PMID- 2479110
OWN - NLM
STAT- MEDLINE
DCOM- 19891214
LR  - 20190908
IS  - 0148-5717 (Print)
IS  - 0148-5717 (Linking)
VI  - 16
IP  - 3
DP  - 1989 Jul-Sep
TI  - Comparison of direct fluorescent antibody, acridine orange, wet mount, and 
      culture for detection of Trichomonas vaginalis in women attending a public 
      sexually transmitted diseases clinic.
PG  - 127-31
AB  - To define the performance characteristics of two newer tests for Trichomonas 
      vaginalis (TV), the authors compared direct fluorescent antibody (DFA) (mixed 
      monoclonal antibody, Integrated Diagnostics, Inc, Berkeley, CA) and acridine 
      orange (AO) tests to standard wet mount (WM) preparations and culture (modified 
      Diamond medium) of vaginal wash specimens in consecutively examined women 
      presenting to a public sexually transmitted diseases (STD) clinic. Cultures for 
      Neisseria gonorrhoeae (GC), Chlamydia trachomatis (CT), and yeast were also 
      performed on all patients. Of 104 women, 59 (57%) were infected with one or more 
      pathogens. Trichomonas vaginalis was detected by WM and/or culture in 38 (37%) 
      women and was the most prevalent infection. Of the 38 patients with TV, 95% were 
      detected by culture, 83% by DFA, 66% by AO, and 66% by WM. An additional patient 
      was DFA positive but negative for TV by all other methods. The sensitivity of DFA 
      was superior to AO and WM in women with TV infection alone (96% compared to 67% 
      and 53%, respectively). It was comparable to AO and WM in women with multiple 
      infections (67% compared to 53% and 73%). Even in the presence of other 
      pathogens, DFA appears to be a reasonable alternative to culture for detection of 
      TV. In addition, DFA is rapid, easy to perform, and relatively inexpensive.
FAU - Bickley, L S
AU  - Bickley LS
AD  - Department of Medicine, University of Rochester School of Medicine, New York 
      14642.
FAU - Krisher, K K
AU  - Krisher KK
FAU - Punsalang, A Jr
AU  - Punsalang A Jr
FAU - Trupei, M A
AU  - Trupei MA
FAU - Reichman, R C
AU  - Reichman RC
FAU - Menegus, M A
AU  - Menegus MA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Sex Transm Dis
JT  - Sexually transmitted diseases
JID - 7705941
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Predictive Value of Tests
MH  - Sexually Transmitted Diseases/*diagnosis
MH  - Staining and Labeling
MH  - Trichomonas Vaginitis/*diagnosis
MH  - Trichomonas vaginalis/*isolation & purification
MH  - Vagina/*microbiology
EDAT- 1989/07/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1989/07/01 00:00
PHST- 1989/07/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1989/07/01 00:00 [entrez]
AID - 10.1097/00007435-198907000-00002 [doi]
PST - ppublish
SO  - Sex Transm Dis. 1989 Jul-Sep;16(3):127-31. doi: 10.1097/00007435-198907000-00002.