PMID- 24784759
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 165
IP  - 2
DP  - 2014 Jun
TI  - Global Regulation of Embryonic Patterning in Arabidopsis by MicroRNAs.
PG  - 670-687
AB  - The development of the embryo in Arabidopsis (Arabidopsis thaliana) involves a 
      carefully controlled set of cell divisions and cell fate decisions that lead to a 
      mature embryo containing shoot and root meristems and all basic tissue types. 
      Over the last 20 years, a number of transcriptional regulators of embryonic 
      patterning have been described, but little is known about the role of 
      posttranscriptional regulators such as microRNAs (miRNAs). Previous work has 
      centered on the study of null or very weak alleles of miRNA biosynthetic genes, 
      but these mutants either arrest early in embryogenesis or have wild-type-looking 
      embryos. Here, we significantly extend those analyses by characterizing embryos 
      mutant for a strong hypomorphic allele of DICER-LIKE1 (dcl1-15). Our data 
      demonstrate that miRNAs are required for the patterning of most regions of the 
      embryo, with the exception of the protoderm. In mutant embryos with the most 
      severe morphological defects, the majority of tissue identities are lost. 
      Different levels of miRNAs appear to be required to specify cell fates in various 
      regions of the embryo. The suspensor needs the lowest levels, followed by the 
      root apical meristem and hypocotyl, cotyledons, and shoot apical meristem. 
      Furthermore, we show that erecta acts as a suppressor of dcl1-15, a novel role 
      for this signaling pathway in embryos. Our results also indicate that the 
      regulation of the messenger RNA levels of miRNA targets involves not just the 
      action of miRNAs but has a significant transcriptional component as well.
CI  - (c) 2014 American Society of Plant Biologists. All Rights Reserved.
FAU - Seefried, William F
AU  - Seefried WF
AD  - Department of Biology, Franklin and Marshall College, Lancaster, Pennsylvania 
      17604-3003 (W.F.S., R.L.C., P.D.J.); andDepartment of Biology, University of 
      Pennsylvania, Philadelphia, Pennsylvania 19104 (M.R.W.).
FAU - Willmann, Matthew R
AU  - Willmann MR
AUID- ORCID: 0000-0002-4146-8158
AD  - Department of Biology, Franklin and Marshall College, Lancaster, Pennsylvania 
      17604-3003 (W.F.S., R.L.C., P.D.J.); andDepartment of Biology, University of 
      Pennsylvania, Philadelphia, Pennsylvania 19104 (M.R.W.).
FAU - Clausen, Rachel L
AU  - Clausen RL
AD  - Department of Biology, Franklin and Marshall College, Lancaster, Pennsylvania 
      17604-3003 (W.F.S., R.L.C., P.D.J.); andDepartment of Biology, University of 
      Pennsylvania, Philadelphia, Pennsylvania 19104 (M.R.W.).
FAU - Jenik, Pablo D
AU  - Jenik PD
AUID- ORCID: 0000-0002-8804-4942
AD  - Department of Biology, Franklin and Marshall College, Lancaster, Pennsylvania 
      17604-3003 (W.F.S., R.L.C., P.D.J.); andDepartment of Biology, University of 
      Pennsylvania, Philadelphia, Pennsylvania 19104 (M.R.W.) pjenik@fandm.edu.
LA  - eng
PT  - Journal Article
DEP - 20140501
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
PMC - PMC4044841
EDAT- 2014/05/03 06:00
MHDA- 2014/05/03 06:01
PMCR- 2014/05/01
CRDT- 2014/05/03 06:00
PHST- 2014/05/03 06:00 [entrez]
PHST- 2014/05/03 06:00 [pubmed]
PHST- 2014/05/03 06:01 [medline]
PHST- 2014/05/01 00:00 [pmc-release]
AID - pp.114.240846 [pii]
AID - 240846 [pii]
AID - 10.1104/pp.114.240846 [doi]
PST - ppublish
SO  - Plant Physiol. 2014 Jun;165(2):670-687. doi: 10.1104/pp.114.240846. Epub 2014 May 
      1.