PMID- 24779349
OWN - NLM
STAT- MEDLINE
DCOM- 20150310
LR  - 20240321
IS  - 1948-7193 (Electronic)
IS  - 1948-7193 (Print)
IS  - 1948-7193 (Linking)
VI  - 5
IP  - 7
DP  - 2014 Jul 16
TI  - High resolution metabolite imaging in the hippocampus following neonatal exposure 
      to the environmental toxin BMAA using ToF-SIMS.
PG  - 568-75
LID - 10.1021/cn500039b [doi]
AB  - The environmental neurotoxin beta-N-methylamino-L-alanine (BMAA) is suggested to be 
      linked with neurodegenerative disease. In a rat model, neonatal exposure to BMAA 
      induced selective uptake in the hippocampus and caused cell loss, mineralization 
      and astrogliosis as well as learning and memory impairments in adulthood. 
      Moreover, neonatal exposure resulted in increased protein ubiquitination in the 
      cornus ammonis 1 (CA1) region of the adult hippocampus indicating that BMAA may 
      induce protein aggregation. Time-of-flight secondary ion mass spectrometry 
      (ToF-SIMS) based imaging is a powerful technology for spatial profiling of small 
      molecular weight compounds in biological tissues with high chemical specificity 
      and high spatial resolution. The aim of this study was to characterize 
      neurochemical changes in the hippocampus of six month-old rats treated neonatally 
      (postnatal days 9-10) with BMAA. Multivariate data analysis of whole section 
      ToF-SIMS scans was performed to delineate anatomical regions of interest based on 
      their chemical distribution pattern. Further analysis of spectral data obtained 
      from the outlined anatomical regions, including CA1 and dentate gyrus (DG) 
      revealed BMAA-induced long-term changes. Increased levels of phospholipids and 
      protein fragments in the histopathologically altered CA1 region as well as 
      phosphate depletion in the DG were observed. Moreover, high resolution SIMS 
      imaging revealed a specific localization of phosphatidylcholine lipids, protein 
      signals and potassium in the histopathologically altered CA1. These findings 
      demonstrate that ToF-SIMS based imaging is a powerful approach for probing 
      biochemical changes in situ and might serve as promising technique for 
      investigating neurotoxin-induced brain pathology.
FAU - Hanrieder, Jorg
AU  - Hanrieder J
AD  - National Center for Imaging Mass Spectrometry, University of Gothenburg and 
      Chalmers University of Technology, SE-412 96 Gothenburg, Sweden.
FAU - Gerber, Lorenz
AU  - Gerber L
FAU - Persson Sandelius, Asa
AU  - Persson Sandelius A
FAU - Brittebo, Eva B
AU  - Brittebo EB
FAU - Ewing, Andrew G
AU  - Ewing AG
FAU - Karlsson, Oskar
AU  - Karlsson O
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140509
PL  - United States
TA  - ACS Chem Neurosci
JT  - ACS chemical neuroscience
JID - 101525337
RN  - 0 (Amino Acids, Diamino)
RN  - 0 (Cyanobacteria Toxins)
RN  - 0 (Hazardous Substances)
RN  - 108SA6URTV (beta-N-methylamino-L-alanine)
SB  - IM
MH  - Amino Acids, Diamino/*toxicity
MH  - Animals
MH  - Animals, Newborn
MH  - Brain Diseases/*chemically induced/*physiopathology
MH  - Cyanobacteria Toxins
MH  - Disease Models, Animal
MH  - Gliosis/chemically induced/physiopathology
MH  - Hazardous Substances
MH  - Hippocampus/growth & development/*physiopathology
MH  - Immunohistochemistry
MH  - Male
MH  - Mass Spectrometry/*methods
MH  - Multivariate Analysis
MH  - Rats, Wistar
MH  - Signal Processing, Computer-Assisted
PMC - PMC4102959
EDAT- 2014/05/02 06:00
MHDA- 2015/03/11 06:00
PMCR- 2015/07/16
CRDT- 2014/05/01 06:00
PHST- 2014/05/01 06:00 [entrez]
PHST- 2014/05/02 06:00 [pubmed]
PHST- 2015/03/11 06:00 [medline]
PHST- 2015/07/16 00:00 [pmc-release]
AID - 10.1021/cn500039b [doi]
PST - ppublish
SO  - ACS Chem Neurosci. 2014 Jul 16;5(7):568-75. doi: 10.1021/cn500039b. Epub 2014 May 
      9.