PMID- 24780928
OWN - NLM
STAT- MEDLINE
DCOM- 20150217
LR  - 20211021
IS  - 1523-1747 (Electronic)
IS  - 0022-202X (Linking)
VI  - 134
IP  - 11
DP  - 2014 Nov
TI  - Role of ROS and HMGB1 in contact allergen-induced IL-18 production in human 
      keratinocytes.
PG  - 2719-2727
LID - S0022-202X(15)36529-5 [pii]
LID - 10.1038/jid.2014.203 [doi]
AB  - Keratinocytes have a key role in all phases of allergic contact dermatitis. We 
      have recently identified the possibility to use IL-18 production for the in vitro 
      identification of contact allergens. The purpose of this study was to 
      characterize the molecular mechanisms underlying allergen-induced IL-18 
      production, in order to identify the cellular source of reactive oxygen species 
      (ROS) and the danger signals involved. The NCTC2544 cell line was exposed to 
      three contact allergens, namely p-phenylenediamine (PPD), 
      2,4-dinitrochlorobenzene (DNCB), and citral, in the presence or absence of 
      diphenylene iodonium (DPI), allopurinol, and rotenone to identify the source of 
      ROS, and to anti-Toll-like receptor 4 antibody and glycirrizic acid to 
      characterize the danger-associated molecular pattern molecules. In the case of 
      PPD, the induction of IL-18 can be modulated by rotenone, allopurinol, and DPI. 
      In the case of DNCB, rotenone completely prevents the induction of IL-18, whereas 
      for citral, DPI completely prevents the induction of IL-18. We demonstrated the 
      ability of all allergens tested to induce the release of high-mobility group 
      protein B1 (HMGB1). Its sequester by glycirrizic acid significantly modulates 
      PPD-induced IL-18 production and completely prevents DNCB- and citral-induced 
      IL-18. We found that different intracellular sources of ROS are triggered by 
      contact allergens, and an important role for HMGB1 in chemical allergen-induced 
      IL-18 production was demonstrated.
FAU - Galbiati, Valentina
AU  - Galbiati V
AD  - Laboratory of Toxicology, DiSFeB, Universita degli Studi di Milano, Milan, Italy. 
      Electronic address: valentina.galbiati@unimi.it.
FAU - Papale, Angela
AU  - Papale A
AD  - Laboratory of Toxicology, DiSFeB, Universita degli Studi di Milano, Milan, Italy.
FAU - Galli, Corrado L
AU  - Galli CL
AD  - Laboratory of Toxicology, DiSFeB, Universita degli Studi di Milano, Milan, Italy.
FAU - Marinovich, Marina
AU  - Marinovich M
AD  - Laboratory of Toxicology, DiSFeB, Universita degli Studi di Milano, Milan, Italy.
FAU - Corsini, Emanuela
AU  - Corsini E
AD  - Laboratory of Toxicology, DiSFeB, Universita degli Studi di Milano, Milan, Italy.
LA  - eng
PT  - Journal Article
DEP - 20140429
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (Acyclic Monoterpenes)
RN  - 0 (Allergens)
RN  - 0 (Cytokines)
RN  - 0 (Dinitrochlorobenzene)
RN  - 0 (HMGB1 Protein)
RN  - 0 (HMGB1 protein, human)
RN  - 0 (Interleukin-18)
RN  - 0 (Monoterpenes)
RN  - 0 (Onium Compounds)
RN  - 0 (Phenylenediamines)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 6FO62043WK (Glycyrrhizic Acid)
RN  - 6HJ411TU98 (diphenyleneiodonium)
RN  - EC 3.4.22.36 (Caspase 1)
RN  - T7EU0O9VPP (citral)
RN  - U770QIT64J (4-phenylenediamine)
SB  - IM
MH  - Acyclic Monoterpenes
MH  - Allergens/*chemistry
MH  - Caspase 1/metabolism
MH  - Cell Line
MH  - Cell Survival
MH  - Cytokines/metabolism
MH  - Dermatitis, Allergic Contact/metabolism
MH  - Dinitrochlorobenzene/chemistry
MH  - Epidermis/metabolism
MH  - Glycyrrhizic Acid/chemistry
MH  - HMGB1 Protein/*metabolism
MH  - Humans
MH  - Inflammation/metabolism
MH  - Interleukin-18/*metabolism
MH  - Keratinocytes/*metabolism
MH  - Monoterpenes/chemistry
MH  - Onium Compounds/chemistry
MH  - Phenylenediamines/chemistry
MH  - Reactive Oxygen Species/*metabolism
MH  - Toll-Like Receptor 4/metabolism
EDAT- 2014/05/02 06:00
MHDA- 2015/02/18 06:00
CRDT- 2014/05/01 06:00
PHST- 2013/09/12 00:00 [received]
PHST- 2014/03/18 00:00 [revised]
PHST- 2014/04/07 00:00 [accepted]
PHST- 2014/05/01 06:00 [entrez]
PHST- 2014/05/02 06:00 [pubmed]
PHST- 2015/02/18 06:00 [medline]
AID - S0022-202X(15)36529-5 [pii]
AID - 10.1038/jid.2014.203 [doi]
PST - ppublish
SO  - J Invest Dermatol. 2014 Nov;134(11):2719-2727. doi: 10.1038/jid.2014.203. Epub 
      2014 Apr 29.