PMID- 24780433
OWN - NLM
STAT- MEDLINE
DCOM- 20150105
LR  - 20171116
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 118
DP  - 2014 Jun 1
TI  - Temperature-responsive peptide-mimetic coating based on 
      poly(N-methacryloyl-l-leucine): properties, protein adsorption and cell growth.
PG  - 270-9
LID - S0927-7765(14)00181-7 [pii]
LID - 10.1016/j.colsurfb.2014.03.049 [doi]
AB  - Poly(N-methacryloyl-l-leucine) (PNML) coatings were successfully fabricated via 
      polymerization from peroxide initiator grafted to premodified glass substrate. 
      Chemical composition and thickness of PNML coatings were determined using time of 
      flight-secondary ion mass spectrometry (TOF- SIMS) and ellipsometry, 
      respectively. PNML coatings exhibit thermal response of the wettability, between 
      4 and 28 degrees C, which indicates a transition between hydrated loose coils and 
      hydrophobic collapsed chains. Morphology of the PNML coating was observed with 
      the AFM, transforming with increasing temperature from initially relatively 
      smooth surface to rough and more structured surface. Protein adsorption observed 
      by fluorescence microscopy for model proteins (bovine serum albumin and lentil 
      lectin labeled with fluorescein isothiocyanate) at transition from 5 to 25 degrees C, 
      showed high affinity of PNML coating to proteins at all investigated temperatures 
      and pH. Thus, PNML coating have significant potential for medical and 
      biotechnological applications as protein capture agents or functional 
      replacements of antibodies ("plastic antibodies"). The high proliferation growth 
      of the human embryonic kidney cell (HEK 293) onto PNML coating was demonstrated, 
      indicating its excellent cytocompatibility.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Raczkowska, Joanna
AU  - Raczkowska J
AD  - Smoluchowski Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 
      Krakow, Poland. Electronic address: joanna.raczkowska@uj.edu.pl.
FAU - Ohar, Mariya
AU  - Ohar M
AD  - "Lvivska Polytechnika" National University, S. Bandery 12, 79013 Lviv, Ukraine.
FAU - Stetsyshyn, Yurij
AU  - Stetsyshyn Y
AD  - "Lvivska Polytechnika" National University, S. Bandery 12, 79013 Lviv, Ukraine. 
      Electronic address: yrstecushun@ukr.net.
FAU - Zemla, Joanna
AU  - Zemla J
AD  - Smoluchowski Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 
      Krakow, Poland.
FAU - Awsiuk, Kamil
AU  - Awsiuk K
AD  - Smoluchowski Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 
      Krakow, Poland.
FAU - Rysz, Jakub
AU  - Rysz J
AD  - Smoluchowski Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 
      Krakow, Poland.
FAU - Fornal, Katarzyna
AU  - Fornal K
AD  - Smoluchowski Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 
      Krakow, Poland.
FAU - Bernasik, Andrzej
AU  - Bernasik A
AD  - AGH University of Science and Technology, Faculty of Physics and Applied Computer 
      Science, al. A. Mickiewicza 30, 30-059 Krakow, Poland.
FAU - Ohar, Halyna
AU  - Ohar H
AD  - "Lvivska Polytechnika" National University, S. Bandery 12, 79013 Lviv, Ukraine.
FAU - Fedorova, Svitlana
AU  - Fedorova S
AD  - Institute of Animal Biology, National Academy of Agrarian Sciences of Ukrainian, 
      Vasyl' Stus Str. 38, 79034 Lviv, Ukraine.
FAU - Shtapenko, Oksana
AU  - Shtapenko O
AD  - Institute of Animal Biology, National Academy of Agrarian Sciences of Ukrainian, 
      Vasyl' Stus Str. 38, 79034 Lviv, Ukraine.
FAU - Polovkovych, Svyatoslav
AU  - Polovkovych S
AD  - "Lvivska Polytechnika" National University, S. Bandery 12, 79013 Lviv, Ukraine.
FAU - Novikov, Volodymyr
AU  - Novikov V
AD  - "Lvivska Polytechnika" National University, S. Bandery 12, 79013 Lviv, Ukraine.
FAU - Budkowski, Andrzej
AU  - Budkowski A
AD  - Smoluchowski Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 
      Krakow, Poland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140406
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Peptides)
RN  - 0 (methacryloyl-L-leucine)
RN  - 059QF0KO0R (Water)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Proliferation/drug effects
MH  - Coated Materials, Biocompatible/*pharmacology
MH  - HEK293 Cells
MH  - Humans
MH  - Leucine/*analogs & derivatives/pharmacology
MH  - Microscopy, Atomic Force
MH  - Microscopy, Fluorescence
MH  - Peptides/chemistry/*pharmacology
MH  - Polymerization/drug effects
MH  - Serum Albumin, Bovine/*isolation & purification
MH  - Spectrometry, Mass, Secondary Ion
MH  - *Temperature
MH  - Water/chemistry
MH  - Wettability/drug effects
OTO - NOTNLM
OT  - Biomimetic polymer coatings
OT  - Graft polymerization
OT  - Poly(N-methacryloyl amino acids)
OT  - Protein adsorption
OT  - Temperature-responsive polymer coatings
OT  - Wettability
EDAT- 2014/05/02 06:00
MHDA- 2015/01/06 06:00
CRDT- 2014/05/01 06:00
PHST- 2013/11/10 00:00 [received]
PHST- 2014/03/27 00:00 [revised]
PHST- 2014/03/28 00:00 [accepted]
PHST- 2014/05/01 06:00 [entrez]
PHST- 2014/05/02 06:00 [pubmed]
PHST- 2015/01/06 06:00 [medline]
AID - S0927-7765(14)00181-7 [pii]
AID - 10.1016/j.colsurfb.2014.03.049 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2014 Jun 1;118:270-9. doi: 
      10.1016/j.colsurfb.2014.03.049. Epub 2014 Apr 6.