PMID- 24782711
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140430
LR  - 20211021
IS  - 1662-5102 (Print)
IS  - 1662-5102 (Electronic)
IS  - 1662-5102 (Linking)
VI  - 8
DP  - 2014
TI  - High abundance of BDNF within glutamatergic presynapses of cultured hippocampal 
      neurons.
PG  - 107
LID - 10.3389/fncel.2014.00107 [doi]
LID - 107
AB  - In the mammalian brain, the neurotrophin brain-derived neurotrophic factor (BDNF) 
      has emerged as a key factor for synaptic refinement, plasticity and learning. 
      Although BDNF-induced signaling cascades are well known, the spatial aspects of 
      the synaptic BDNF localization remained unclear. Recent data provide strong 
      evidence for an exclusive presynaptic location and anterograde secretion of 
      endogenous BDNF at synapses of the hippocampal circuit. In contrast, various 
      studies using BDNF overexpression in cultured hippocampal neurons support the 
      idea that postsynaptic elements and other dendritic structures are the 
      preferential sites of BDNF localization and release. In this study we used 
      rigorously tested anti-BDNF antibodies and achieved a dense labeling of 
      endogenous BDNF close to synapses. Confocal microscopy showed natural BDNF close 
      to many, but not all glutamatergic synapses, while neither GABAergic synapses nor 
      postsynaptic structures carried a typical synaptic BDNF label. To visualize the 
      BDNF distribution within the fine structure of synapses, we implemented super 
      resolution fluorescence imaging by direct stochastic optical reconstruction 
      microscopy (dSTORM). Two-color dSTORM images of neurites were acquired with a 
      spatial resolution of ~20 nm. At this resolution, the synaptic scaffold proteins 
      Bassoon and Homer exhibit hallmarks of mature synapses and form juxtaposed bars, 
      separated by a synaptic cleft. BDNF imaging signals form granule-like clusters 
      with a mean size of ~60 nm and are preferentially found within the fine structure 
      of the glutamatergic presynapse. Individual glutamatergic presynapses carried up 
      to 90% of the synaptic BDNF immunoreactivity, and only a minor fraction of BDNF 
      molecules was found close to the postsynaptic bars. Our data proof that 
      hippocampal neurons are able to enrich and store high amounts of BDNF in small 
      granules within the mature glutamatergic presynapse, at a principle site of 
      synaptic plasticity.
FAU - Andreska, Thomas
AU  - Andreska T
AD  - Institute for Clinical Neurobiology, University Hospital, 
      Julius-Maximilians-University Wurzburg Wurzburg, Germany.
FAU - Aufmkolk, Sarah
AU  - Aufmkolk S
AD  - Department of Biotechnology and Biophysics, Biocenter, 
      Julius-Maximilians-University Wurzburg Wurzburg, Germany.
FAU - Sauer, Markus
AU  - Sauer M
AD  - Department of Biotechnology and Biophysics, Biocenter, 
      Julius-Maximilians-University Wurzburg Wurzburg, Germany.
FAU - Blum, Robert
AU  - Blum R
AD  - Institute for Clinical Neurobiology, University Hospital, 
      Julius-Maximilians-University Wurzburg Wurzburg, Germany.
LA  - eng
PT  - Journal Article
DEP - 20140411
PL  - Switzerland
TA  - Front Cell Neurosci
JT  - Frontiers in cellular neuroscience
JID - 101477935
PMC - PMC3990111
OTO - NOTNLM
OT  - BDNF
OT  - hippocampal neurons
OT  - presynapse
OT  - synapse structure
OT  - synaptic localization
EDAT- 2014/05/02 06:00
MHDA- 2014/05/02 06:01
PMCR- 2014/01/01
CRDT- 2014/05/01 06:00
PHST- 2014/01/31 00:00 [received]
PHST- 2014/03/25 00:00 [accepted]
PHST- 2014/05/01 06:00 [entrez]
PHST- 2014/05/02 06:00 [pubmed]
PHST- 2014/05/02 06:01 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - 10.3389/fncel.2014.00107 [doi]
PST - epublish
SO  - Front Cell Neurosci. 2014 Apr 11;8:107. doi: 10.3389/fncel.2014.00107. 
      eCollection 2014.