PMID- 24796744
OWN - NLM
STAT- MEDLINE
DCOM- 20141027
LR  - 20201209
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 30
IP  - 5
DP  - 2014 May
TI  - [The expression of C-type lectin-like domain of DNGR-1 molecules of Chinese 
      rhesus macaques in E.coli].
PG  - 489-92
AB  - OBJECTIVE: To obtain the nucleotide sequence and express the C-type lectin-like 
      domain (CTLD) of C-type lectin domain family 9, member A (Clec9A) gene of Chinese 
      rhesus macaques. METHODS: The complete coding region of rhesus macaque Clec9A 
      gene was amplified by sequence specific RT-PCR and sub-cloned into the pGEM-T 
      vector and sequenced. Recombinant plasmid pET-32a-CTLD was constructed to express 
      the CTLD region of CLEC9A in BL21 (DE3) cells under IPTG induction. The 
      recombinant protein was identified by SDS-PAGE and Western blotting. RESULTS: The 
      726 bp complete coding sequence of Clec9A gene was obtained. Sequencing and 
      sequence alignments showed that rhesus macaque Clec9A gene shared 95.0% and 73.1% 
      nucleotide identities and 91.7% and 57.3% amino acid identities with humans and 
      mice, respectively. In vitro expression of CTLD resulted in a protein with Mr; of 
      32 000. Western blotting revealed that the protein had the similar antigenicity 
      with human DNGR-1. CONCLUSION: The Clec9A gene of Chinese rhesus macaques is 
      highly similar to that of humans and could code a protein with the antigenicity 
      of human CTLD protein.
FAU - Yao, Wenrong
AU  - Yao W
AD  - National Center for AIDS/STD Control and Prevention, Chinese Center for Disease 
      Control and Prevention, Beijing 102206, China.
FAU - Yang, Guibo
AU  - Yang G
AD  - National Center for AIDS/STD Control and Prevention, Chinese Center for Disease 
      Control and Prevention, Beijing 102206, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (CLEC9a protein, human)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Receptors, Mitogen)
RN  - 0 (Recombinant Fusion Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Western
MH  - China
MH  - Escherichia coli/genetics
MH  - Gene Expression
MH  - Genetic Vectors/genetics
MH  - Humans
MH  - Lectins, C-Type/genetics/*metabolism
MH  - Macaca mulatta/genetics/*metabolism
MH  - Molecular Sequence Data
MH  - Receptors, Mitogen/genetics/*metabolism
MH  - Recombinant Fusion Proteins/*metabolism
MH  - Sequence Homology, Amino Acid
EDAT- 2014/05/07 06:00
MHDA- 2014/10/28 06:00
CRDT- 2014/05/07 06:00
PHST- 2014/05/07 06:00 [entrez]
PHST- 2014/05/07 06:00 [pubmed]
PHST- 2014/10/28 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2014 May;30(5):489-92.