PMID- 24769533
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 165
IP  - 2
DP  - 2014 Jun
TI  - Photobody Localization of Phytochrome B Is Tightly Correlated with Prolonged and 
      Light-Dependent Inhibition of Hypocotyl Elongation in the Dark.
PG  - 595-607
AB  - Photobody localization of Arabidopsis (Arabidopsis thaliana) phytochrome B (phyB) 
      fused to green fluorescent protein (PBG) correlates closely with the 
      photoinhibition of hypocotyl elongation. However, the amino-terminal half of phyB 
      fused to green fluorescent protein (NGB) is hypersensitive to light despite its 
      inability to localize to photobodies. Therefore, the significance of photobodies 
      in regulating hypocotyl growth remains debatable. Accumulating evidence indicates 
      that under diurnal conditions, photoactivated phyB persists into darkness to 
      inhibit hypocotyl elongation. Here, we examine whether photobodies are involved 
      in inhibiting hypocotyl growth in darkness by comparing the PBG and NGB lines 
      after the red light-to-dark transition. Surprisingly, after the transition from 
      10 mumol m(-2) s(-1) red light to darkness, PBG inhibits hypocotyl elongation 
      three times longer than NGB. The disassembly of photobodies in PBG hypocotyl 
      nuclei correlates tightly with the accumulation of the growth-promoting 
      transcription factor PHYTOCHROME-INTERACTING FACTOR3 (PIF3). Destabilizing 
      photobodies by either decreasing the light intensity or adding monochromatic 
      far-red light treatment before the light-to-dark transition leads to faster PIF3 
      accumulation and a dramatic reduction in the capacity for hypocotyl growth 
      inhibition in PBG. In contrast, NGB is defective in PIF3 degradation, and its 
      hypocotyl growth in the dark is nearly unresponsive to changes in light 
      conditions. Together, our results support the model that photobodies are required 
      for the prolonged, light-dependent inhibition of hypocotyl elongation in the dark 
      by repressing PIF3 accumulation and by stabilizing the far-red light-absorbing 
      form of phyB. Our study suggests that photobody localization patterns of phyB 
      could serve as instructive cues that control light-dependent photomorphogenetic 
      responses in the dark.
CI  - (c) 2014 American Society of Plant Biologists. All Rights Reserved.
FAU - Van Buskirk, Elise K
AU  - Van Buskirk EK
AD  - Department of Biology, Duke University, Durham, North Carolina 27708 (E.K.V.B., 
      A.K.R., M.C.); andDepartment of Botany, Graduate School of Science, Kyoto 
      University, Sakyo-ku, Kyoto 606-8502, Japan (A.N.).
FAU - Reddy, Amit K
AU  - Reddy AK
AD  - Department of Biology, Duke University, Durham, North Carolina 27708 (E.K.V.B., 
      A.K.R., M.C.); andDepartment of Botany, Graduate School of Science, Kyoto 
      University, Sakyo-ku, Kyoto 606-8502, Japan (A.N.).
FAU - Nagatani, Akira
AU  - Nagatani A
AD  - Department of Biology, Duke University, Durham, North Carolina 27708 (E.K.V.B., 
      A.K.R., M.C.); andDepartment of Botany, Graduate School of Science, Kyoto 
      University, Sakyo-ku, Kyoto 606-8502, Japan (A.N.).
FAU - Chen, Meng
AU  - Chen M
AD  - Department of Biology, Duke University, Durham, North Carolina 27708 (E.K.V.B., 
      A.K.R., M.C.); andDepartment of Botany, Graduate School of Science, Kyoto 
      University, Sakyo-ku, Kyoto 606-8502, Japan (A.N.) meng.chen@duke.edu.
LA  - eng
GR  - R01 GM087388/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20140425
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
PMC - PMC4044834
EDAT- 2014/04/29 06:00
MHDA- 2014/04/29 06:01
PMCR- 2014/04/25
CRDT- 2014/04/29 06:00
PHST- 2014/04/29 06:00 [entrez]
PHST- 2014/04/29 06:00 [pubmed]
PHST- 2014/04/29 06:01 [medline]
PHST- 2014/04/25 00:00 [pmc-release]
AID - pp.114.236661 [pii]
AID - 236661 [pii]
AID - 10.1104/pp.114.236661 [doi]
PST - ppublish
SO  - Plant Physiol. 2014 Jun;165(2):595-607. doi: 10.1104/pp.114.236661. Epub 2014 Apr 
      25.