PMID- 24788205
OWN - NLM
STAT- MEDLINE
DCOM- 20150205
LR  - 20211021
IS  - 1573-9368 (Electronic)
IS  - 0962-8819 (Linking)
VI  - 23
IP  - 4
DP  - 2014 Aug
TI  - A pre-breeding screening program for transgenic boars based on fluorescence in 
      situ hybridization assay.
PG  - 679-89
LID - 10.1007/s11248-014-9801-4 [doi]
AB  - For efficient transgenic herd expansion, only the transgenic animals that possess 
      the ability to transmit transgene into next generation are considered for 
      breeding. However, for transgenic pig, practically lacking a pre-breeding 
      screening program, time, labor and money is always wasted to maintain 
      non-transgenic pigs, low or null transgenic transmission pigs and the related 
      fruitless gestations. Developing a pre-breeding screening program would make the 
      transgenic herd expansion more economical and efficient. In this technical 
      report, we proposed a three-step pre-breeding screening program for transgenic 
      boars simply through combining the fluorescence in situ hybridization (FISH) 
      assay with the common pre-breeding screening workflow. In the first step of 
      screening, combined with general transgenic phenotype analysis, FISH is used to 
      identify transgenic boars. In the second step of screening, combined with 
      conventional semen test, FISH is used to detect transgenic sperm, thus to 
      identify the individuals producing high quality semen and transgenic sperm. In 
      the third step of screening, FISH is used to assess the in vitro fertilization 
      embryos, thus finally to identify the individuals with the ability to produce 
      transgenic embryos. By this three-step screening, the non-transgenic boars and 
      boars with no ability to produce transgenic sperm or transgenic embryos would be 
      eliminated; therefore only those boars could produce transgenic offspring are 
      maintained and used for breeding and herd expansion. It is the first time a 
      systematic pre-breeding screening program is proposed for transgenic pigs. This 
      program might also be applied in other transgenic large animals, and provide an 
      economical and efficient strategy for herd expansion.
FAU - Bou, Gerelchimeg
AU  - Bou G
AD  - The College of Life Science, Northeast Agricultural University, Harbin, 150030, 
      People's Republic of China.
FAU - Sun, Mingju
AU  - Sun M
FAU - Lv, Ming
AU  - Lv M
FAU - Zhu, Jiang
AU  - Zhu J
FAU - Li, Hui
AU  - Li H
FAU - Wang, Juan
AU  - Wang J
FAU - Li, Lu
AU  - Li L
FAU - Liu, Zhongfeng
AU  - Liu Z
FAU - Zheng, Zhong
AU  - Zheng Z
FAU - He, Wenteng
AU  - He W
FAU - Kong, Qingran
AU  - Kong Q
FAU - Liu, Zhonghua
AU  - Liu Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140501
PL  - Netherlands
TA  - Transgenic Res
JT  - Transgenic research
JID - 9209120
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified/*genetics/growth & development
MH  - *Breeding
MH  - DNA/analysis/genetics
MH  - Embryo, Mammalian/cytology/*metabolism
MH  - Fertilization in Vitro/*veterinary
MH  - Green Fluorescent Proteins/*genetics/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Real-Time Polymerase Chain Reaction
MH  - Spermatozoa/*chemistry
MH  - Swine
MH  - Transgenes/*physiology
EDAT- 2014/05/03 06:00
MHDA- 2015/02/06 06:00
CRDT- 2014/05/03 06:00
PHST- 2013/12/21 00:00 [received]
PHST- 2014/04/17 00:00 [accepted]
PHST- 2014/05/03 06:00 [entrez]
PHST- 2014/05/03 06:00 [pubmed]
PHST- 2015/02/06 06:00 [medline]
AID - 10.1007/s11248-014-9801-4 [doi]
PST - ppublish
SO  - Transgenic Res. 2014 Aug;23(4):679-89. doi: 10.1007/s11248-014-9801-4. Epub 2014 
      May 1.