PMID- 24768806
OWN - NLM
STAT- MEDLINE
DCOM- 20160509
LR  - 20140616
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 154
IP  - 3
DP  - 2014 Jul 3
TI  - Investigation on the spectrum-effect relationships of Da-Huang-Fu-Zi-Tang in rats 
      by UHPLC-ESI-Q-TOF-MS method.
PG  - 606-12
LID - S0378-8741(14)00315-8 [pii]
LID - 10.1016/j.jep.2014.04.027 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: Da-Huang-Fu-Zi-Tang (DHFZT) is a crucial TCM 
      formula commonly used for the treatment of acute pancreatitis in Chinese clinical 
      application. Our previous work found that DHFZT could act against pancreatic 
      injury in rats with severe acute pancreatitis (SAP). The goal of this paper was 
      to study the underlying correlations between the chemical spectra and the 
      protective effect of DHFZT on pancreatic acinar cell to reveal the real bioactive 
      compounds in DHFZT. MATERIALS AND METHODS: The fingerprint chromatograms of rat 
      serum after oral administration of DHFZT were established by UHPLC-ESI-Q-TOF-MS 
      technique. At the same time, the model of anti-acute pancreatitis on cells was 
      established by adding 10(-7) mol/L cerulein to AR42J cell line, and the 
      protective effects of the serum on pancreatic acinar cell from injury was 
      evaluated by detecting the efficacy of amylase. Then, the spectrum-effect 
      relationships between UHPLC fingerprints and anti-acute pancreatitis activities 
      were evaluated using canonical correlation analysis (CCA) statistical method. The 
      chromatogram separation was performed on a C18 reversed phase UHPLC column (2.1 
      mm x 100 mm, 3.5 mum, Agilent), the column temperature was set at 35 degrees C. The mobile 
      phase consisted of 0.1% formic acid and acetonitrile with gradient elution. The 
      serum samples were analyzed both in negative and positive ion mode. The mother 
      and productive ions were scanned within the mass range of m/z 100-1200 and 
      50-1200, respectively. A thorough analysis of a great deal of information of the 
      constituents in the rat serum was undertaken. The structure identification of the 
      detected compounds was achieved by using high resolution MS values as well as the 
      MS/MS fragments. RESULTS: Eighteen peaks in rat serum after oral administration 
      of DHFZT were detected within only 30 min recorded chromatograms. The structure 
      of the 18 compounds were then given out, of which 10 were the original form of 
      compounds absorbed from DHFZT, 8 were the metabolites of the compounds existed in 
      rat serum. According to the CCA results, talatisamine, rhein glucoside, rhein 
      isomer methylation, hypaconine, hydroxyl-chrysophanol, emodin glucuronide 
      conjugation, and chrysophanol glucuronide conjugation were finally found to be 
      the main anti-acute pancreatitis components in DHFZT. CONCLUSIONS: The model 
      presented in this paper successfully discovered the spectrum-effect relationships 
      of DHFZT, which showed a representative way to discover the primary active 
      ingredients from the complicated herbal drugs.
CI  - Copyright (c) 2014. Published by Elsevier Ireland Ltd.
FAU - Liu, Xiao
AU  - Liu X
AD  - College of Pharmacy, Nanjing University of Chinese Medicine, 138 Xianlin Avenue, 
      Nanjing 210023, PR China; Engineering Center of State Ministry of Education for 
      Standardization of Chinese Medicine Processing, Nanjing University of Chinese 
      Medicine, Nanjing 210023, PR China; Nanjing Haichang Chinese Medicine Group 
      Corporation, Nanjing 210061, PR China; College of Science, Cleveland State 
      University, 2121 Euclid Avenue, Cleveland, OH 44115, USA.
FAU - Wang, Xiao-li
AU  - Wang XL
AD  - College of Pharmacy, Nanjing University of Chinese Medicine, 138 Xianlin Avenue, 
      Nanjing 210023, PR China; Engineering Center of State Ministry of Education for 
      Standardization of Chinese Medicine Processing, Nanjing University of Chinese 
      Medicine, Nanjing 210023, PR China; Nanjing Haichang Chinese Medicine Group 
      Corporation, Nanjing 210061, PR China.
FAU - Wu, Li
AU  - Wu L
AD  - College of Pharmacy, Nanjing University of Chinese Medicine, 138 Xianlin Avenue, 
      Nanjing 210023, PR China; Engineering Center of State Ministry of Education for 
      Standardization of Chinese Medicine Processing, Nanjing University of Chinese 
      Medicine, Nanjing 210023, PR China.
