PMID- 24782172
OWN - NLM
STAT- MEDLINE
DCOM- 20141210
LR  - 20161125
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 13
IP  - 2
DP  - 2014 Apr 17
TI  - Effects of mycophenolate mofetil on the expression of monocyte chemoattractant 
      protein-1 and fibronectin in high glucose cultured human mesangial cells.
PG  - 3154-61
LID - 10.4238/2014.April.17.11 [doi]
AB  - The effects of high glucose on the expression of monocyte chemoattractant 
      protein-1 (MCP-1) and the main component of the extracellular matrix, fibronectin 
      (FN), were explored in human mesangial cells (HMCs), along with the intervention 
      effects of mycophenolate mofetil (MMF) on these indicators. Cultured HMCs were 
      divided into five groups: 1) normal control group (5 mM glucose); 2) high glucose 
      group (30 mM glucose); 3) mannitol osmotic pressure control group (5 mM glucose + 
      25 mM mannitol); 4) high glucose + MMF-10 group (30 mM glucose + 10 mug/mL MMF); 
      5) high glucose + MMF-100 group (30 mM glucose + 100 mug/mL MMF). At 24, 48, and 
      72 h, reverse transcription-polymerase chain reaction and enzyme-linked 
      immunosorbent assay methods were used to detect the effects of MMF on MCP-1 mRNA 
      and protein and FN expression in HMCs under high glucose conditions. MCP-1 mRNA 
      and protein expressions and FN secretion significantly increased in HMCs of the 
      high glucose group compared with the normal control group (P < 0.01), with the 
      highest expression observed at 48 h. MMF could reduce the MCP-1 mRNA and protein 
      and FN expression levels (P < 0.01), and the inhibition occurred in a dose- and 
      time-dependent manner (P < 0.05). In conclusion, MMF could inhibit MCP-1 
      expression and the secretion of FN, indicating that it may delay the progression 
      of glomerulosclerosis and interstitial fibrosis in diabetic nephropathy to 
      ultimately achieve protective effects on the kidney.
FAU - Chen, F Q
AU  - Chen FQ
AD  - Department of Geriatrics, The First Affiliated Hospital of China Medical 
      University, Shenyang, China.
FAU - Wang, Q Y
AU  - Wang QY
AD  - Department of Endocrinology, The First Affiliated Hospital of China Medical 
      University, Shenyang, China qiuyuewang_2013@163.com.
FAU - Wei, G Z
AU  - Wei GZ
AD  - Department of Radiology, Orthopedic Hospital of Shenyang, Shenyang, China.
FAU - Ma, X Y
AU  - Ma XY
AD  - Department of Geriatrics, The First Affiliated Hospital of China Medical 
      University, Shenyang, China.
FAU - Ma, D W
AU  - Ma DW
AD  - Department of Endocrinology, The First Affiliated Hospital of China Medical 
      University, Shenyang, China.
FAU - Deng, W W
AU  - Deng WW
AD  - Department of Geriatrics, The First Affiliated Hospital of China Medical 
      University, Shenyang, China.
FAU - Sun, W B
AU  - Sun WB
AD  - Department of Endocrinology, The First Affiliated Hospital of China Medical 
      University, Shenyang, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140417
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
RN  - 0 (Chemokine CCL2)
RN  - 0 (Culture Media)
RN  - 0 (Fibronectins)
RN  - HU9DX48N0T (Mycophenolic Acid)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis
MH  - Culture Media
MH  - Diabetic Nephropathies/drug therapy/pathology
MH  - Fibronectins/*biosynthesis
MH  - Gene Expression Regulation/*drug effects
MH  - Glucose/pharmacology
MH  - Humans
MH  - Kidney/metabolism/pathology
MH  - Mesangial Cells/*metabolism
MH  - Mycophenolic Acid/administration & dosage/analogs & derivatives
EDAT- 2014/05/02 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/05/01 06:00
PHST- 2014/05/01 06:00 [entrez]
PHST- 2014/05/02 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - gmr3322 [pii]
AID - 10.4238/2014.April.17.11 [doi]
PST - epublish
SO  - Genet Mol Res. 2014 Apr 17;13(2):3154-61. doi: 10.4238/2014.April.17.11.