PMID- 2479573
OWN - NLM
STAT- MEDLINE
DCOM- 19900103
LR  - 20190624
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 168
IP  - 3
DP  - 1989 Sep 22
TI  - The autoradiographic distribution of substance P binding sites in guinea-pig vas 
      deferens is altered by capsaicin pretreatment.
PG  - 337-45
AB  - The distribution of binding sites for [125I]Bolton-Hunter substance P (BHSP) was 
      investigated in vasa deferentia from normal, capsaicin-pretreated and 
      vehicle-pretreated guinea-pigs, using qualitative and quantitative 
      autoradiography. Dense binding of BHSP was seen over the outer longitudinal 
      muscle with less over the inner longitudinal muscle. Very low specific binding 
      occurred to the circular muscle and was absent in the mucosa. Characterization in 
      slide-mounted sections showed that binding was saturable and of high affinity, 
      with equilibrium dissociation constant (KD) 91 +/- 15 pM. BHSP was displaced by 
      substance P greater than neurokinin A greater than neurokinin B, suggesting 
      binding to NK-1 receptors. Capsaicin pretreatment had no effect on the lengthwise 
      distribution of binding sites but significantly altered their relative 
      distribution between the different smooth muscle layers. There was a very marked 
      increase in BHSP binding over the inner longitudinal muscle and the inner part of 
      the circular muscle layer, whereas binding was virtually abolished over the outer 
      longitudinal muscle, compared with vehicle control. Capsaicin-sensitive binding 
      sites over the outer longitudinal muscle may be located presynaptically on 
      capsaicin-susceptible primary afferent sensory neurons. In contrast, the increase 
      in number of binding sites associated with the inner longitudinal muscle may be 
      due to receptor upregulation resulting from loss of sensory innervation, and 
      suggests that these binding sites are postsynaptic.
FAU - Mussap, C J
AU  - Mussap CJ
AD  - Department of Biological Sciences, Deakin University, Vic., Australia.
FAU - Lew, R
AU  - Lew R
FAU - Burcher, E
AU  - Burcher E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Receptors, Neurokinin-1)
RN  - 0 (Receptors, Neurotransmitter)
RN  - 33507-63-0 (Substance P)
RN  - S07O44R1ZM (Capsaicin)
SB  - IM
MH  - Animals
MH  - Autoradiography
MH  - Capsaicin/*pharmacology
MH  - Guinea Pigs
MH  - In Vitro Techniques
MH  - Male
MH  - Muscle Contraction/drug effects
MH  - Muscle, Smooth/drug effects/*metabolism
MH  - Receptors, Neurokinin-1
MH  - Receptors, Neurotransmitter/*metabolism
MH  - Substance P/*metabolism
MH  - Vas Deferens/anatomy & histology/drug effects/metabolism
EDAT- 1989/09/22 00:00
MHDA- 1989/09/22 00:01
CRDT- 1989/09/22 00:00
PHST- 1989/09/22 00:00 [pubmed]
PHST- 1989/09/22 00:01 [medline]
PHST- 1989/09/22 00:00 [entrez]
AID - 0014-2999(89)90795-4 [pii]
AID - 10.1016/0014-2999(89)90795-4 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 1989 Sep 22;168(3):337-45. doi: 10.1016/0014-2999(89)90795-4.