PMID- 24782110
OWN - NLM
STAT- MEDLINE
DCOM- 20150406
LR  - 20220410
IS  - 2190-3883 (Electronic)
IS  - 1234-1983 (Linking)
VI  - 55
IP  - 3
DP  - 2014 Aug
TI  - Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, 
      pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis.
PG  - 313-8
LID - 10.1007/s13353-014-0215-z [doi]
AB  - Hybrids derived from wheat (Triticum aestivum L.) x rye (Secale cereale L.) have 
      been widely studied because of their important roles in wheat cultivar 
      improvement. Repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and 
      pAWRC.1 are usually used as probes in fluorescence in situ hybridization (FISH) 
      analysis of wheat, rye, and hybrids derived from wheat x rye. Usually, some of 
      these repetitive sequences for FISH analysis were needed to be amplified from a 
      bacterial plasmid, extracted from bacterial cells, and labeled by nick 
      translation. Therefore, the conventional procedure of probe preparation using 
      these repetitive sequences is time-consuming and labor-intensive. In this study, 
      some appropriate oligonucleotide probes have been developed which can replace the 
      roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 
      in FISH analysis of wheat, rye, and hybrids derived from wheat x rye. These 
      oligonucleotides can be synthesized easily and cheaply. Therefore, FISH analysis 
      of wheat and hybrids derived from wheat x rye using these oligonucleotide probes 
      becomes easier and more economical.
FAU - Tang, Zongxiang
AU  - Tang Z
AD  - State Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural 
      University, Wenjiang, Chengdu, 611130, Sichuan, People's Republic of China.
FAU - Yang, Zujun
AU  - Yang Z
FAU - Fu, Shulan
AU  - Fu S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140430
PL  - England
TA  - J Appl Genet
JT  - Journal of applied genetics
JID - 9514582
RN  - 0 (DNA, Plant)
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Chimera/*genetics
MH  - Chromosomes, Plant/genetics
MH  - DNA, Plant/genetics
MH  - Genetic Variation/genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Oligonucleotide Probes/*chemistry/genetics
MH  - Repetitive Sequences, Nucleic Acid/*genetics
MH  - Secale/*genetics
MH  - Triticum/*genetics
EDAT- 2014/05/02 06:00
MHDA- 2015/04/07 06:00
CRDT- 2014/05/01 06:00
PHST- 2014/02/27 00:00 [received]
PHST- 2014/04/04 00:00 [accepted]
PHST- 2014/04/04 00:00 [revised]
PHST- 2014/05/01 06:00 [entrez]
PHST- 2014/05/02 06:00 [pubmed]
PHST- 2015/04/07 06:00 [medline]
AID - 10.1007/s13353-014-0215-z [doi]
PST - ppublish
SO  - J Appl Genet. 2014 Aug;55(3):313-8. doi: 10.1007/s13353-014-0215-z. Epub 2014 Apr 
      30.