PMID- 24787702
OWN - NLM
STAT- MEDLINE
DCOM- 20150511
LR  - 20161125
IS  - 2047-2412 (Electronic)
IS  - 2047-2404 (Linking)
VI  - 15
IP  - 9
DP  - 2014 Sep
TI  - Echocardiographic integrated backscatter for the differentiation between aortic 
      valve calcification and valvular myxoid degeneration in rats.
PG  - 1042-7
LID - 10.1093/ehjci/jeu067 [doi]
AB  - AIMS: Calcification is an independent predictor of mortality in aortic valve (AV) 
      stenosis. Echocardiographic calibrated integrated backscatter (cIB) is a 
      promising parameter for quantifying AV calcification. However, the ability of cIB 
      to differentiate between calcification and valvular thickening has been 
      questioned. Therefore, we aimed to use cIB to study AV calcification compared 
      with non-calcified AV thickening in rats, with histology as reference. METHODS 
      AND RESULTS: Twenty male Wistar rats were studied. Group 1 (N = 6) received 
      subcutaneous (SC) serotonin injections (60 mg/kg/day) for 12 weeks to induce 
      myxoid non-calcified AV thickening. Group 2 (N = 7) received vitamin D3 (25,000 
      UI/kg/day) SC to induce AV calcification, and Group 3 (N = 7) received only 
      vehicle SC for 10 weeks. cIB of the AV was calculated at the end of the study, 
      followed by measurement of the percentage of the histological AV calcification. 
      At the end of the study, cIB values and calcification percentages were 
      significantly higher in vitamin D3-injected rats compared with serotonin-injected 
      rats and controls. There was no significant difference in cIB values between 
      serotonin-injected rats and controls (vitamin D3: 21.5 +/- 3.0 dB*; serotonin: 11.8 
      +/- 3.1 dB; control: 10.3 +/- 3.4 dB; *P < 0.05). The percentage of histological 
      calcification was significantly higher in the vitamin D3 group compared with the 
      other groups. Serotonin-injected rats developed significant AV thickening. 
      CONCLUSION: Increased cIB values of the AV are related to increased calcification 
      at histology and not to myxoid non-calcified valvular thickening. Therefore, cIB 
      may be considered as a sensitive technique to quantify calcification of AV rather 
      than for detecting non-calcified valvular thickening.
CI  - Published on behalf of the European Society of Cardiology. All rights reserved. (c) 
      The Author 2014. For permissions please email: journals.permissions@oup.com.
FAU - Gillis, Kris
AU  - Gillis K
AD  - Department of Cardiology, Centrum Voor Hart-en Vaatziekten (CHVZ), UZ Brussel, 
      Laarbeeklaan 101, 1090 Jette, Belgium In Vivo Cellular and Molecular Imaging 
      Laboratory (ICMI), Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel 
      (VUB), Jette, Belgium kgillis@vub.ac.be.
FAU - Bala, Gezim
AU  - Bala G
AD  - Department of Cardiology, Centrum Voor Hart-en Vaatziekten (CHVZ), UZ Brussel, 
      Laarbeeklaan 101, 1090 Jette, Belgium In Vivo Cellular and Molecular Imaging 
      Laboratory (ICMI), Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel 
      (VUB), Jette, Belgium.
FAU - Roosens, Bram
AU  - Roosens B
AD  - Department of Cardiology, Centrum Voor Hart-en Vaatziekten (CHVZ), UZ Brussel, 
      Laarbeeklaan 101, 1090 Jette, Belgium In Vivo Cellular and Molecular Imaging 
      Laboratory (ICMI), Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel 
      (VUB), Jette, Belgium.
FAU - Remory, Isabel
AU  - Remory I
AD  - In Vivo Cellular and Molecular Imaging Laboratory (ICMI), Faculty of Medicine and 
      Pharmacy, Vrije Universiteit Brussel (VUB), Jette, Belgium.
FAU - De Raeve, Hendrik
AU  - De Raeve H
AD  - Department of Pathology, UZ Brussel, Jette, Belgium.
FAU - Tierens, Simon
AU  - Tierens S
AD  - In Vivo Cellular and Molecular Imaging Laboratory (ICMI), Faculty of Medicine and 
      Pharmacy, Vrije Universiteit Brussel (VUB), Jette, Belgium.
FAU - Hernot, Sophie
AU  - Hernot S
AD  - In Vivo Cellular and Molecular Imaging Laboratory (ICMI), Faculty of Medicine and 
      Pharmacy, Vrije Universiteit Brussel (VUB), Jette, Belgium.
FAU - Van Camp, Guy
AU  - Van Camp G
AD  - Department of Cardiology, Centrum Voor Hart-en Vaatziekten (CHVZ), UZ Brussel, 
      Laarbeeklaan 101, 1090 Jette, Belgium In Vivo Cellular and Molecular Imaging 
      Laboratory (ICMI), Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel 
      (VUB), Jette, Belgium.
FAU - Droogmans, Steven
AU  - Droogmans S
AD  - Department of Cardiology, Centrum Voor Hart-en Vaatziekten (CHVZ), UZ Brussel, 
      Laarbeeklaan 101, 1090 Jette, Belgium In Vivo Cellular and Molecular Imaging 
      Laboratory (ICMI), Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel 
      (VUB), Jette, Belgium.
FAU - Cosyns, Bernard
AU  - Cosyns B
AD  - Department of Cardiology, Centrum Voor Hart-en Vaatziekten (CHVZ), UZ Brussel, 
      Laarbeeklaan 101, 1090 Jette, Belgium In Vivo Cellular and Molecular Imaging 
      Laboratory (ICMI), Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel 
      (VUB), Jette, Belgium.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140430
PL  - England
TA  - Eur Heart J Cardiovasc Imaging
JT  - European heart journal. Cardiovascular Imaging
JID - 101573788
RN  - Aortic Valve, Calcification of
SB  - IM
MH  - Animals
MH  - Aortic Valve/diagnostic imaging/*pathology
MH  - Aortic Valve Stenosis/*diagnostic imaging
MH  - Calcinosis/*diagnostic imaging
MH  - Diagnosis, Differential
MH  - Disease Models, Animal
MH  - Echocardiography/*methods
MH  - Heart Valve Diseases/*diagnostic imaging
MH  - Male
MH  - Rats
MH  - Rats, Wistar
OTO - NOTNLM
OT  - Cardiovascular calcifications
OT  - Echocardiography
OT  - Integrated backscatter
OT  - Small animals
EDAT- 2014/05/03 06:00
MHDA- 2015/05/12 06:00
CRDT- 2014/05/03 06:00
PHST- 2014/05/03 06:00 [entrez]
PHST- 2014/05/03 06:00 [pubmed]
PHST- 2015/05/12 06:00 [medline]
AID - jeu067 [pii]
AID - 10.1093/ehjci/jeu067 [doi]
PST - ppublish
SO  - Eur Heart J Cardiovasc Imaging. 2014 Sep;15(9):1042-7. doi: 10.1093/ehjci/jeu067. 
      Epub 2014 Apr 30.