PMID- 24779296
OWN - NLM
STAT- MEDLINE
DCOM- 20140530
LR  - 20220409
IS  - 1433-6510 (Print)
IS  - 1433-6510 (Linking)
VI  - 60
IP  - 4
DP  - 2014
TI  - Suppression of T-type Ca2+ channels inhibited human laryngeal squamous cell 
      carcinoma cell proliferation running title: roles of T-type Ca2+ channels in LSCC 
      cell proliferation.
PG  - 621-8
AB  - BACKGROUND: Despite the tight correlation between T-type Ca2+ channels and a 
      great variety of tumors, the roles of alpha1G subunit of T-type Ca2+ channels in 
      laryngeal squamous cell carcinoma (LSCC) have not yet been investigated. METHODS: 
      In the present study, we examined the expression of alpha1G subunit of T-type 
      Ca2+ channel in human LSCC tissues and cell lines. One human laryngeal squamous 
      cell carcinoma cell line, Hep-2, was also examined for T-type channels using 
      voltage-clamp recordings. Cell proliferation assays were performed in the 
      presence or absence of T-type channel blocker mibefradil and alpha1G subunit 
      sepcific siRNA. The cell cycle was determined by flow cytometry. RESULTS: Our 
      results indicated that the a1G subunit of T-type Ca2+ channel is highly expressed 
      in human laryngeal squamous cell carcinoma tissues and cell lines. alpha1G siRNA 
      significantly down-regulated the protein expression of the alpha1G subunit. Both 
      alpha1G siRNA and mibefradil inhibited Hep-2 cell proliferation and arrested cell 
      cycle progression. CONCLUSIONS: Together, these findings suggest a functional 
      role of T-type channels in certain laryngeal carcinomas, and that inhibition of 
      T-type channels reduces cell proliferation via cell cycle arrest, suggesting that 
      the alpha1G subunit of T-type Ca2+ channel may be used as a therapeutic target 
      for treating LSCC.
FAU - Yu, Wenfa
AU  - Yu W
FAU - Wang, Ping
AU  - Wang P
FAU - Ma, Huiming
AU  - Ma H
FAU - Zhang, Guozheng
AU  - Zhang G
FAU - Yulin, Zhao
AU  - Yulin Z
FAU - Lu, Baocai
AU  - Lu B
FAU - Wang, Huiming
AU  - Wang H
FAU - Dong, Mingmin
AU  - Dong M
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Clin Lab
JT  - Clinical laboratory
JID - 9705611
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Calcium Channels, T-Type)
RN  - 0 (RNA, Small Interfering)
RN  - 27B90X776A (Mibefradil)
SB  - IM
MH  - Calcium Channel Blockers
MH  - Calcium Channels, T-Type/*metabolism
MH  - Carcinoma, Squamous Cell/*metabolism
MH  - Cell Cycle
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Humans
MH  - Laryngeal Neoplasms/*metabolism
MH  - Mibefradil
MH  - RNA, Small Interfering
EDAT- 2014/05/02 06:00
MHDA- 2014/05/31 06:00
CRDT- 2014/05/01 06:00
PHST- 2014/05/01 06:00 [entrez]
PHST- 2014/05/02 06:00 [pubmed]
PHST- 2014/05/31 06:00 [medline]
AID - 10.7754/clin.lab.2013.130614 [doi]
PST - ppublish
SO  - Clin Lab. 2014;60(4):621-8. doi: 10.7754/clin.lab.2013.130614.