PMID- 24783569
OWN - NLM
STAT- MEDLINE
DCOM- 20150817
LR  - 20181202
IS  - 1000-0593 (Print)
IS  - 1000-0593 (Linking)
VI  - 34
IP  - 1
DP  - 2014 Jan
TI  - Sensitive determination of hemoglobin in human blood and serum by flow injection 
      coupled with chemiluminescence detection.
PG  - 241-5
AB  - A sensitive method to detect hemoglobin (Hb) by flow injection (FI) coupled with 
      chemiluminescence (CL) detection was described, which based on Hb strong 
      enhancing effect on weak luminol-hydrazine CL system in alkaline medium. 
      Parameters affecting the CL detection conditions and FI-CL system were optimized. 
      The effects of possible coexisting substances in human blood and serum of 
      detection Hb were evaluated. Under the optimum conditions, the net CL intensity 
      versus Hb concentration was linear in the range of 5.0 x 10(-9) - 6.0 x 10(-5) g 
      x mL(-1) with the detection limit of 5.8 x 10(-10) g x mL(-1) (9.0 x 10(-12) mol 
      x L(-1)). The relative standard deviations (RSDs) for 8 replicate determinations 
      of 5.0 x 10(-7) and 3.0 x 10(-6) g x mL(-1) Hb were 1.6% and 1.5%, respectively. 
      In addition, the recoveries of Hb in human blood and serum were carried out and 
      varied from 83.0% to 101.0%. The proposed method has been successfully applied 
      for the determination of Hb in healthy human blood and serum. The possible 
      mechanism of Hb enhancing the weak CL emission of luminol-hydrazine system in 
      NaOH solution was discussed by fluorescence spectrophotometer and UV-Vis 
      spectrophotometer.
FAU - Cao, Jun-Tao
AU  - Cao JT
FAU - Wang, Hui
AU  - Wang H
FAU - Chen, Yong-Hong
AU  - Chen YH
FAU - Liu, Yan-Ming
AU  - Liu YM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Guang Pu Xue Yu Guang Pu Fen Xi
JT  - Guang pu xue yu guang pu fen xi = Guang pu
JID - 9424805
RN  - 0 (Hemoglobins)
RN  - 0 (Hydrazines)
RN  - 27RFH0GB4R (hydrazine)
RN  - 5EXP385Q4F (Luminol)
SB  - IM
MH  - Blood Chemical Analysis/*methods
MH  - Flow Injection Analysis
MH  - Hemoglobins/*analysis
MH  - Humans
MH  - Hydrazines
MH  - Limit of Detection
MH  - Luminescence
MH  - *Luminescent Measurements
MH  - Luminol
MH  - Spectrometry, Fluorescence
EDAT- 2014/05/03 06:00
MHDA- 2015/08/19 06:00
CRDT- 2014/05/03 06:00
PHST- 2014/05/03 06:00 [entrez]
PHST- 2014/05/03 06:00 [pubmed]
PHST- 2015/08/19 06:00 [medline]
PST - ppublish
SO  - Guang Pu Xue Yu Guang Pu Fen Xi. 2014 Jan;34(1):241-5.