PMID- 24765659
OWN - NLM
STAT- MEDLINE
DCOM- 20140623
LR  - 20221022
IS  - 1365-294X (Electronic)
IS  - 0962-1083 (Linking)
VI  - 23
IP  - 6
DP  - 2014 Mar
TI  - Hydrocarbon contamination and plant species determine the phylogenetic and 
      functional diversity of endophytic degrading bacteria.
PG  - 1392-1404
LID - 10.1111/mec.12559 [doi]
AB  - Salt marsh sediments are sinks for various anthropogenic contaminants, giving 
      rise to significant environmental concern. The process of salt marsh plant 
      survival in such environment is very intriguing and at the same time poorly 
      understood. The plant-microbe interactions may play a key role in the process of 
      environment and in planta detoxification.In this study, a combination of 
      culture-dependent and culture-independent molecular approaches [enrichment 
      cultures, polymerase chain reaction (PCR), denaturing gradient gel 
      electrophoresis (DGGE), DNA sequencing] were used to investigate the effect of 
      petroleum hydrocarbons (PH) contamination on the structure and 
      function[polycyclic aromatic hydrocarbon (PAH) dioxygenase genes] of endophytic 
      bacterial communities of salt marsh plant species (Halimione portulacoides and 
      Sarcocornia perennis)in the estuarine system Ria de Aveiro (Portugal). 
      Pseudomonads dominated the cultivable fraction of the endophytic communities in 
      the enrichment cultures. In a set of fifty isolates tested, nine were positive 
      for genes encoding for PAH dioxygenases (nahAc)and four were positive for plasmid 
      carrying genes encoding PAH degradation enzymes(nahAc). Interestingly, these 
      plasmids were only detected in isolates from most severely PH-polluted sites. The 
      results revealed site-specific effects on endophytic communities,related to the 
      level of PH contamination in the sediment, and plant-species-specific 'imprints' 
      in community structure and in genes encoding for PAH dioxygenases. These results 
      suggest a potential ecological role of bacterial plant symbiosis in the process 
      of plant colonization in urban estuarine areas exposed to PH contamination.
FAU - Oliveira, Vanessa
AU  - Oliveira V
AD  - Department of Biology & Center for Environmental and Marine Studies (CESAM), 
      University of Aveiro, Campus Universitario de Santiago, 3810-193, Aveiro, 
      Portugal.
FAU - Gomes, Newton C M
AU  - Gomes NCM
AD  - Department of Biology & Center for Environmental and Marine Studies (CESAM), 
      University of Aveiro, Campus Universitario de Santiago, 3810-193, Aveiro, 
      Portugal.
FAU - Almeida, Adelaide
AU  - Almeida A
AD  - Department of Biology & Center for Environmental and Marine Studies (CESAM), 
      University of Aveiro, Campus Universitario de Santiago, 3810-193, Aveiro, 
      Portugal.
FAU - Silva, Artur M S
AU  - Silva AMS
AD  - Department of Chemistry & QOPNA, University of Aveiro, Campus Universitario de 
      Santiago, 3810-193, Aveiro, Portugal.
FAU - Simoes, Mario M Q
AU  - Simoes MMQ
AD  - Department of Chemistry & QOPNA, University of Aveiro, Campus Universitario de 
      Santiago, 3810-193, Aveiro, Portugal.
FAU - Smalla, Kornelia
AU  - Smalla K
AD  - Julius Kuhn-Institut - Federal Research Centre for Cultivated Plants (JKI), 
      Department of Epidemiology and Pathogen Diagnostics, Messeweg 11-12, 38104, 
      Braunschweig, Germany.
FAU - Cunha, Angela
AU  - Cunha A
AD  - Department of Biology & Center for Environmental and Marine Studies (CESAM), 
      University of Aveiro, Campus Universitario de Santiago, 3810-193, Aveiro, 
      Portugal.
LA  - eng
SI  - GENBANK/KF135227
SI  - GENBANK/KF135228
SI  - GENBANK/KF135229
SI  - GENBANK/KF135230
SI  - GENBANK/KF135231
SI  - GENBANK/KF135232
SI  - GENBANK/KF135233
SI  - GENBANK/KF135234
SI  - GENBANK/KF135235
SI  - GENBANK/KF135236
SI  - GENBANK/KF135237
SI  - GENBANK/KF135238
SI  - GENBANK/KF135239
SI  - GENBANK/KF135240
SI  - GENBANK/KF135241
SI  - GENBANK/KF135242
SI  - GENBANK/KF135243
SI  - GENBANK/KF135244
SI  - GENBANK/KF135245
SI  - GENBANK/KF135246
SI  - GENBANK/KF135247
SI  - GENBANK/KF135248
SI  - GENBANK/KF135249
SI  - GENBANK/KF135250
SI  - GENBANK/KF135251
SI  - GENBANK/KF135252
SI  - GENBANK/KF135253
SI  - GENBANK/KF135254
SI  - GENBANK/KF135255
SI  - GENBANK/KF135256
SI  - GENBANK/KF135257
SI  - GENBANK/KF135258
SI  - GENBANK/KF135259
SI  - GENBANK/KF135260
SI  - GENBANK/KF135261
SI  - GENBANK/KF135262
SI  - GENBANK/KF135263
SI  - GENBANK/KF135264
SI  - GENBANK/KF135265
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mol Ecol
JT  - Molecular ecology
JID - 9214478
RN  - 0 (DNA, Bacterial)
RN  - 0 (Hydrocarbons)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Water Pollutants, Chemical)
RN  - EC 1.13.11.- (Dioxygenases)
SB  - IM
MH  - Bacteria/*genetics/isolation & purification/metabolism
MH  - Biodegradation, Environmental
MH  - DNA, Bacterial/genetics
MH  - Dioxygenases/genetics
MH  - Endophytes/*genetics
MH  - Geologic Sediments/chemistry/microbiology
MH  - Hydrocarbons/*analysis
MH  - Microbiota
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Portugal
MH  - RNA, Ribosomal, 16S/genetics
MH  - Salt-Tolerant Plants/*microbiology
MH  - Water Pollutants, Chemical/*analysis
MH  - *Wetlands
EDAT- 2014/04/26 06:00
MHDA- 2014/06/24 06:00
CRDT- 2014/04/26 06:00
PHST- 2014/04/26 06:00 [entrez]
PHST- 2014/04/26 06:00 [pubmed]
PHST- 2014/06/24 06:00 [medline]
AID - 10.1111/mec.12559 [doi]
PST - ppublish
SO  - Mol Ecol. 2014 Mar;23(6):1392-1404. doi: 10.1111/mec.12559.