PMID- 24795053
OWN - NLM
STAT- MEDLINE
DCOM- 20141217
LR  - 20140505
IS  - 1550-8080 (Electronic)
IS  - 0091-7370 (Linking)
VI  - 44
IP  - 2
DP  - 2014 Spring
TI  - Tert-butylhydroquinone protects the spinal cord against inflammatory response 
      produced by spinal cord injury.
PG  - 151-7
AB  - Antioxidant transcription factor nuclear factor erythroid 2-related factor 2 
      (Nrf2) has been shown in our previous studies to play an important role in 
      protection against spinal cord injury (SCI) induced inflammatory response. The 
      objective of this study was to test whether tert-butylhydroquinone (tBHQ), a 
      novel Nrf2 activator, can protect the spinal cord against SCI-induced 
      inflammatory damage. Adult male Sprague-Dawley rats were subjected to laminectomy 
      at T8-T9 and compression with a vascular clip. Three groups were analyzed: a sham 
      group, a SCI group, and a SCI+rhEPO group (n=16 per group). We measured Nrf2 and 
      nuclear factor kappa B (NF-kappaB) binding activities by an electrophoretic mobility 
      shift assay (EMSA). We also measured the concentrations of tumor necrosis 
      factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and interleukin-6 (IL-6) by an 
      enzyme-linked immunosorbent assay (ELISA); we also measured hindlimb locomotion 
      function by the Basso, Beattie, and Bresnahan (BBB) rating, spinal cord edema by 
      wet/dry weight method, and apoptosis by terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) analysis. The results showed 
      that the induction of the Nrf2 activity by tBHQ markedly decreased NF-kappaB 
      activation and inflammatory cytokines production in the injured spinal cord. 
      Administration of tBHQ also significantly attenuated SCI induced hindlimb 
      locomotion deficits, spinal cord edema, and apoptosis. To conclude, pre-treatment 
      with tBHQ could attenuate the spinal cord inflammatory response after SCI.
FAU - Jin, Wei
AU  - Jin W
AD  - Department of Neurosurgery, Drum Tower Hospital, Medical School of Nanjing 
      University, 321 Zhongshan Road, Nanjing 210008, Jiangsu Province, China; phone: 
      86 25 83106666; njneirosurgery@163.com.
FAU - Ni, Hongbin
AU  - Ni H
FAU - Hou, Xiaoshan
AU  - Hou X
FAU - Ming, Xing
AU  - Ming X
FAU - Wang, Jing
AU  - Wang J
FAU - Yuan, Baoyu
AU  - Yuan B
FAU - Zhu, Tiansheng
AU  - Zhu T
FAU - Jiang, Jian
AU  - Jiang J
FAU - Wang, Handong
AU  - Wang H
FAU - Liang, Weibang
AU  - Liang W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Ann Clin Lab Sci
JT  - Annals of clinical and laboratory science
JID - 0410247
RN  - 0 (Cytokines)
RN  - 0 (Hydroquinones)
RN  - 0 (Inflammation Mediators)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NF-kappa B)
RN  - 0 (Neuroprotective Agents)
RN  - 9007-49-2 (DNA)
RN  - C12674942B (2-tert-butylhydroquinone)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Blood-Brain Barrier/drug effects/pathology
MH  - Cytokines/metabolism
MH  - DNA/metabolism
MH  - Disease Models, Animal
MH  - Edema/complications/drug therapy/pathology/physiopathology
MH  - Hindlimb/drug effects/physiopathology
MH  - Hydroquinones/pharmacology/*therapeutic use
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Inflammation/*complications/*drug therapy/pathology
MH  - Inflammation Mediators/metabolism
MH  - Male
MH  - Motor Activity/drug effects
MH  - NF-E2-Related Factor 2/metabolism
MH  - NF-kappa B/metabolism
MH  - Neuroprotective Agents/pharmacology/*therapeutic use
MH  - Protein Binding/drug effects
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord/drug effects/*pathology/physiopathology/surgery
MH  - Spinal Cord Injuries/*complications/*drug therapy/physiopathology
OTO - NOTNLM
OT  - Nrf2
OT  - inflammatory response
OT  - spinal cord injury
OT  - tBHQ
EDAT- 2014/05/06 06:00
MHDA- 2014/12/18 06:00
CRDT- 2014/05/06 06:00
PHST- 2014/05/06 06:00 [entrez]
PHST- 2014/05/06 06:00 [pubmed]
PHST- 2014/12/18 06:00 [medline]
AID - 44/2/151 [pii]
PST - ppublish
SO  - Ann Clin Lab Sci. 2014 Spring;44(2):151-7.