PMID- 2477002
OWN - NLM
STAT- MEDLINE
DCOM- 19891106
LR  - 20201214
IS  - 0896-8411 (Print)
IS  - 0896-8411 (Linking)
VI  - 2
IP  - 4
DP  - 1989 Aug
TI  - A major autoepitope is present on the amino terminus of a human SS-A/Ro 
      polypeptide.
PG  - 367-74
AB  - A human SS-A/Ro antigen is present on the polypeptide component of a particle 
      composed of hyRNA and a 60 kD protein. We have now purified the Wil-2 cell 60 
      kilo dalton (kD) SS-A/Ro protein and determined its amino-terminal amino acid 
      sequence. A synthetic peptide corresponding to residues 7 to 24 of this sequence 
      (RoSP7-24) exhibited enzyme-linked immunosorbent assay (ELISA) binding activity 
      with immunodiffusion-defined, monospecific anti-SS-A/Ro sera. In addition, ELISA 
      binding of monospecific anti-SS-A/Ro sera to native SS-A/Ro antigen was partially 
      inhibited (35%) by KLH-RoSP7-24. Sera from patients known to frequently produce 
      precipitating anti-SS-A/Ro antibody (subacute cutaneous lupus erythematosus 
      [SCLE], 56 patients; Sjogren's syndrome [SS], 41 patients; mothers of infants 
      with neonatal LE [NLE], 10 individuals; infants with congenital heart block 
      [CHB], 5 patients) were tested for reactivity to RoSP7-24 in ELISA. Overall, 38% 
      of SCLE sera, 36% of SS sera, 50% of maternal NLE sera and 20% of CHB infant sera 
      had anti-RoSP7-24 binding levels greater than 2 standard deviations above the 
      mean of that of normal individuals. Of the sera which had anti-SS-A/Ro detected 
      by double immunodiffusion and/or counterimmunoelectrophoresis, 68% of SCLE 
      patients, 71% of SS patients, 55% of NLE mothers and 20% of CHB infants had 
      significantly elevated RoSP7-24 ELISA binding levels. These findings strongly 
      suggest that a major autoepitope of native human SS-A/Ro resides on the amino 
      terminal portion of the Wil-2 SS-A/Ro 60 kD polypeptide.(ABSTRACT TRUNCATED AT 
      250 WORDS)
FAU - Lieu, T S
AU  - Lieu TS
AD  - Department of Dermatology, University of Texas Health Science Center, Dallas 
      75235.
FAU - Newkirk, M M
AU  - Newkirk MM
FAU - Arnett, F C
AU  - Arnett FC
FAU - Lee, L A
AU  - Lee LA
FAU - Deng, J S
AU  - Deng JS
FAU - Capra, J D
AU  - Capra JD
FAU - Sontheimer, R D
AU  - Sontheimer RD
LA  - eng
GR  - A1-12127/PHS HHS/United States
GR  - AM-19101/AM/NIADDK NIH HHS/United States
GR  - AM07341/AM/NIADDK NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Autoimmun
JT  - Journal of autoimmunity
JID - 8812164
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (Autoantigens)
RN  - 0 (Epitopes)
RN  - 0 (Peptide Fragments)
RN  - 0 (Peptides)
RN  - 0 (RNA, Small Cytoplasmic)
RN  - 0 (RO60 protein, human)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (SS-A antibodies)
RN  - 0 (SS-A antigen)
SB  - IM
MH  - Antibodies, Antinuclear/immunology
MH  - Autoantigens/*immunology
MH  - Autoimmune Diseases/immunology
MH  - Binding Sites
MH  - Epitopes/immunology
MH  - Heart Block/congenital/immunology
MH  - Humans
MH  - Lupus Erythematosus, Cutaneous/immunology
MH  - Lupus Erythematosus, Systemic/congenital/immunology
MH  - Male
MH  - Peptide Fragments/chemical synthesis/immunology
MH  - Peptides/chemical synthesis/immunology
MH  - *RNA, Small Cytoplasmic
MH  - *Ribonucleoproteins
MH  - Sjogren's Syndrome/immunology
EDAT- 1989/08/01 00:00
MHDA- 1989/08/01 00:01
CRDT- 1989/08/01 00:00
PHST- 1989/08/01 00:00 [pubmed]
PHST- 1989/08/01 00:01 [medline]
PHST- 1989/08/01 00:00 [entrez]
AID - 10.1016/0896-8411(89)90165-0 [doi]
PST - ppublish
SO  - J Autoimmun. 1989 Aug;2(4):367-74. doi: 10.1016/0896-8411(89)90165-0.