PMID- 24767179
OWN - NLM
STAT- MEDLINE
DCOM- 20150204
LR  - 20211021
IS  - 1477-7819 (Electronic)
IS  - 1477-7819 (Linking)
VI  - 12
DP  - 2014 Apr 26
TI  - Clusterin silencing inhibits proliferation and reduces invasion in human 
      laryngeal squamous carcinoma cells.
PG  - 124
LID - 10.1186/1477-7819-12-124 [doi]
AB  - BACKGROUND: Clusterin is, in its major form, a secreted heterodimeric 
      disulfide-linked glycoprotein (sCLU), which plays important roles in cell 
      survival and death. In laryngeal squamous cell carcinomas (LSCC), sCLU is 
      up-regulated and its expression is related to the invasiveness of these tumors. 
      The purpose of this study was to explore the inhibiting role of sCLU gene silence 
      in the invasive ability and growth of Hep-2 human laryngeal squamous carcinoma 
      cells (Hep-2) by transfection of short hairpin RNA expression plasmids against 
      sCLU (sCLU-shRNA) (in vivo) or small interference RNA (sCLU-siRNA) (in vitro). 
      METHODS: sCLU-siRNA and the control siRNA were transfected into Hep-2 cells using 
      Lipofectamine 2000. RT-PCR and Western blot were used to detect the effect of 
      siRNA transfection on sCLU mRNA and sCLU protein expression. The invasive 
      activity of sCLU-siRNA-transfected Hep-2 cells was measured with the modified 
      Boyden chamber assay and wound healing assay. The effects of sCLU-siRNA on cell 
      proliferation were evaluated by MTT assay. Apoptosis was measured by Annexin 
      V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) double-staining 
      methods. We next evaluated the effects of sCLU silencing by sCLU-shRNA 
      transfection in vivo on tumor growth and metastatic properties to the lung. 
      Terminal deoxytransferase-mediated dUTP nick end labeling (TUNEL) staining was 
      used to observe the apoptosis in the xenografts. RESULTS: It showed that 
      siRNA-mediated down-regulation of sCLU expression in Hep-2 cells significantly 
      inhibited cell proliferation and promoted apoptosis in vitro. Furthermore, 
      siRNA-mediated down-regulation of sCLU expression decreases in vitro cell 
      migration and invasion ability. In vivo, the average volume of tumors in the 
      sCLU-shRNA transfected group was significantly lower than in the control group 
      (P<0.01), and the significant apoptosis detected with TUNEL was indicated in the 
      sCLU-shRNA transfected groups (P<0.05). Significantly, we found that sCLU-shRNA 
      could exert marked inhibition of the lung metastasis of Hep-2 cells in nude mice 
      in vivo. CONCLUSIONS: sCLU gene silence can inhibit invasion and growth of LSCC. 
      sCLU may provide a potential therapeutic target against human LSCC.
FAU - Wang, Qianjin
AU  - Wang Q
FAU - Cao, Weiyan
AU  - Cao W
FAU - Su, Quancai
AU  - Su Q
FAU - Liu, Zimin
AU  - Liu Z
FAU - Zhang, Lin
AU  - Zhang L
AD  - Department of Anesthesiology, the Affiliated Hospital of Qingdao University, 
      Qingdao 266003, China. zhanglinmzs@126.com.
LA  - eng
PT  - Journal Article
DEP - 20140426
PL  - England
TA  - World J Surg Oncol
JT  - World journal of surgical oncology
JID - 101170544
RN  - 0 (CLU protein, human)
RN  - 0 (Clusterin)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Blotting, Western
MH  - Carcinoma, Squamous Cell/metabolism/pathology/*prevention & control
MH  - Cell Adhesion
MH  - *Cell Movement
MH  - *Cell Proliferation
MH  - Clusterin/*antagonists & inhibitors/genetics/metabolism
MH  - Female
MH  - Gene Silencing
MH  - Humans
MH  - Laryngeal Neoplasms/metabolism/pathology/*prevention & control
MH  - Lung Neoplasms/metabolism/*prevention & control/secondary
MH  - Mice
MH  - Mice, SCID
MH  - Neoplasm Invasiveness
MH  - RNA, Messenger/genetics
MH  - RNA, Small Interfering/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
MH  - Wound Healing
PMC - PMC4016627
EDAT- 2014/04/29 06:00
MHDA- 2015/02/05 06:00
PMCR- 2014/04/26
CRDT- 2014/04/29 06:00
PHST- 2014/03/03 00:00 [received]
PHST- 2014/04/07 00:00 [accepted]
PHST- 2014/04/29 06:00 [entrez]
PHST- 2014/04/29 06:00 [pubmed]
PHST- 2015/02/05 06:00 [medline]
PHST- 2014/04/26 00:00 [pmc-release]
AID - 1477-7819-12-124 [pii]
AID - 10.1186/1477-7819-12-124 [doi]
PST - epublish
SO  - World J Surg Oncol. 2014 Apr 26;12:124. doi: 10.1186/1477-7819-12-124.