PMID- 24789168
OWN - NLM
STAT- MEDLINE
DCOM- 20140819
LR  - 20211203
IS  - 1861-0293 (Electronic)
IS  - 1340-3443 (Linking)
VI  - 68
IP  - 3
DP  - 2014 Jul
TI  - Anethole and eugenol reduce in vitro and in vivo leukocyte migration induced by 
      fMLP, LTB4, and carrageenan.
PG  - 567-75
LID - 10.1007/s11418-014-0839-7 [doi]
AB  - The aim of this study was to investigate the effect of anethole (AN) and eugenol 
      (EUG) on leukocyte migration using in vitro chemotaxis and in situ 
      microcirculation assays. BALB/c mice were used for the in vitro chemotaxis assay, 
      and Wistar rats for the in situ microcirculation assay. We evaluated (a) the in 
      vitro leukocyte migration in response to chemotactic factors 
      (formyl-methionyl-leucyl-phenylalanine [fMLP] and leukotriene B4 [LTB4]) and (b) 
      the rolling, adhesion, and migration of leukocytes induced by an injection of 
      carrageenan (100 microg/cavity) into the scrotum of the animal. In the in vitro 
      chemotaxis assay, AN and EUG at doses of 1, 3, 9, and 27 microg/ml significantly 
      inhibited leukocyte migration when stimulated by the chemotactic agents fMLP and 
      LTB4. In the in situ microcirculation assay, AN at doses of 125 and 250 mg/kg and 
      EUG at a dose of 250 mg/kg significantly decreased the number of leukocytes that 
      rolled, adhered, and migrated to perivascular tissue. The results indicate that 
      AN and EUG exert inhibitory effects on leukocyte migration, highlighting their 
      possible use to diminish excessive leukocyte migration in the inflammatory 
      process.
FAU - Estevao-Silva, Camila Fernanda
AU  - Estevao-Silva CF
AD  - Laboratory of Inflammation, Department of Pharmacology and Therapeutic, State 
      University of Maringa, Maringa, PR, Brazil.
FAU - Kummer, Raquel
AU  - Kummer R
FAU - Fachini-Queiroz, Fernanda Carolina
AU  - Fachini-Queiroz FC
FAU - Grespan, Renata
AU  - Grespan R
FAU - Nogueira de Melo, Gessilda Alcantara
AU  - Nogueira de Melo GA
FAU - Baroni, Silmara
AU  - Baroni S
FAU - Cuman, Roberto Kenji Nakamura
AU  - Cuman RK
FAU - Bersani-Amado, Ciomar Aparecida
AU  - Bersani-Amado CA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140501
PL  - Japan
TA  - J Nat Med
JT  - Journal of natural medicines
JID - 101518405
RN  - 0 (Allylbenzene Derivatives)
RN  - 0 (Anisoles)
RN  - 1HGW4DR56D (Leukotriene B4)
RN  - 3T8H1794QW (Eugenol)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 9000-07-1 (Carrageenan)
RN  - Q3JEK5DO4K (anethole)
SB  - IM
MH  - Allylbenzene Derivatives
MH  - Animals
MH  - Anisoles/*pharmacology
MH  - Carrageenan/pharmacology
MH  - Chemotaxis, Leukocyte/*drug effects
MH  - Eugenol/*pharmacology
MH  - Humans
MH  - Leukocytes/drug effects/physiology
MH  - Leukotriene B4/pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Rats
MH  - Rats, Wistar
EDAT- 2014/05/03 06:00
MHDA- 2014/08/20 06:00
CRDT- 2014/05/03 06:00
PHST- 2013/05/22 00:00 [received]
PHST- 2014/04/03 00:00 [accepted]
PHST- 2014/05/03 06:00 [entrez]
PHST- 2014/05/03 06:00 [pubmed]
PHST- 2014/08/20 06:00 [medline]
AID - 10.1007/s11418-014-0839-7 [doi]
PST - ppublish
SO  - J Nat Med. 2014 Jul;68(3):567-75. doi: 10.1007/s11418-014-0839-7. Epub 2014 May 
      1.