PMID- 24770897
OWN - NLM
STAT- MEDLINE
DCOM- 20150901
LR  - 20220409
IS  - 1432-0878 (Electronic)
IS  - 0302-766X (Linking)
VI  - 357
IP  - 3
DP  - 2014 Sep
TI  - Expression of chemokine CXCL10 in dendritic-cell-like S100beta-positive cells in rat 
      anterior pituitary gland.
PG  - 757-65
LID - 10.1007/s00441-014-1864-2 [doi]
AB  - Chemokines are mostly small secreted polypeptides whose signals are mediated by 
      seven trans-membrane G-protein-coupled receptors. Their functions include the 
      control of leukocytes and the intercellular mediation of cell migration, 
      proliferation, and adhesion in several tissues. We have previously revealed that 
      the CXC chemokine ligand 12 (CXCL12) and its receptor 4 (CXCR4) are expressed in 
      the anterior pituitary gland, and that the CXCL12/CXCR4 axis evokes the migration 
      and interconnection of S100beta-protein-positive cells (S100beta-positive cells), which 
      do not produce classical anterior pituitary hormones. However, little is known of 
      the cells producing the other CXCLs and CXCRs or of their characteristics in the 
      anterior pituitary. We therefore examined whether CXCLs and CXCRs occurred in the 
      rat anterior pituitary lobe. We used reverse transcription plus the polymerase 
      chain reaction to analyze the expression of Cxcl and Cxcr and identified the 
      cells that expressed Cxcl by in situ hybridization. Transcripts of Cxcl10 and its 
      receptor (Cxcr3 and toll-like receptor 4, Tlr4) were clearly detected: cells 
      expressing Cxcl10 and Tlr4 were identified amongst S100beta-positive cells and those 
      expressing Cxcr3 amongst adrenocorticotropic hormone (ACTH)-producing cells. We 
      also investigated Cxcl10 expression in subpopulations of S100beta-positive cells. We 
      separated cultured S100beta-positive cells into the round-type (dendritic-cell-like) 
      and process-type (astrocyte- or epithelial-cell-like) by their adherent activity 
      to laminin, a component of the extracellular matrix; CXCL10 was expressed only in 
      round-type S100beta-positive cells. Thus, CXCL10 produced by a subpopulation of 
      S100beta-positive cells probably exerts an autocrine/paracrine effect on 
      S100beta-positive cells and ACTH-producing cells in the anterior lobe.
FAU - Horiguchi, Kotaro
AU  - Horiguchi K
AD  - Laboratory of Anatomy and Cell Biology, Department of Health Sciences, Kyorin 
      University, Tokyo, Japan.
FAU - Fujiwara, Ken
AU  - Fujiwara K
FAU - Higuchi, Masashi
AU  - Higuchi M
FAU - Yoshida, Saishu
AU  - Yoshida S
FAU - Tsukada, Takehiro
AU  - Tsukada T
FAU - Ueharu, Hiroki
AU  - Ueharu H
FAU - Chen, Mo
AU  - Chen M
FAU - Hasegawa, Rumi
AU  - Hasegawa R
FAU - Takigami, Shu
AU  - Takigami S
FAU - Ohsako, Shunji
AU  - Ohsako S
FAU - Yashiro, Takashi
AU  - Yashiro T
FAU - Kato, Takako
AU  - Kato T
FAU - Kato, Yukio
AU  - Kato Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140427
PL  - Germany
TA  - Cell Tissue Res
JT  - Cell and tissue research
JID - 0417625
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Ligands)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (S100 Calcium Binding Protein beta Subunit)
RN  - 0 (S100b protein, rat)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Chemokine CXCL10/*metabolism
MH  - Chemokine CXCL12/metabolism
MH  - Dendritic Cells/*metabolism
MH  - Ligands
MH  - Male
MH  - Pituitary Gland, Anterior/*cytology/*metabolism
MH  - Protein Transport
MH  - Rats, Transgenic
MH  - Rats, Wistar
MH  - Receptors, Chemokine/metabolism
MH  - S100 Calcium Binding Protein beta Subunit/*metabolism
EDAT- 2014/04/29 06:00
MHDA- 2015/09/02 06:00
CRDT- 2014/04/29 06:00
PHST- 2014/01/20 00:00 [received]
PHST- 2014/02/26 00:00 [accepted]
PHST- 2014/04/29 06:00 [entrez]
PHST- 2014/04/29 06:00 [pubmed]
PHST- 2015/09/02 06:00 [medline]
AID - 10.1007/s00441-014-1864-2 [doi]
PST - ppublish
SO  - Cell Tissue Res. 2014 Sep;357(3):757-65. doi: 10.1007/s00441-014-1864-2. Epub 
      2014 Apr 27.