PMID- 24769167
OWN - NLM
STAT- MEDLINE
DCOM- 20141215
LR  - 20161125
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1566
DP  - 2014 May 30
TI  - Chemical stimulation of the intracranial dura activates NALP3 inflammasome in 
      trigeminal ganglia neurons.
PG  - 1-11
LID - S0006-8993(14)00517-4 [pii]
LID - 10.1016/j.brainres.2014.04.019 [doi]
AB  - Inflammasomes are molecular platforms that upon activation by cellular infection 
      or stress trigger the maturation of proinflammatory cytokines such as interleukin 
      (IL)-1beta to engage innate immune defenses. Increased production of IL-1beta in pain 
      and inflammation such as headache is well documented. However, limited evidence 
      addresses the participation of inflammasomes in inflammatory pain. The present 
      study used rat inflammatory dural stimulation-induced model of intracranial pain 
      to assess whether headache-related pain can induce the activation of NACHT, LRR, 
      and PYD-containing protein (NALP)-3 inflammasome pathway in the trigeminal 
      ganglia (TG) and which cells express NALP3 inflammasome proteins and IL-1beta. 
      Chemical stimulation of the intracranial dura caused a total drug dose- and 
      time-dependent induction of activated caspase-1 and mature IL-1beta proteins. 
      Application of a selective caspase-1 inhibitor diminished these effects. 
      Immunohistochemistry revealed that both NALP3 inflammasome and IL-1beta 
      immunoreactivity were existed mainly in small to medium-sized C-type neurons and 
      increased over time, with intense cytoplasmic staining after 3 days of dural 
      inflammation. Overall, the present observation indicated that dural inflammation 
      promoted assembly of the multiprotein NALP3 complex, activated caspase-1, and 
      induced processing of IL-1beta, which provides an indirect evidence of the 
      participation of NALP3 inflammasome in the cascade of events involved in the 
      genesis of headaches by promoting IL-1beta maturation in the TG. This may contribute 
      to strategies for headache control.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Chen, Lingzhi
AU  - Chen L
AD  - Department of Neurology, the First Affiliated Hospital of Nanjing Medical 
      University, No. 300, Guangzhou Street, Nanjing, Jiangsu Province 210029, PR 
      China.
FAU - Li, Xiaohui
AU  - Li X
AD  - Department of Neurology, the First Affiliated Hospital of Nanjing Medical 
      University, No. 300, Guangzhou Street, Nanjing, Jiangsu Province 210029, PR 
      China.
FAU - Huang, Lin
AU  - Huang L
AD  - Department of Neurology, the First Affiliated Hospital of Nanjing Medical 
      University, No. 300, Guangzhou Street, Nanjing, Jiangsu Province 210029, PR 
      China.
FAU - Wu, Qian
AU  - Wu Q
AD  - Department of Neurology, the First Affiliated Hospital of Nanjing Medical 
      University, No. 300, Guangzhou Street, Nanjing, Jiangsu Province 210029, PR 
      China.
FAU - Chen, Ling
AU  - Chen L
AD  - Department of Physiology, Nanjing Medical University, 140 Hanzhong Road, Nanjing, 
      Jiangsu, 210029, PR China.
FAU - Wan, Qi
AU  - Wan Q
AD  - Department of Neurology, the First Affiliated Hospital of Nanjing Medical 
      University, No. 300, Guangzhou Street, Nanjing, Jiangsu Province 210029, PR 
      China. Electronic address: qi_wan@126.com.
LA  - eng
PT  - Journal Article
DEP - 20140423
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Carrier Proteins)
RN  - 0 (Inflammasomes)
RN  - 0 (Interleukin-1beta)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (Nlrp3 protein, rat)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - EC 3.4.22.36 (Caspase 1)
SB  - IM
MH  - Animals
MH  - Carrier Proteins
MH  - Caspase 1/metabolism
MH  - Disease Models, Animal
MH  - Dura Mater/drug effects/*metabolism
MH  - Headache/*metabolism
MH  - Inflammasomes/*metabolism
MH  - Inflammation/metabolism
MH  - Interleukin-1beta/metabolism
MH  - Male
MH  - NLR Family, Pyrin Domain-Containing 3 Protein
MH  - Neurons/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Cytoplasmic and Nuclear/*metabolism
MH  - Stimulation, Chemical
MH  - Trigeminal Ganglion/*metabolism
OTO - NOTNLM
OT  - Caspase-1
OT  - Dural inflammation
OT  - Headache
OT  - Interleukin-1beta
OT  - NALP3 inflammasome
OT  - Trigeminal Ganglion
EDAT- 2014/04/29 06:00
MHDA- 2014/12/17 06:00
CRDT- 2014/04/29 06:00
PHST- 2014/01/09 00:00 [received]
PHST- 2014/04/11 00:00 [revised]
PHST- 2014/04/12 00:00 [accepted]
PHST- 2014/04/29 06:00 [entrez]
PHST- 2014/04/29 06:00 [pubmed]
PHST- 2014/12/17 06:00 [medline]
AID - S0006-8993(14)00517-4 [pii]
AID - 10.1016/j.brainres.2014.04.019 [doi]
PST - ppublish
SO  - Brain Res. 2014 May 30;1566:1-11. doi: 10.1016/j.brainres.2014.04.019. Epub 2014 
      Apr 23.