigated the 
      regulation of LPA on the CXCL12-CXCR4 axis and the effect of the LPA-mediated 
      CXCL12-CXCR4 axis on the tumor proliferation, migration and invasion of ovarian 
      cancer cell lines. The CXCR4 proteins expressed in the cell membrane and the 
      cytoplasm of ovarian cancer cells, CAOV3 and SKOV3, were detected by 
      immunocytochemistry. The expression of CXCR4 and CXCL12 was increased in the 
      ovarian cancer cells in a dose- and time-dependent manner when treated with LPA 
      compared with the control groups (P<0.05), as determined by reverse transcription 
      polymerase chain reaction and flow cytometry. LPA (20 muM) and CXCL12 (100 ng/ml) 
      enhanced the proliferation, migration and invasion of the ovarian cancer cells, 
      CAOV3 and SKOV3, as identified by MTT, Transwell and Matrigel assays following 
      co-treatment for 24 h. LPA promoted invasiveness of ovarian cancer by 
      upregulating CXCL12-CXCR4 axis expression.
FAU - Wang, Hui
AU  - Wang H
AD  - Department of Obstetrics and Gynaecology, General Hospital of PLA, Beijing 
      100853, P.R. China ; Department of Obstetrics and Gynaecology, Shijingshan 
      Teaching Hospital of Capital Medical University, Beijing Shijingshan Hospital, 
      Beijing 100043, P.R. China.
FAU - Liu, Wenli
AU  - Liu W
AD  - Affiliated Hospital of Hebei University of Engineering, Handan, Hebei 056002, 
      P.R. China.
FAU - Wei, Dongmin
AU  - Wei D
AD  - Affiliated Hospital of Hebei University of Engineering, Handan, Hebei 056002, 
      P.R. China.
FAU - Hu, Kun
AU  - Hu K
AD  - Department of Obstetrics and Gynaecology, Shijingshan Teaching Hospital of 
      Capital Medical University, Beijing Shijingshan Hospital, Beijing 100043, P.R. 
      China.
FAU - Wu, Xiaohua
AU  - Wu X
AD  - Department of Obstetrics and Gynaecology, Bethune International Peace Hospital of 
      PLA, Shijiazhuang, Hebei 050000, P.R. China.
FAU - Yao, Yuanqing
AU  - Yao Y
AD  - Department of Obstetrics and Gynaecology, General Hospital of PLA, Beijing 
      100853, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20140228
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC3997722
OTO - NOTNLM
OT  - CXCL12-CXCR4 axis
OT  - lysophosphatidic acid
OT  - metastasis
OT  - ovarian neoplasm
EDAT- 2014/04/26 06:00
MHDA- 2014/04/26 06:01
PMCR- 2014/02/28
CRDT- 2014/04/26 06:00
PHST- 2013/08/04 00:00 [received]
PHST- 2014/01/27 00:00 [accepted]
PHST- 2014/04/26 06:00 [entrez]
PHST- 2014/04/26 06:00 [pubmed]
PHST- 2014/04/26 06:01 [medline]
PHST- 2014/02/28 00:00 [pmc-release]
AID - ol-07-05-1581 [pii]
AID - 10.3892/ol.2014.1926 [doi]
PST - ppublish
SO  - Oncol Lett. 2014 May;7(5):1581-1585. doi: 10.3892/ol.2014.1926. Epub 2014 Feb 28.