PMID- 24772192
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1793-9844 (Print)
IS  - 1793-9852 (Electronic)
IS  - 1793-9844 (Linking)
VI  - 1
IP  - 3-4
DP  - 2010 Sep 1
TI  - ANALYSIS OF DENDRITIC CELL STIMULATION UTILIZING A MULTI-FACETED NANOPOLYMER 
      DELIVERY SYSTEM AND THE IMMUNE MODULATOR 1-METHYL TRYPTOPHAN.
PG  - 239-250
AB  - Dendritic cells (DCs) play a pivotal role in immune modulation. Therefore, 
      understanding and regulating the mechanism of DC activation is paramount for 
      functional optimization of any immunotherapy strategy. In particular, the 
      paradoxical ability of DCs to secrete the immune suppressive enzyme indoleamine 
      2, 3-dioxygenase (IDO) and the suppressive cytokine IL-10 during the course of, 
      and in response to, stimulation is of great interest. 1-Methyl-Tryptophan (1 MT) 
      is a known inhibitor of IDO and has thus been administered in numerous in vitro 
      and in vivo systems to block IDO activity. However, the effect 1 MT has on DCs 
      beyond inhibiting IDO, especially in therapeutic models, has rarely been 
      analyzed. In the current study, we have administered 1 MT via a nanopolymer-based 
      delivery system in conjunction with an antigen (ovalbumin, OVA) and an adjuvant 
      (CpG motif DNA) to determine both the effects of 1 MT on DCs and the resulting 
      efficacy of the polymer-based treatments. 1 MT delivery alone, either via the 
      polymer-based delivery vehicle or dissolved in solution, induced no significant 
      change in DC activation as measured by surface expression of CD80, CD86, and 
      MHCII and several secreted products such as IL-12. These same factors were 
      upregulated however, when 1 MT was delivered in conjunction with OVA and CpG. 
      Although soluble delivery of these components increased the levels of expression 
      and secretion of key proteins, a differential effect of DC stimulation was seen 
      as a result of the polymer delivery system. The T cell suppressive IL-10 
      secretion was lower with the polymer-based treatments and IL-12 immune-enhancing 
      secretion was increased when 1 MT was supplemented into the polymer system. As a 
      result, including 1 MT in the polymers along with OVA and CpG was seen to have 
      additional effects on DC stimulation and was able to shift DCs to a state more 
      indicative of inducing a Th1-type response.
FAU - Nikitczuk, Kevin P
AU  - Nikitczuk KP
AD  - Department of Biomedical Engineering, Rutgers University, 599 Taylor Road 
      Piscataway, New Jersey, USA.
FAU - Lattime, Edmund C
AU  - Lattime EC
AD  - The Cancer Institute of New Jersey, Departments of Surgery and Molecular Genetics 
      Microbiology, and Immunology, University of Medicine and Dentistry of New Jersey, 
      Robert Wood Johnson Medical School, New Brunswick, New Jersey, USA.
FAU - Schloss, Rene S
AU  - Schloss RS
AD  - Department of Biomedical Engineering, Rutgers University, 599 Taylor Road 
      Piscataway, New Jersey, USA.
FAU - Yarmush, Martin L
AU  - Yarmush ML
AD  - Department of Biomedical Engineering, Rutgers University, 599 Taylor Road 
      Piscataway, New Jersey, USA.
LA  - eng
GR  - P30 CA072720/CA/NCI NIH HHS/United States
GR  - R01 CA042908/CA/NCI NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Nano Life
JT  - Nano LIFE
JID - 101571142
PMC - PMC3998209
MID - NIHMS553493
OTO - NOTNLM
OT  - 1-methyl-tryptophan
OT  - 1MT
OT  - DC
OT  - Dendritic cells
OT  - PLGA
EDAT- 2010/09/01 00:00
MHDA- 2010/09/01 00:01
PMCR- 2014/04/24
CRDT- 2014/04/29 06:00
PHST- 2014/04/29 06:00 [entrez]
PHST- 2010/09/01 00:00 [pubmed]
PHST- 2010/09/01 00:01 [medline]
PHST- 2014/04/24 00:00 [pmc-release]
AID - 10.1142/S1793984410000171 [doi]
PST - ppublish
SO  - Nano Life. 2010 Sep 1;1(3-4):239-250. doi: 10.1142/S1793984410000171.