PMID- 24796746
OWN - NLM
STAT- MEDLINE
DCOM- 20141027
LR  - 20161125
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 30
IP  - 5
DP  - 2014 May
TI  - [Mechanism of cardiac function changes in rat models of Sjogren's syndrome based 
      on Keap1-Nrf2/ARE signaling pathways].
PG  - 497-501
AB  - OBJECTIVE: To investigate the mechanism underlying the changes in the cardiac 
      function in rat models of Sjogren's syndrome (SS) based on Kelch-like 
      ECH-associated protein 1 (Keap1)-Nrf2/ARE signaling pathways. METHODS: Thirty 
      male Wistar rats were randomly divided into two groups, normal control (NC) group 
      and SS model group, with 15 in each. The rats in the model group were injected 
      with the complete Freund's adjuvant plus homologous antigen of submandibular 
      gland (0.2 mL mixture) into bilateral posterior paw metatarsus. The body mass, 
      water intake, submandibular gland index, spleen index, and histological changes 
      of the glands were observed 30 days after inflammation was induced. Cardiac 
      function was assessed using invasive hemodynamic monitoring. Meanwhile, reactive 
      oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD) and total 
      antioxidant capacity (TAC), IL-18, IL-35 were detected using ELISA; ROS and 
      reactive nitrogen species (RNS), glutathione (GSH), thioredoxin (TrX) protein 
      were determined using immunohistochemical staining. The expressions of Keap 1, 
      nuclear factor erythroid 2-related factor (Nrf2), macrophage activating factor 
      (Maf) antioxidant responsive element (ARE) mRNAs were detected using real-time 
      fluorescent quantitative PCR (qRT-PCR); the levels of heme oxygenase-1 (HO-1), 
      gamma-glutamic acid and a half long glycine synthetase (gamma-GCS) proteins in cardiac 
      tissue were examined using Western blotting. RESULTS: Compared with the NC group, 
      the model group presented reduced body mass, submandibular gland index and spleen 
      index (P<0.05), increased water intake (P<0.01), heart rate (HR), heart index 
      (HI), left ventricular systolic pressure (LVSP) and left ventricular diastolic 
      pressure (LVEDP) (P<0.05), and decreased left ventricular +/-dp/dtmax (P<0.05). 
      Compared with the NC group, the model group had increased IL-18, MDA, RNS, TAC, 
      ROS levels, and Keap1, Maf, Nfr2 mRNAs, HO-1, gamma-GCS protein expressions in the 
      heart tissue, while TrX, GSH, IL-5 and SOD levels decreased significantly 
      (P<0.01). CONCLUSION: The immune imbalance in SS rats may be related with the 
      up-regulated levels of Keap1, Maf, Nfr2 mRNAs, HO-1, gamma-GCS.
FAU - Wang, Fang
AU  - Wang F
AD  - Anhui University of Traditional Chinese Medicine, Hefei 230031, China.
FAU - Liu, Jian
AU  - Liu J
AD  - First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, 
      Hefei 230031, China.
FAU - Ye, Yingfa
AU  - Ye Y
AD  - Anhui University of Traditional Chinese Medicine, Hefei 230031, China.
FAU - Zhang, Xiaojun
AU  - Zhang X
AD  - Anhui University of Traditional Chinese Medicine, Hefei 230031; Hubei University 
      of Chinese Medicine, Wuhan 430065, China.
FAU - Wan, Lei
AU  - Wan L
AD  - First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, 
      Hefei 230031, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Antioxidants)
RN  - 0 (Interleukin-18)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (KEAP1 protein, rat)
RN  - 0 (Kelch-Like ECH-Associated Protein 1)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Nfe2l2 protein, rat)
RN  - 0 (Reactive Oxygen Species)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 52500-60-4 (Thioredoxins)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 6.3.2.2 (Glutamate-Cysteine Ligase)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Animals
MH  - Antioxidants/metabolism/pharmacology
MH  - Blotting, Western
MH  - Disease Models, Animal
MH  - Gene Expression
MH  - Glutamate-Cysteine Ligase/metabolism
MH  - Glutathione/metabolism
MH  - Heart/*physiopathology
MH  - Heme Oxygenase-1/metabolism
MH  - Immunohistochemistry
MH  - Interleukin-18/metabolism
MH  - Intracellular Signaling Peptides and Proteins/*genetics
MH  - Kelch-Like ECH-Associated Protein 1
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - NF-E2-Related Factor 2/*genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Reactive Oxygen Species/metabolism
MH  - Response Elements/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction/*genetics
MH  - Sjogren's Syndrome/*genetics/metabolism/physiopathology
MH  - Superoxide Dismutase/metabolism
MH  - Thioredoxins/metabolism
EDAT- 2014/05/07 06:00
MHDA- 2014/10/28 06:00
CRDT- 2014/05/07 06:00
PHST- 2014/05/07 06:00 [entrez]
PHST- 2014/05/07 06:00 [pubmed]
PHST- 2014/10/28 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2014 May;30(5):497-501.