PMID- 2479123
OWN - NLM
STAT- MEDLINE
DCOM- 19891221
LR  - 20190727
IS  - 0041-008X (Print)
IS  - 0041-008X (Linking)
VI  - 101
IP  - 2
DP  - 1989 Nov
TI  - Effects of the beta-isomer of hexachlorocyclohexane on estrogen-sensitive human 
      mammary tumor cells.
PG  - 310-8
AB  - The effects of the beta-isomer of hexachlorocyclohexane (beta-HCH) on the 
      induction of the cytosolic progesterone receptor (PgRc), on the redistribution of 
      the estrogen receptor (ER), and its affinity for ER were investigated in the 
      estrogen-sensitive human mammary tumor cell line MCF-7. The effects of beta-HCH 
      were compared to those of estradiol-17 beta (E2) and 2,4,6-trichlorophenol (TCP), 
      a major urinary metabolite of beta-HCH in rats. beta-HCH in concentrations higher 
      than 1 microM caused induction of PgRc whereas TCP was essentially without effect 
      up to a cytotoxic concentration of 100 microM. Furthermore, beta-HCH (10 microM) 
      caused redistribution of ER, i.e., decrease of cytosolic ER concentration and 
      increase of nuclear ER concentration, in a way similar to E2. In contrast to 
      this, beta-HCH up to a molar excess of 6 X 10(4) (concentration 30 microM) caused 
      no significant displacement of [3H]E2 from ER indicating that beta-HCH has no 
      substantial affinity for ER. It is concluded that beta-HCH has estrogenic 
      properties which are at variance with the failure to demonstrate binding of 
      beta-HCH to ER.
FAU - Coosen, R
AU  - Coosen R
AD  - Laboratory of Toxicology, National Institute of Public Health and Environmental 
      Protection, Bilthoven, The Netherlands.
FAU - van Velsen, F L
AU  - van Velsen FL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Toxicol Appl Pharmacol
JT  - Toxicology and applied pharmacology
JID - 0416575
RN  - 0 (Chlorophenols)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Receptors, Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 59NEE7PCAB (Hexachlorocyclohexane)
RN  - MHS8C5BAUZ (2,4,6-trichlorophenol)
RN  - YM80ODM9PD (beta-hexachlorocyclohexane)
SB  - IM
MH  - Animals
MH  - Biotransformation/drug effects
MH  - Breast Neoplasms/analysis/*metabolism
MH  - Cell Nucleus/drug effects/metabolism
MH  - Chlorophenols/pharmacology
MH  - Cytosol/drug effects/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Estradiol/pharmacology
MH  - Female
MH  - Hexachlorocyclohexane/*pharmacology
MH  - Humans
MH  - Rats
MH  - Receptors, Estrogen/*metabolism
MH  - Receptors, Progesterone/drug effects/metabolism
MH  - Tumor Cells, Cultured/drug effects
EDAT- 1989/11/01 00:00
MHDA- 1989/11/01 00:01
CRDT- 1989/11/01 00:00
PHST- 1989/11/01 00:00 [pubmed]
PHST- 1989/11/01 00:01 [medline]
PHST- 1989/11/01 00:00 [entrez]
AID - 10.1016/0041-008x(89)90279-2 [doi]
PST - ppublish
SO  - Toxicol Appl Pharmacol. 1989 Nov;101(2):310-8. doi: 10.1016/0041-008x(89)90279-2.