FAU - Li, Huan
AU  - Li H
AD  - College of Pharmacy, Nanjing University of Chinese Medicine, 138 Xianlin Avenue, 
      Nanjing 210023, PR China; Engineering Center of State Ministry of Education for 
      Standardization of Chinese Medicine Processing, Nanjing University of Chinese 
      Medicine, Nanjing 210023, PR China; Nanjing Haichang Chinese Medicine Group 
      Corporation, Nanjing 210061, PR China.
FAU - Qin, Kun-ming
AU  - Qin KM
AD  - College of Pharmacy, Nanjing University of Chinese Medicine, 138 Xianlin Avenue, 
      Nanjing 210023, PR China; Engineering Center of State Ministry of Education for 
      Standardization of Chinese Medicine Processing, Nanjing University of Chinese 
      Medicine, Nanjing 210023, PR China; Nanjing Haichang Chinese Medicine Group 
      Corporation, Nanjing 210061, PR China.
FAU - Cai, Hao
AU  - Cai H
AD  - College of Pharmacy, Nanjing University of Chinese Medicine, 138 Xianlin Avenue, 
      Nanjing 210023, PR China; Engineering Center of State Ministry of Education for 
      Standardization of Chinese Medicine Processing, Nanjing University of Chinese 
      Medicine, Nanjing 210023, PR China.
FAU - Pei, Ke
AU  - Pei K
AD  - College of Pharmacy, Nanjing University of Chinese Medicine, 138 Xianlin Avenue, 
      Nanjing 210023, PR China; Engineering Center of State Ministry of Education for 
      Standardization of Chinese Medicine Processing, Nanjing University of Chinese 
      Medicine, Nanjing 210023, PR China.
FAU - Liu, Ting
AU  - Liu T
AD  - Shanghai AB Sciex Analytical Instrument Trading Co., Ltd, Shanghai 200233, PR 
      China.
FAU - Cai, Bao-chang
AU  - Cai BC
AD  - College of Pharmacy, Nanjing University of Chinese Medicine, 138 Xianlin Avenue, 
      Nanjing 210023, PR China; Engineering Center of State Ministry of Education for 
      Standardization of Chinese Medicine Processing, Nanjing University of Chinese 
      Medicine, Nanjing 210023, PR China; Nanjing Haichang Chinese Medicine Group 
      Corporation, Nanjing 210061, PR China. Electronic address: bccai@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140424
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Da-Huang-Fu-Zi-Tang)
RN  - 0 (Drugs, Chinese Herbal)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Cell Line
MH  - Chromatography, High Pressure Liquid
MH  - Drugs, Chinese Herbal/administration & 
      dosage/*chemistry/pharmacology/*therapeutic use
MH  - Male
MH  - Pancreatitis/*drug therapy/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spectrometry, Mass, Electrospray Ionization
OTO - NOTNLM
OT  - Aloe-emodin (PubChem CID: 10207)
OT  - Benzoylaconine (PubChem CID: 121312)
OT  - Benzoylmesaconine (PubChem CID: 24832659)
OT  - Canonical correlation analysis
OT  - Da-Huang-Fu-Zi-Tang
OT  - Emodin (PubChem CID: 3220)
OT  - Hydroxy-chrysophanol (PubChem CID: 442731)
OT  - Hypaconine (PubChem CID: 23337)
OT  - Mesaconine (PubChem CID: 24832657)
OT  - Rhein (PubChem CID: 10168)
OT  - Severe acute pancreatitis
OT  - Spectrum-effect relationship
OT  - Talatisamine (PubChem CID: 159891)
OT  - Torachrysone (PubChem CID: 5321977)
OT  - UHPLC-ESI-Q-TOF-MS
EDAT- 2014/04/29 06:00
MHDA- 2016/05/10 06:00
CRDT- 2014/04/29 06:00
PHST- 2014/01/17 00:00 [received]
PHST- 2014/04/14 00:00 [revised]
PHST- 2014/04/15 00:00 [accepted]
PHST- 2014/04/29 06:00 [entrez]
PHST- 2014/04/29 06:00 [pubmed]
PHST- 2016/05/10 06:00 [medline]
AID - S0378-8741(14)00315-8 [pii]
AID - 10.1016/j.jep.2014.04.027 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2014 Jul 3;154(3):606-12. doi: 10.1016/j.jep.2014.04.027. Epub 
      2014 Apr 24.