PMID- 24794181 OWN - NLM STAT- MEDLINE DCOM- 20150212 LR - 20220129 IS - 1477-2566 (Electronic) IS - 1465-3249 (Print) IS - 1465-3249 (Linking) VI - 16 IP - 7 DP - 2014 Jul TI - Comparative analysis of multilineage properties of mesenchymal stromal cells derived from fetal sources shows an advantage of mesenchymal stromal cells isolated from cord blood in chondrogenic differentiation potential. PG - 893-905 LID - S1465-3249(14)00454-X [pii] LID - 10.1016/j.jcyt.2014.02.008 [doi] AB - BACKGROUND AIMS: Cord blood (CB) and amniotic fluid (AF) could represent new and attractive mesenchymal stromal cell (MSC) sources, but their potential therapeutic applications are still limited by lack of standardized protocols for isolation and differentiation. In particular, chondrogenic differentiation has never been deeply investigated. METHODS: MSCs were obtained from CB and AF samples collected during cesarean sections at term and compared for their biological and differentiation properties, with particular interest in cartilage differentiation, in which quantitative real-time polymerase chain reaction and immunohistochemical analyses were performed to evaluate the expression of type 2 collagen, type 10 collagen, SRY-box9 and aggrecan. RESULTS: We were able to isolate MSCs from 12 of 30 (40%) and 5 of 20 (25%) CB and AF units, respectively. Fluorescence in situ hybridization analysis indicated the fetal origin of isolated MSC strains. Both populations expressed mesenchymal but not endothelial and hematopoietic markers, even though we observed a lower expression of human leukocyte antigen (HLA) I in CB-MSCs. No differences in proliferation rate and cell cycle analysis could be detected. After osteogenic induction, both populations showed matrix mineralization and typical marker expression. Under chondrogenic conditions, pellets derived from CB-MSCs, in contrast with AF-MSCs pellets, were significantly larger, showed cartilage-like morphology and resulted positive for chondrocyte-associated markers, such as type 2 collagen, type 10 collagen, SRY-box9 and aggrecan. CONCLUSIONS: Our results show that CB-MSCs and AF-MSCs collected at term differ from each other in their biological and differentiation properties. In particular, only CB-MSCs showed a clear chondrogenic potential and thus could represent an ideal candidate for cartilage-tissue engineering. CI - Copyright (c) 2014 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved. FAU - Pievani, Alice AU - Pievani A AD - Dulbecco Telethon Institute at Tettamanti Research Center, Pediatric Department, University of Milano-Bicocca, Monza, Italy; Tettamanti Research Center, Pediatric Department, University of Milano-Bicocca, San Gerardo Hospital, Monza, Italy. FAU - Scagliotti, Valeria AU - Scagliotti V AD - Dulbecco Telethon Institute at Tettamanti Research Center, Pediatric Department, University of Milano-Bicocca, Monza, Italy; Tettamanti Research Center, Pediatric Department, University of Milano-Bicocca, San Gerardo Hospital, Monza, Italy. FAU - Russo, Francesca Maria AU - Russo FM AD - Department of Obstetrics and Gynecology, University of Milano-Bicocca, Monza, Italy. FAU - Azario, Isabella AU - Azario I AD - Dulbecco Telethon Institute at Tettamanti Research Center, Pediatric Department, University of Milano-Bicocca, Monza, Italy; Tettamanti Research Center, Pediatric Department, University of Milano-Bicocca, San Gerardo Hospital, Monza, Italy. FAU - Rambaldi, Benedetta AU - Rambaldi B AD - Tettamanti Research Center, Pediatric Department, University of Milano-Bicocca, San Gerardo Hospital, Monza, Italy. FAU - Sacchetti, Benedetto AU - Sacchetti B AD - Department of Molecular Medicine, Sapienza University, Rome, Italy. FAU - Marzorati, Simona AU - Marzorati S AD - Dulbecco Telethon Institute at Tettamanti Research Center, Pediatric Department, University of Milano-Bicocca, Monza, Italy; Tettamanti Research Center, Pediatric Department, University of Milano-Bicocca, San Gerardo Hospital, Monza, Italy. FAU - Erba, Eugenio AU - Erba E AD - Department of Oncology, Flow Cytometry Unit, IRCCS Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy. FAU - Giudici, Giovanni AU - Giudici G AD - Tettamanti Research Center, Pediatric Department, University of Milano-Bicocca, San Gerardo Hospital, Monza, Italy. FAU - Riminucci, Mara AU - Riminucci M AD - Department of Molecular Medicine, Sapienza University, Rome, Italy. FAU - Biondi, Andrea AU - Biondi A AD - Pediatric Department, University of Milano-Bicocca, Fondazione MBBM/San Gerardo Hospital, Monza, Italy. FAU - Vergani, Patrizia AU - Vergani P AD - Department of Obstetrics and Gynecology, University of Milano-Bicocca, Monza, Italy. FAU - Serafini, Marta AU - Serafini M AD - Dulbecco Telethon Institute at Tettamanti Research Center, Pediatric Department, University of Milano-Bicocca, Monza, Italy; Tettamanti Research Center, Pediatric Department, University of Milano-Bicocca, San Gerardo Hospital, Monza, Italy. Electronic address: serafinim72@gmail.com. LA - eng GR - TCP07004/TI_/Telethon/Italy PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140501 PL - England TA - Cytotherapy JT - Cytotherapy JID - 100895309 SB - IM MH - Cell Differentiation/*genetics MH - Cell Lineage/genetics MH - Chondrogenesis/*genetics MH - Female MH - Fetal Blood/*cytology MH - Fetus MH - Humans MH - In Situ Hybridization, Fluorescence MH - Mesenchymal Stem Cells/*cytology MH - Pregnancy MH - Tissue Engineering PMC - PMC4062948 OTO - NOTNLM OT - amniotic fluid OT - chondrogenic differentiation OT - cord blood OT - mesenchymal stromal cells EDAT- 2014/05/06 06:00 MHDA- 2015/02/13 06:00 PMCR- 2014/07/01 CRDT- 2014/05/06 06:00 PHST- 2013/06/25 00:00 [received] PHST- 2014/01/29 00:00 [revised] PHST- 2014/02/09 00:00 [accepted] PHST- 2014/05/06 06:00 [entrez] PHST- 2014/05/06 06:00 [pubmed] PHST- 2015/02/13 06:00 [medline] PHST- 2014/07/01 00:00 [pmc-release] AID - S1465-3249(14)00454-X [pii] AID - 10.1016/j.jcyt.2014.02.008 [doi] PST - ppublish SO - Cytotherapy. 2014 Jul;16(7):893-905. doi: 10.1016/j.jcyt.2014.02.008. Epub 2014 May 1.