PMID- 24794127
OWN - NLM
STAT- MEDLINE
DCOM- 20150330
LR  - 20181202
IS  - 1873-5894 (Electronic)
IS  - 0730-725X (Linking)
VI  - 32
IP  - 7
DP  - 2014 Sep
TI  - Analysis of feasibility of in vitro nuclear magnetic resonance tracking human 
      umbilical cord mesenchymal stem cells by Gd-DTPA labeled.
PG  - 934-40
LID - S0730-725X(14)00051-4 [pii]
LID - 10.1016/j.mri.2014.02.008 [doi]
AB  - OBJECTIVE: Three different kinds of transfection reagents were used to mediate 
      the transfection of gadolinium-diethylenetriamine penta-acetic acid (Gd-DTPA) 
      into human umbilical-cord-derived mesenchymal stem cells (hUCMSCs). The efficacy 
      of different transfection reagents and the feasibility of NMR tracer in vitro of 
      magnetized stem cells were estimated. METHODS: After purification by tissue 
      explants adherent method, the biological characteristics of hUCMSCs in vitro were 
      identified by subculture and amplification. Calcium phosphate, Effectene and 
      liposome2000 were used to transfect Gd-DTPA-labeled hUCMSCs respectively, and 
      cell counting was used to mediate the transfection of Gd-DTPA into hUCMSCs, which 
      were then induced to lipoblast and osteoblast in vitro. The determination of the 
      transfection activities of the transfection reagents was conducted by measuring 
      the magnetic resonance imaging (MRI) signal intensity of the Gd-DTPA-labeled 
      cells and the concentration of gadolinium ion in the cells. Furthermore, the 
      relationship between the signal intensity of Gd-DTPA-labeled hUCMSCsMRI, cell 
      subculture and generations was studied. RESULTS: Primary cells were obtained by 
      tissue explants adherent for two weeks. The cells displayed a long spindle form 
      and grew in swirl. After two passage generations, the cellular morphology became 
      more homogeneous. The result detected by the flow cytometer showed that CD29C, 
      D44, CD90, and CD105 were highly expressed, while no CD45, CD40, and HLA-DR 
      expression was detected in the third generation cells. Directional induction in 
      vitro caused the differentiation into lipoblast and osteoblast. After transfected 
      by calcium phosphate, Effectene and liposome 2000, the signal intensity of stem 
      cells was 2281.2+/-118.8, 2031.9+/-59.7 and 1887.4+/-40.8 measured by MRI. Differences 
      between these three groups were statistically significant (P<0.05). The 
      concentrations of gadolinium ion in three groups of stem cells were 
      0.178+/-0.009mg/L, 0.158+/-0.003mg/L and 0.120+/-0.002mg/L respectively, examined by 
      inductively coupled plasma atomic emission spectrometry. No significant 
      differences were found among these three groups (P<0.05). The proliferation and 
      differentiation abilities of the Gd-DTPA-labeled stem cells were not affected. A 
      minimum 5x10(4) Gd-DTPA-labeled stem cells could be traced with MRI in vitro and 
      presented in high signal. The trace duration time in vitro was about 12days. 
      CONCLUSIONS: Tissue explants adherent method can be availably applied to purify 
      hUCMSCs. The Effectene method was proved to have the best transfection effect. 
      The proliferation ability and differentiation potency of Gd-DTPA-labeled hUCMSCs 
      were not affected, and the NMR of labeled stem cells in vitro was proved to be 
      feasible.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Shuai, Han-Lin
AU  - Shuai HL
AD  - Department of Obstetrics and Gynecology, the First Affiliated Hospital of Jinan 
      University, Guangzhou, 510630, China.
FAU - Yan, Rui-Ling
AU  - Yan RL
AD  - Department of Obstetrics and Gynecology, the First Affiliated Hospital of Jinan 
      University, Guangzhou, 510630, China.
FAU - Song, Hong
AU  - Song H
AD  - Department of Obstetrics and Gynecology, the First Affiliated Hospital of Jinan 
      University, Guangzhou, 510630, China.
FAU - Chen, Dan-Liang
AU  - Chen DL
AD  - Department of Obstetrics and Gynecology, the First Affiliated Hospital of Jinan 
      University, Guangzhou, 510630, China.
FAU - Luo, Xin
AU  - Luo X
AD  - Department of Obstetrics and Gynecology, the First Affiliated Hospital of Jinan 
      University, Guangzhou, 510630, China. Electronic address: luoxin121@yeah.net.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140225
PL  - Netherlands
TA  - Magn Reson Imaging
JT  - Magnetic resonance imaging
JID - 8214883
RN  - 0 (Contrast Media)
RN  - K2I13DR72L (Gadolinium DTPA)
SB  - IM
MH  - Cell Tracking/*methods
MH  - Cells, Cultured
MH  - Contrast Media
MH  - Cord Blood Stem Cell Transplantation/methods
MH  - Feasibility Studies
MH  - Fetal Blood/*cytology
MH  - *Gadolinium DTPA/pharmacokinetics
MH  - Humans
MH  - Magnetic Resonance Imaging/*methods
MH  - Magnetic Resonance Spectroscopy/*methods
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Staining and Labeling/methods
MH  - Transfection/methods
OTO - NOTNLM
OT  - Gd-DTPA
OT  - Magnetic resonance imaging (MRI)
OT  - Mesenchymal stem cell
OT  - Trace
EDAT- 2014/05/06 06:00
MHDA- 2015/03/31 06:00
CRDT- 2014/05/06 06:00
PHST- 2013/11/27 00:00 [received]
PHST- 2014/01/27 00:00 [revised]
PHST- 2014/02/03 00:00 [accepted]
PHST- 2014/05/06 06:00 [entrez]
PHST- 2014/05/06 06:00 [pubmed]
PHST- 2015/03/31 06:00 [medline]
AID - S0730-725X(14)00051-4 [pii]
AID - 10.1016/j.mri.2014.02.008 [doi]
PST - ppublish
SO  - Magn Reson Imaging. 2014 Sep;32(7):934-40. doi: 10.1016/j.mri.2014.02.008. Epub 
      2014 Feb 25.