PMID- 24797612
OWN - NLM
STAT- MEDLINE
DCOM- 20140827
LR  - 20161125
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 545
IP  - 1
DP  - 2014 Jul 15
TI  - Creatine prevents the imbalance of redox homeostasis caused by homocysteine in 
      skeletal muscle of rats.
PG  - 72-9
LID - S0378-1119(14)00537-X [pii]
LID - 10.1016/j.gene.2014.05.005 [doi]
AB  - Homocystinuria is a neurometabolic disease caused by severe deficiency of 
      cystathionine beta-synthase activity, resulting in severe hyperhomocysteinemia. 
      Affected patients present several symptoms including a variable degree of motor 
      dysfunction, being that the pathomechanism is not fully understood. In the 
      present study we investigated the effect of chronic hyperhomocysteinemia on some 
      parameters of oxidative stress, namely 2'7'dichlorofluorescein (DCFH) oxidation, 
      levels of thiobarbituric acid-reactive substances (TBARS), antioxidant enzyme 
      activities (SOD, CAT and GPx), reduced glutathione (GSH), total sulfhydryl and 
      carbonyl content, as well as nitrite levels in soleus skeletal muscle of young 
      rats subjected to model of severe hyperhomocysteinemia. We also evaluated the 
      effect of creatine on biochemical alterations elicited by hyperhomocysteinemia. 
      Wistar rats received daily subcutaneous injection of homocysteine (0.3-0.6 mumol/g 
      body weight), and/or creatine (50mg/kg body weight) from their 6th to the 28th 
      days age. Controls and treated rats were decapitated at 12h after the last 
      injection. Chronic homocysteine administration increased 2'7'dichlorofluorescein 
      (DCFH) oxidation, an index of production of reactive species and TBARS levels, an 
      index of lipoperoxidation. Antioxidant enzyme activities, such as SOD and CAT 
      were also increased, but GPx activity was not altered. The content of GSH, 
      sulfhydril and carbonyl were decreased, as well as levels of nitrite. Creatine 
      concurrent administration prevented some homocysteine effects probably by its 
      antioxidant properties. Our data suggest that the oxidative insult elicited by 
      chronic hyperhomocystenemia may provide insights into the mechanisms by which 
      homocysteine exerts its effects on skeletal muscle function. Creatine prevents 
      some alterations caused by homocysteine.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Kolling, Janaina
AU  - Kolling J
AD  - Laboratorio de Neuroprotecao e Doencas Neurometabolicas, Departamento de 
      Bioquimica, ICBS, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 
      2600-Anexo, CEP 90035-003, Porto Alegre, RS, Brazil; Laboratorio de Erros Inatos 
      do Metabolismo, Departamento de Bioquimica, ICBS, Universidade Federal do Rio 
      Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003, Porto Alegre, RS, 
      Brazil.
FAU - Scherer, Emilene B S
AU  - Scherer EB
AD  - Laboratorio de Neuroprotecao e Doencas Neurometabolicas, Departamento de 
      Bioquimica, ICBS, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 
      2600-Anexo, CEP 90035-003, Porto Alegre, RS, Brazil; Laboratorio de Erros Inatos 
      do Metabolismo, Departamento de Bioquimica, ICBS, Universidade Federal do Rio 
      Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003, Porto Alegre, RS, 
      Brazil.
FAU - Siebert, Cassiana
AU  - Siebert C
AD  - Laboratorio de Neuroprotecao e Doencas Neurometabolicas, Departamento de 
      Bioquimica, ICBS, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 
      2600-Anexo, CEP 90035-003, Porto Alegre, RS, Brazil; Laboratorio de Erros Inatos 
      do Metabolismo, Departamento de Bioquimica, ICBS, Universidade Federal do Rio 
      Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003, Porto Alegre, RS, 
      Brazil.
FAU - Marques, Eduardo Peil
AU  - Marques EP
AD  - Laboratorio de Neuroprotecao e Doencas Neurometabolicas, Departamento de 
      Bioquimica, ICBS, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 
      2600-Anexo, CEP 90035-003, Porto Alegre, RS, Brazil; Laboratorio de Erros Inatos 
      do Metabolismo, Departamento de Bioquimica, ICBS, Universidade Federal do Rio 
      Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003, Porto Alegre, RS, 
      Brazil.
FAU - Dos Santos, Tiago Marcom
AU  - Dos Santos TM
AD  - Laboratorio de Neuroprotecao e Doencas Neurometabolicas, Departamento de 
      Bioquimica, ICBS, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 
      2600-Anexo, CEP 90035-003, Porto Alegre, RS, Brazil; Laboratorio de Erros Inatos 
      do Metabolismo, Departamento de Bioquimica, ICBS, Universidade Federal do Rio 
      Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003, Porto Alegre, RS, 
      Brazil.
FAU - Wyse, Angela T S
AU  - Wyse AT
AD  - Laboratorio de Neuroprotecao e Doencas Neurometabolicas, Departamento de 
      Bioquimica, ICBS, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 
      2600-Anexo, CEP 90035-003, Porto Alegre, RS, Brazil; Laboratorio de Erros Inatos 
      do Metabolismo, Departamento de Bioquimica, ICBS, Universidade Federal do Rio 
      Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003, Porto Alegre, RS, 
      Brazil. Electronic address: wyse@ufrgs.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140502
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Fluoresceins)
RN  - 0 (Nitrites)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 56NQM5UZT1 (2',7'-dichlorofluorescein)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - GAN16C9B8O (Glutathione)
RN  - MU72812GK0 (Creatine)
SB  - IM
MH  - Animals
MH  - Catalase/metabolism
MH  - Creatine/metabolism/*pharmacology
MH  - Female
MH  - Fluoresceins/metabolism
MH  - Glutathione/metabolism
MH  - Glutathione Peroxidase/metabolism
MH  - Homeostasis/drug effects
MH  - Homocysteine/*pharmacology
MH  - Hyperhomocysteinemia/drug therapy/*metabolism
MH  - Male
MH  - Muscle, Skeletal/drug effects/*metabolism
MH  - Nitrites/metabolism
MH  - Oxidation-Reduction
MH  - Rats
MH  - Rats, Wistar
MH  - Superoxide Dismutase/metabolism
MH  - Thiobarbituric Acid Reactive Substances/metabolism
OTO - NOTNLM
OT  - Creatine
OT  - Oxidative stress
OT  - Severe hyperhomocysteinemia
OT  - Soleus skeletal muscle
EDAT- 2014/05/07 06:00
MHDA- 2014/08/29 06:00
CRDT- 2014/05/07 06:00
PHST- 2013/10/07 00:00 [received]
PHST- 2014/03/14 00:00 [revised]
PHST- 2014/05/01 00:00 [accepted]
PHST- 2014/05/07 06:00 [entrez]
PHST- 2014/05/07 06:00 [pubmed]
PHST- 2014/08/29 06:00 [medline]
AID - S0378-1119(14)00537-X [pii]
AID - 10.1016/j.gene.2014.05.005 [doi]
PST - ppublish
SO  - Gene. 2014 Jul 15;545(1):72-9. doi: 10.1016/j.gene.2014.05.005. Epub 2014 May 2.