PMID- 24794875
OWN - NLM
STAT- MEDLINE
DCOM- 20150520
LR  - 20141201
IS  - 1876-7478 (Electronic)
IS  - 1874-7787 (Linking)
VI  - 18 Pt A
DP  - 2014 Dec
TI  - Cloning, expression and methylation analysis of piwil2 in half-smooth tongue sole 
      (Cynoglossus semilaevis).
PG  - 45-54
LID - S1874-7787(14)00043-9 [pii]
LID - 10.1016/j.margen.2014.04.004 [doi]
AB  - piwi is an important regulator gene in germ cell division during spermatogenesis. 
      piwi homologous genes are involved in gametogenesis and germline specification, 
      and knocking down these genes could affect germ cell meiotic progression. To 
      understand the function of piwi-related genes in spermatogenesis, we cloned a 
      Piwi-subfamily member (piwil2 gene) from the gonad of Cynoglossus semilaevis. The 
      full-length of piwil2 cDNA was 3314bp, including a 3162bp open reading frame 
      (ORF), a 60bp 5'-UTR, along with a 92bp 3'-UTR, and encoded a predicted protein 
      of 1053 amino acid residues. Phylogenetic analysis showed that the PIWIL2 
      putative protein belonged to the Argonaute protein family, and Piwi-subfamily, 
      with typical PAZ and Piwi domains. Ultrathin sections of different gonadal 
      stages, and real-time quantitative PCR showed that the relative expression of the 
      piwil2 gene couldn't be detected until day 95 (95days) larvae, when germ cell 
      divided rapidly in C. semilaevis. The piwil2 transcripts were more abundant in 
      gonads of males and neo-males than in females, and weakly expressed in other 
      tissues and organs. Compared with the relative expression of piwil2 in gonads, 
      the CpG methylation levels were significantly higher in females. Chromosomal 
      fluorescence in situ hybridization (FISH) showed that the piwil2 gene was located 
      on the Z sex chromosome of C. semilaevis. These results suggest that piwil2 plays 
      an important role in spermatogenesis of C. semilaevis.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Zhang, Liyan
AU  - Zhang L
AD  - College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, 
      China; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Qingdao 266071, China.
FAU - Liu, Wanjun
AU  - Liu W
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao 266071, China; Fisheries College, Ocean University of China, 5 Yushan 
      Road, Qingdao 266003, China.
FAU - Shao, Changwei
AU  - Shao C
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao 266071, China.
FAU - Zhang, Ning
AU  - Zhang N
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao 266071, China.
FAU - Li, Hailong
AU  - Li H
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao 266071, China.
FAU - Liu, Kun
AU  - Liu K
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao 266071, China.
FAU - Dong, Zhongdian
AU  - Dong Z
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao 266071, China; Fisheries College, Ocean University of China, 5 Yushan 
      Road, Qingdao 266003, China.
FAU - Qi, Qian
AU  - Qi Q
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao 266071, China; Fisheries College, Ocean University of China, 5 Yushan 
      Road, Qingdao 266003, China.
FAU - Zhao, Wen
AU  - Zhao W
AD  - College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, 
      China.
FAU - Chen, Songlin
AU  - Chen S
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao 266071, China. Electronic address: chensl@ysfri.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140501
PL  - Netherlands
TA  - Mar Genomics
JT  - Marine genomics
JID - 101475200
RN  - 0 (Argonaute Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Argonaute Proteins/chemistry/*genetics/*metabolism
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - *DNA Methylation
MH  - Female
MH  - Flatfishes/classification/*genetics/growth & development/metabolism
MH  - *Gene Expression Regulation
MH  - Gonads/cytology
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Phylogeny
MH  - Sex Chromosomes/genetics
MH  - Spermatogenesis/genetics
OTO - NOTNLM
OT  - Histological observations
OT  - Phylogenetic analysis
OT  - RACE
OT  - RT-qPCR
OT  - Spermatogenesis
EDAT- 2014/05/06 06:00
MHDA- 2015/05/21 06:00
CRDT- 2014/05/06 06:00
PHST- 2014/01/26 00:00 [received]
PHST- 2014/04/17 00:00 [revised]
PHST- 2014/04/18 00:00 [accepted]
PHST- 2014/05/06 06:00 [entrez]
PHST- 2014/05/06 06:00 [pubmed]
PHST- 2015/05/21 06:00 [medline]
AID - S1874-7787(14)00043-9 [pii]
AID - 10.1016/j.margen.2014.04.004 [doi]
PST - ppublish
SO  - Mar Genomics. 2014 Dec;18 Pt A:45-54. doi: 10.1016/j.margen.2014.04.004. Epub 
      2014 May 1.