PMID- 24771111
OWN - NLM
STAT- MEDLINE
DCOM- 20141209
LR  - 20160303
IS  - 1097-0215 (Electronic)
IS  - 0020-7136 (Linking)
VI  - 135
IP  - 12
DP  - 2014 Dec 15
TI  - Fluorescence in situ hybridization and qPCR to detect Merkel cell polyomavirus 
      physical status and load in Merkel cell carcinomas.
PG  - 2804-15
LID - 10.1002/ijc.28931 [doi]
AB  - The Merkel cell polyomavirus (MCPyV) is detected in 80% of Merkel cell carcinomas 
      (MCC). Clonal integration and tumor-specific mutations in the large T antigen are 
      strong arguments that MCPyV is a human tumor virus. However, the relationship 
      between viral presence and cancer induction remains discussed controversially. 
      Since almost all studies on virus prevalence are based on PCR techniques, we 
      performed MCPyV fluorescence in situ hybridization (FISH) on MCC to gain 
      information about the quality of the viral presence on the single cell level. 
      MCPyV-FISH was performed on tissue microarrays containing 62 formalin-fixed and 
      paraffin-embedded tissue samples including all tumor grades of 42 patients. The 
      hybridization patterns were correlated to the qPCR data determined on 
      corresponding whole tissue sections. Indeed, MCPyV-FISH and qPCR data were highly 
      correlated, i.e. 83% for FISH-positive and 93% for FISH-negative cores. 
      Accordingly, the mean of the qPCR values of all MCPyV-positive cores differed 
      significantly from the mean of the negative cores (p = 0.0076). Importantly, two 
      hybridization patterns were definable in the MCPyV-FISH: a punctate pattern (85%) 
      indicating viral integration, which correlated with a moderate viral abundance 
      and a combination of the punctate with a diffuse pattern (15%), suggesting a 
      possible coexistence of integrated and episomal virus which was associated with 
      very high viral load and VP1 expression. Thus, MCPyV-FISH adds important 
      information on the single cell level within the histomorphological context and 
      could therefore be an important tool to further elucidate MCPyV related 
      carcinogenesis.
CI  - (c) 2014 UICC.
FAU - Haugg, Anke M
AU  - Haugg AM
AD  - Department of Pathology, GROW-School for Oncology and Developmental Biology, 
      Maastricht UMC, Maastricht, The Netherlands.
FAU - Rennspiess, Dorit
AU  - Rennspiess D
FAU - zur Hausen, Axel
AU  - zur Hausen A
FAU - Speel, Ernst-Jan M
AU  - Speel EJ
FAU - Cathomas, Gieri
AU  - Cathomas G
FAU - Becker, Jurgen C
AU  - Becker JC
FAU - Schrama, David
AU  - Schrama D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140509
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Antigens, Viral, Tumor)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antigens, Viral, Tumor/genetics
MH  - Carcinogenesis
MH  - Carcinoma, Merkel Cell/complications/*virology
MH  - DNA, Viral/genetics
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Merkel cell polyomavirus/genetics/*physiology
MH  - Middle Aged
MH  - Mutation
MH  - Polymerase Chain Reaction
MH  - Polyomavirus Infections/complications/*virology
MH  - Skin Neoplasms/complications/*virology
MH  - Tissue Array Analysis
MH  - *Viral Load
OTO - NOTNLM
OT  - Merkel cell carcinoma
OT  - Merkel cell polyomavirus
OT  - carcinogenesis
OT  - fluorescence in situ hybridization
OT  - viral integration
OT  - viral load
EDAT- 2014/04/29 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/04/29 06:00
PHST- 2013/06/03 00:00 [received]
PHST- 2014/04/09 00:00 [accepted]
PHST- 2014/04/29 06:00 [entrez]
PHST- 2014/04/29 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - 10.1002/ijc.28931 [doi]
PST - ppublish
SO  - Int J Cancer. 2014 Dec 15;135(12):2804-15. doi: 10.1002/ijc.28931. Epub 2014 May 
      9.